{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:48:24Z","timestamp":1767383304665,"version":"3.40.4"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>High-throughput assays are required for novel biomarkers to have clinical potential. Secretoneurin (SN) is a candidate biomarker, and the performance of a new high-throughput SN assay is not known.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We measured SN concentrations with a prototype chemiluminescent immunoassay (CLIA) in 299 patients hospitalized with acute dyspnea. We compared the results with a CE-marked SN enzyme linked immunosorbent assay (ELISA). We adjudicated the cause of dyspnea as heart failure (HF) or non-HF, and we obtained information on all-cause mortality during follow-up.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>SN concentrations measured with CLIA and ELISA were closely correlated: rho = 0.81, P &amp;lt; 0.001. SN CLIA concentrations were higher in HF patients (n = 129) compared to patients with non-HF-related dyspnea (n = 170): median 51 (quartile 1\u20133 40\u201369) vs 41 (32\u201354) pmol\/L, P &amp;lt; 0.001. The area under the curve (AUC) of SN CLIA to diagnose HF was 0.64 (95% CI, 0.58\u20130.71) and the AUC of N-terminal pro-B-type natriuretic peptide (NT-proBNP) was 0.85 (0.81\u20130.89). During median 818 days follow-up, 110 patients died (37%). There was a nonlinear association between SN CLIA concentrations and mortality with optimal cutpoint 53\u2005pmol\/L. SN CLIA concentrations &amp;gt;53\u2005pmol\/L were associated with mortality after adjusting for clinical variables and NT-proBNP and cardiac troponin T concentrations: hazard ratio 1.7 (95% CI, 1.1\u20132.7), AUC 0.67 (0.61\u20130.74). We found similar results for SN ELISA for diagnosis and prognosis with AUC 0.63 (0.57\u20130.70) for the prediction of mortality.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The high-throughput SN CLIA correlates with the SN ELISA and provides independent prognostic information over established biomarkers in patients with acute dyspnea.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfaf011","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T01:25:41Z","timestamp":1741656341000},"page":"691-703","source":"Crossref","is-referenced-by-count":1,"title":["Secretoneurin Concentrations Measured with a High-Throughput Assay and Clinical Outcomes in Patients Hospitalized with Acute Dyspnea: Data from the Akershus Cardiac Examination 2 Study"],"prefix":"10.1093","volume":"10","author":[{"given":"Helge","family":"R\u00f8sj\u00f8","sequence":"first","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilde","family":"Rugolo","sequence":"additional","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]},{"name":"Institute for Experimental Medical Research, Oslo University Hospital , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica","family":"Gj\u00f8rven","sequence":"additional","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]},{"name":"Department of Cardiology, Division of Medicine, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne L","family":"Faaren","sequence":"additional","affiliation":[{"name":"CardiNor AS , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Frantzen","sequence":"additional","affiliation":[{"name":"CardiNor AS , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geir","family":"Christensen","sequence":"additional","affiliation":[{"name":"Institute for Experimental Medical Research, Oslo University Hospital , Oslo ,","place":["Norway"]},{"name":"Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne Didrik","family":"H\u00f8iseth","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Division of Medicine, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anett H","family":"Ottesen","sequence":"additional","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Bhatnagar","sequence":"additional","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]},{"name":"Department of Cardiology, Division of Medicine, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus N","family":"Lyngbakken","sequence":"additional","affiliation":[{"name":"Akershus Clinical Research Center (ACR), Division of Research and Innovation, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]},{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]},{"name":"Department of Cardiology, Division of Medicine, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torbj\u00f8rn","family":"Omland","sequence":"additional","affiliation":[{"name":"K. G. Jebsen Center for Cardiac Biomarkers, Institute of Clinical Medicine, University of Oslo , Oslo ,","place":["Norway"]},{"name":"Department of Cardiology, Division of Medicine, Akershus University Hospital , L\u00f8renskog ,","place":["Norway"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"2025050206370665800_jfaf011-B1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1161\/CIRCULATIONAHA.106.683110","article-title":"Benchmarks for the assessment of novel cardiovascular biomarkers","volume":"115","author":"Morrow","year":"2007","journal-title":"Circulation"},{"key":"2025050206370665800_jfaf011-B2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jacc.2014.10.065","article-title":"Secretoneurin is a novel prognostic cardiovascular biomarker associated with cardiomyocyte calcium handling","volume":"65","author":"Ottesen","year":"2015","journal-title":"J Am Coll Cardiol"},{"key":"2025050206370665800_jfaf011-B3","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.clinbiochem.2019.06.008","article-title":"Circulating secretoneurin concentrations in patients with moderate to severe aortic stenosis","volume":"71","author":"Brynildsen","year":"2019","journal-title":"Clin Biochem"},{"key":"2025050206370665800_jfaf011-B4","doi-asserted-by":"crossref","first-page":"e412","DOI":"10.1097\/CCM.0000000000003670","article-title":"Circulating secretoneurin concentrations after cardiac surgery: data from the FINNish acute kidney injury heart study","volume":"47","author":"Brynildsen","year":"2019","journal-title":"Crit Care Med"},{"key":"2025050206370665800_jfaf011-B5","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1159\/000517444","article-title":"Performance of a novel research-use-only secretoneurin ELISA in patients with suspected acute coronary syndrome: comparison with an established secretoneurin radioimmunoassay","volume":"146","author":"Myhre","year":"2021","journal-title":"Cardiol"},{"key":"2025050206370665800_jfaf011-B6","doi-asserted-by":"crossref","first-page":"110595","DOI":"10.1016\/j.clinbiochem.2023.110595","article-title":"Prognostic value of secretoneurin in chronic heart failure. 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