{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:14:50Z","timestamp":1792012490453,"version":"4.4.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005633","name":"Finnish Foundation for Cardiovascular Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005633","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinical Research Fund"},{"DOI":"10.13039\/501100022793","name":"Turku University Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022793","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022340","name":"Finnish Society of Clinical Chemistry","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022340","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Varsinais-Suomi Regional Fund of the Finnish Cultural Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cardiac troponins (cTn) are used as diagnostic biomarkers of acute myocardial infarction (MI), but elevated levels of cTn can also be observed in other conditions. We report here the design and analytical evaluation of an immunoassay that targets intact and mildly fragmented forms of cardiac troponin T (referred to as long cTnT), which has been shown to better differentiate between MI and end-stage renal disease than the current Roche Elecsys\u00ae high sensitivity cTnT assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The long cTnT assay was evaluated for analytical characteristics. Serum and heparin plasma sample matrices were compared and the analyte stability was studied by storing endogenous long cTnT from samples of ST-segment elevation MI patients in heparin plasma or buffer at different temperatures and subjecting samples to freeze\u2013thaw cycles. The correlation of long cTnT levels and time after MI symptom onset was also studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The long cTnT assay achieved a limit of detection of 10.8\u2005ng\/L and a lower limit of quantitation (10% CV) of 30.8\u2005ng\/L. The response was linear from 5 to 5000\u2005ng\/L. Serum produced significantly lower results than heparin plasma. Endogenous long cTnT tolerated freeze\u2013thaw cycles, but stability was compromised when stored at higher temperatures. The fraction of circulating long cTnT was highest during early hours of MI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The long cTnT assay presented good analytical performance. Our results support using heparin plasma as the sample material and avoiding prolonged sample storing at room temperatures. Long cTnT fraction decreases in time after the onset of type 1 MI.<\/jats:p>\n                    <jats:p>ClinicalTrials.gov registration: NCT04465591<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jalm\/jfaf143","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T11:50:31Z","timestamp":1756900231000},"page":"36-47","source":"Crossref","is-referenced-by-count":1,"title":["Design and Analytical Evaluation of an Immunoassay for Long Forms of Cardiac Troponin T"],"prefix":"10.1093","volume":"11","author":[{"given":"Rami","family":"Aalto","sequence":"first","affiliation":[{"name":"Biotechnology Unit, Department of Life Technologies, University of Turku , Turku ,","place":["Finland"]},{"name":"Clinical Chemistry, Tyks Laboratories, Turku University Hospital , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akseli","family":"Lahtinen","sequence":"additional","affiliation":[{"name":"Biotechnology Unit, Department of Life Technologies, University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K E Juhani","family":"Airaksinen","sequence":"additional","affiliation":[{"name":"Heart Center, Turku University Hospital and University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuija","family":"Vasankari","sequence":"additional","affiliation":[{"name":"Heart Center, Turku University Hospital and University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tapio","family":"Hellman","sequence":"additional","affiliation":[{"name":"Kidney Center, Turku University Hospital and University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Koskim\u00e4ki","sequence":"additional","affiliation":[{"name":"Biotechnology Unit, Department of Life Technologies, University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7886-3477","authenticated-orcid":false,"given":"Saara","family":"Wittfooth","sequence":"additional","affiliation":[{"name":"Biotechnology Unit, Department of Life Technologies, University of Turku , Turku ,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,9,30]]},"reference":[{"key":"2026010511270533000_jfaf143-B1","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1373\/clinchem.2009.130799","article-title":"Increases of cardiac troponin in conditions other than acute coronary syndrome and heart failure","volume":"55","author":"Kelley","year":"2009","journal-title":"Clin Chem"},{"key":"2026010511270533000_jfaf143-B2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ijcard.2019.08.019","article-title":"Cardiac troponin elevations in marathon runners. 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The MaraCat Study","volume":"295","author":"Paana","year":"2019","journal-title":"Int J Cardiol"},{"key":"2026010511270533000_jfaf143-B3","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1093\/clinchem\/hvae075","article-title":"Highly sensitive immunoassay for long forms of cardiac troponin T using upconversion luminescence","volume":"70","author":"Salonen","year":"2024","journal-title":"Clin Chem"},{"key":"2026010511270533000_jfaf143-B4","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1093\/clinchem\/hvae182","article-title":"Design and analytical evaluation of novel cardiac troponin assays targeting multiple forms of the cardiac troponin I\u2013cardiac troponin T\u2013troponin C complex and fragmentation forms","volume":"71","author":"Li","year":"2024","journal-title":"Clin Chem"},{"key":"2026010511270533000_jfaf143-B5","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1373\/clinchem.2018.301127","article-title":"Full-size and partially truncated cardiac 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