{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T07:47:11Z","timestamp":1777016831178,"version":"3.51.4"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Cardiac troponin T (cTnT) is key in diagnosing myocardial infarction (MI) but is also elevated in end-stage renal disease (ESRD) patients. Specific larger cTnT proteoforms were identified for the acute phase of MI, while in serum of ESRD patients solely small cTnT fragments were found. However, others allocated this to a pre-analytic effect due to abundant thrombin generation in serum. Therefore, we investigated the effect of various anticoagulation methods on cTnT composition and concentration and compared the cTnT composition of MI and ESRD patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The agreement of cTnT concentrations between simultaneously collected serum, lithium-heparin (LH) plasma, and ethylenediaminetetraacetic acid (EDTA) plasma was studied using the high-sensitivity (hs-)cTnT immunoassay. cTnT proteoform composition was investigated in a standardized time-dependent manner through spike experiments and in simultaneously collected blood matrixes of MI and ESRD patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Excellent hs-cTnT concentration agreements were observed across all blood matrixes (slopes &amp;gt; 0.98; 95% CI, 0.96\u20131.04). Time-dependent degradation (40\u2005kDa intact:29\u2005kDa fragment:15 to 18\u2005kDa fragments) was found in LH plasma and EDTA plasma, and serum in ratios (%) of 90:10:0, 0:5:95, and 0:0:100, respectively (48\u2005h after blood collection). Moreover, gel filtration chromatography (GFC) profiles illustrated mainly larger cTnT proteoforms in MI patients, while in ESRD patients mainly 15 to 18\u2005kDa fragments were found for all matrices.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The extent of cTnT degradation in vitro is dependent on the (anti)coagulation method, without impacting hs-cTnT concentrations. Furthermore, mainly larger cTnT proteoforms were present in MI patients, while in ESRD patients mainly small 15 to 18\u2005kDa cTnT fragments were found. These insights are essential when developing a novel hs-cTnT assay targeting larger cTnT proteoforms.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfae052","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T04:36:09Z","timestamp":1717130169000},"page":"989-1000","source":"Crossref","is-referenced-by-count":4,"title":["Differences in Cardiac Troponin T Composition in Myocardial Infarction and End-Stage Renal Disease Patients: A Blood Tube Effect?"],"prefix":"10.1093","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3668-5083","authenticated-orcid":false,"given":"Wim H M","family":"Vroemen","sequence":"first","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen J S","family":"Denessen","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"},{"name":"CARIM School for Cardiovascular Diseases, Maastricht University , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William P T M","family":"van Doorn","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly E J M","family":"Pelzer","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tilman M","family":"Hackeng","sequence":"additional","affiliation":[{"name":"CARIM School for Cardiovascular Diseases, Maastricht University , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth J R","family":"Litjens","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne M C","family":"Henskens","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"},{"name":"CARIM School for Cardiovascular Diseases, Maastricht University , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank M","family":"van der Sande","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Will K W H","family":"Wodzig","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen P","family":"Kooman","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Nephrology, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto","family":"Bekers","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"},{"name":"CARIM School for Cardiovascular Diseases, Maastricht University , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2008-3328","authenticated-orcid":false,"given":"Douwe","family":"de Boer","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alma M A","family":"Mingels","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory, Maastricht University Medical Center , Maastricht , the Netherlands"},{"name":"CARIM School for Cardiovascular Diseases, Maastricht University , Maastricht , the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"2024090311494890800_jfae052-B1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/eurheartj\/ehy462","article-title":"Fourth universal definition of myocardial infarction (2018)","volume":"40","author":"Thygesen","year":"2019","journal-title":"Eur Heart J"},{"key":"2024090311494890800_jfae052-B2","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1373\/clinchem.2009.132654","article-title":"Analytical validation of a high-sensitivity cardiac troponin T 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