{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:48:06Z","timestamp":1741153686361,"version":"3.38.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T00:00:00Z","timestamp":1735603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Exploring polymorphisms in vitamin D-related genes (VDR) within the Brazilian population provides a valuable model to contribute to the influence of the host genetic variants on chronic viral hepatitis B (CHB).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>126 CHB patients were enrolled in the current study and clinical, laboratory, and 25-hydroxyvitamin D [25(OD)D] level data were obtained. Four VDR (rs7975232, rs1544410, rs10735810, rs731236) and 2 vitamin D-binding protein\/carrier globulin (GC) polymorphisms (rs4588 and rs7041) were determined using TaqMan assays and nucleotide sequencing. Association studies were conducted among viral infection parameters and the patient's genetic variants.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Most patients were male (52.38%) with a mean age of 44.28 (\u00b114.24) years, self-identified as White (32.54%), and exhibited vitamin D insufficiency status (42.06%). The hepatitis B virus (HBV) genotype A was predominant (50%) and 62.7% of the patients had detectable HBV DNA levels \u2264log10 3\u2005IU\/mL. A significant association was observed between HBV genotype A with ApaI and FokI single nucleotide polymorphisms. However, no statistical association between VDR polymorphisms and viral load, viral polymerase mutations, or vitamin D status was found. Vitamin D concentration did not correlate to HBV viral load.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Most HBV-infected individuals presented vitamin D insufficiency, and VDR polymorphism was not associated with virological characteristics except with HBV genotype A, demonstrating that some human genetic signatures are related to HBV genotype distribution.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfae151","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T10:16:02Z","timestamp":1735640162000},"page":"380-391","source":"Crossref","is-referenced-by-count":0,"title":["Lack of Association between Vitamin D Genetic Polymorphism and Virological Characteristics of Hepatitis B Infection"],"prefix":"10.1093","volume":"10","author":[{"given":"J\u00e9ssica C","family":"da Silva","sequence":"first","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda R","family":"Caetano","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana C da F","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia de P","family":"Scalioni","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moyra M","family":"Portilho","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristianne S","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]},{"name":"Federal Institute of Education, Science and Technology , Fortaleza ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa A","family":"Marques","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana C","family":"Miguel","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karis M P","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Federal University of Rio de Janeiro , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia A P","family":"Ivantes","sequence":"additional","affiliation":[{"name":"Curitiba Municipal Health Institution, Center of Guidance and Advisor (COA) , Curitiba ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lia L","family":"Lewis-Ximenez","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-8969","authenticated-orcid":false,"given":"Livia M","family":"Villar","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ , Rio de Janeiro ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,12,31]]},"reference":[{"key":"2025030423061395900_jfae151-B1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3390\/pathogens8020063","article-title":"Hepatitis B virus molecular epidemiology, host-virus interaction, coinfection, and laboratory diagnosis in the MENA region: an update","volume":"8","author":"Al-Sadeq","year":"2019","journal-title":"Pathogens"},{"key":"2025030423061395900_jfae151-B2","doi-asserted-by":"crossref","first-page":"696","DOI":"10.3389\/fgene.2019.00696","article-title":"Host genetic determinants of hepatitis B virus infection","volume":"10","author":"Zhang","year":"2019","journal-title":"Front Genet"},{"key":"2025030423061395900_jfae151-B3","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.3390\/nu12092879","article-title":"Immune modulatory effects of vitamin D on viral infections","volume":"12","author":"Siddiqui","year":"2020","journal-title":"Nutrients"},{"key":"2025030423061395900_jfae151-B4","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1016\/j.biopha.2018.10.140","article-title":"Vitamin D and liver fibrosis: molecular mechanisms and clinical studies","volume":"109","author":"Udomsinprasert","year":"2019","journal-title":"Biomed Pharmacother"},{"key":"2025030423061395900_jfae151-B5","first-page":"6","article-title":"Vitamin D receptor (VDR) gene polymorphism: implications on non-bone diseases","volume":"8","author":"Sakharkar","year":"2017","journal-title":"J Basic Clin Pharm"},{"key":"2025030423061395900_jfae151-B6","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.biopha.2018.10.102","article-title":"Role of vitamin D and vitamin D receptor (VDR) in oral cancer","volume":"109","author":"Fathi","year":"2019","journal-title":"Biomed Pharmacother"},{"key":"2025030423061395900_jfae151-B7","doi-asserted-by":"crossref","first-page":"910","DOI":"10.3389\/fendo.2019.00910","article-title":"Vitamin D binding protein: a historic overview","volume":"10","author":"Bouillon","year":"2020","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2025030423061395900_jfae151-B8","doi-asserted-by":"crossref","first-page":"651","DOI":"10.2217\/pgs-2016-0041","article-title":"Association of vitamin D pathway SNPs and clinical response to interferon in a cohort of HBeAg-negative patients","volume":"18","author":"Cusato","year":"2017","journal-title":"Pharmacogenomics"},{"key":"2025030423061395900_jfae151-B9","doi-asserted-by":"crossref","first-page":"e116026","DOI":"10.1371\/journal.pone.0116026","article-title":"Association of single nucleotide polymorphisms in VDR and DBP genes with HBV-related hepatocellular carcinoma risk in a Chinese population","volume":"9","author":"Peng","year":"2014","journal-title":"PLoS One"},{"key":"2025030423061395900_jfae151-B10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.gene.2017.12.027","article-title":"Association between vitamin D receptor polymorphisms and hepatitis B virus infection susceptibility: a meta-analysis study","volume":"645","author":"He","year":"2018","journal-title":"Gene"},{"key":"2025030423061395900_jfae151-B11","doi-asserted-by":"crossref","first-page":"e0277907","DOI":"10.1371\/journal.pone.0277907","article-title":"The association between vitamin D receptor polymorphism and phases of chronic hepatitis B infection in HBV carriers in Thailand","volume":"17","author":"Ananchuensook","year":"2022","journal-title":"PLoS One"},{"key":"2025030423061395900_jfae151-B12","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3748\/wjg.v27.i3.255","article-title":"Serum vitamin D and vitamin-D-binding protein levels in children with chronic hepatitis B","volume":"27","author":"Huang","year":"2021","journal-title":"World J Gastroenterol"},{"key":"2025030423061395900_jfae151-B13","doi-asserted-by":"crossref","first-page":"E213","DOI":"10.1111\/hepr.12972","article-title":"Association of baseline vitamin D level with genetic determinants and virologic response in patients with chronic hepatitis B","volume":"48","author":"Yu","year":"2018","journal-title":"Hepatol Res"},{"key":"2025030423061395900_jfae151-B14","doi-asserted-by":"crossref","first-page":"300060520910906","DOI":"10.1177\/0300060520910906","article-title":"The association between vitamin D-related gene polymorphisms and hepatitis B virus-related liver cirrhosis","volume":"48","author":"Wu","year":"2020","journal-title":"J Int Med Res"},{"key":"2025030423061395900_jfae151-B15","doi-asserted-by":"crossref","first-page":"e9881","DOI":"10.1097\/MD.0000000000009881","article-title":"Impact of vitamin D receptor and binding protein gene polymorphisms in clinical and laboratory data of HCV patients: cross sectional study","volume":"97","author":"Scalioni","year":"2018","journal-title":"Medicine (Baltimore)"},{"key":"2025030423061395900_jfae151-B16","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1111\/odi.12692","article-title":"Comparison of oral fluid collection methods for the molecular detection of hepatitis B virus","volume":"23","author":"Portilho","year":"2017","journal-title":"Oral Dis"},{"key":"2025030423061395900_jfae151-B17","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol Biol Evol"},{"key":"2025030423061395900_jfae151-B18","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1590\/0004-2730000003388","article-title":"Recomenda\u00e7\u00f5es Da Sociedade Brasileira de Endocrinologia e Metabologia (SBEM) Para o Diagn\u00f3stico e Tratamento Da Hipovitaminose D","volume":"58","author":"Maeda","year":"2014","journal-title":"Arq Bras Endocrinol Metabol"},{"key":"2025030423061395900_jfae151-B19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s12881-019-0903-y","article-title":"Vitamin D receptor ApaI polymorphism associated with progression of liver disease in Vietnamese patients chronically infected with hepatitis B virus","volume":"20","author":"Hoan","year":"2019","journal-title":"BMC Med Genet"},{"key":"2025030423061395900_jfae151-B20","doi-asserted-by":"crossref","first-page":"112447","DOI":"10.1016\/j.nut.2024.112447","article-title":"Potential role of vitamin D in immune response in patients with viral hepatitis","volume":"124","author":"Gerhards","year":"2024","journal-title":"Nutrition"},{"key":"2025030423061395900_jfae151-B21","doi-asserted-by":"crossref","first-page":"15441","DOI":"10.1038\/srep15441","article-title":"Sustained suppression of viral replication in improving vitamin D serum concentrations in patients with chronic hepatitis B","volume":"5","author":"Chen","year":"2015","journal-title":"Sci Rep"},{"key":"2025030423061395900_jfae151-B22","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1002\/hep.26488","article-title":"Low vitamin D serum concentration is associated with high levels of hepatitis B virus replication in chronically infected patients","volume":"58","author":"Farnik","year":"2013","journal-title":"Hepatology"},{"key":"2025030423061395900_jfae151-B23","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1186\/s12879-016-1836-0","article-title":"Association of vitamin D deficiency with hepatitis B virus\u2014related liver diseases","volume":"16","author":"Hoan","year":"2016","journal-title":"BMC Infect Dis"},{"key":"2025030423061395900_jfae151-B24","doi-asserted-by":"crossref","first-page":"135","DOI":"10.3390\/biomedicines11010135","article-title":"Chronic hepatitis B and related liver diseases are associated with reduced 25-hydroxy-vitamin D levels: a systematic review and meta-analysis","volume":"11","author":"Banerjee","year":"2023","journal-title":"Biomedicines"},{"key":"2025030423061395900_jfae151-B25","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1016\/j.jfma.2019.12.004","article-title":"The association of vitamin D with hepatitis B virus replication: bystander rather than offender","volume":"119","author":"Wang","year":"2020","journal-title":"J Formos Med Assoc"},{"key":"2025030423061395900_jfae151-B26","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s10020-018-0055-0","article-title":"Hepatitis B virus downregulates vitamin D receptor levels in hepatoma cell lines, thereby preventing vitamin D-dependent inhibition of viral transcription and production","volume":"24","author":"Gotlieb","year":"2018","journal-title":"Mol Med"},{"key":"2025030423061395900_jfae151-B27","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1007\/s10238-022-00919-9","article-title":"Hepatic vitamin D receptor expression is negatively associated with liver inflammation and fibrosis in patients with chronic HBV infection","volume":"23","author":"Yang","year":"2023","journal-title":"Clin Exp Med"},{"key":"2025030423061395900_jfae151-B28","doi-asserted-by":"crossref","first-page":"e3926","DOI":"10.1097\/MD.0000000000003926","article-title":"Vitamin D serum level is associated with child-pugh score and metabolic enzyme imbalances, but not viral load in chronic hepatitis B patients","volume":"95","author":"Zhao","year":"2016","journal-title":"Medicine (Baltimore)"},{"key":"2025030423061395900_jfae151-B29","doi-asserted-by":"crossref","first-page":"306","DOI":"10.14218\/JCTH.2016.00037","article-title":"Comparison of vitamin D levels in naive, treated, and inactive carriers with chronic hepatitis B virus","volume":"4","author":"Sali","year":"2016","journal-title":"J Clin Transl Hepatol"},{"key":"2025030423061395900_jfae151-B30","doi-asserted-by":"crossref","first-page":"bvac171","DOI":"10.1210\/jendso\/bvac171","article-title":"Epidemiology of vitamin D (EpiVida)\u2014a study of vitamin D status among healthy adults in Brazil","volume":"7","author":"Borba","year":"2023","journal-title":"J Endocr Soc"},{"key":"2025030423061395900_jfae151-B31","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1002\/hep.26962","article-title":"Vitamin D levels in patients with chronic hepatitis B","volume":"60","author":"Efe","year":"2014","journal-title":"Hepatology"},{"key":"2025030423061395900_jfae151-B32","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.3855\/jidc.7459","article-title":"Relationships between low serum vitamin D levels and HBV \u201ca\u201d determinant mutations in chronic hepatitis B patients","volume":"10","author":"Zhu","year":"2016","journal-title":"J Infect Dev Ctries"},{"key":"2025030423061395900_jfae151-B33","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1111\/jgh.12783","article-title":"Association between vitamin D level and viral load or fibrosis stage in chronic hepatitis B patients from Southern China","volume":"30","author":"Yu","year":"2015","journal-title":"J Gastroenterol Hepatol"},{"key":"2025030423061395900_jfae151-B34","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1099\/jgv.0.000789","article-title":"Nationwide overview of the distribution of hepatitis B virus genotypes in Brazil: a 1000-sample multicentre study","volume":"98","author":"Lampe","year":"2017","journal-title":"J Gen Virol"},{"issue":"Suppl 1","key":"2025030423061395900_jfae151-B35","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1016\/j.jhep.2016.01.027","article-title":"Overview of hepatitis B viral replication and genetic variability","volume":"64","author":"Tong","year":"2016","journal-title":"J Hepatol"},{"key":"2025030423061395900_jfae151-B36","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.jhep.2006.01.028","article-title":"Association between vitamin D receptor, CCR5, TNF-\u03b1 and TNF-\u03b2 gene polymorphisms and HBV infection and severity of liver disease","volume":"44","author":"Suneetha","year":"2006","journal-title":"J Hepatol"},{"key":"2025030423061395900_jfae151-B37","doi-asserted-by":"crossref","first-page":"101233","DOI":"10.1016\/j.jbc.2021.101233","article-title":"Vitamin D signaling inhibits HBV activity by directly targeting the HBV core promoter","volume":"297","author":"Ahluwalia","year":"2021","journal-title":"J Biol Chem"}],"container-title":["The Journal of Applied Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jalm\/article-pdf\/10\/2\/380\/61298268\/jfae151.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jalm\/article-pdf\/10\/2\/380\/61298268\/jfae151.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T23:06:25Z","timestamp":1741129585000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jalm\/article\/10\/2\/380\/7935067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,31]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,12,31]]},"published-print":{"date-parts":[[2025,3,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jalm\/jfae151","relation":{},"ISSN":["2475-7241"],"issn-type":[{"type":"electronic","value":"2475-7241"}],"subject":[],"published-other":{"date-parts":[[2025,3]]},"published":{"date-parts":[[2024,12,31]]}}}