{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:21:01Z","timestamp":1788877261696,"version":"build-2803163510"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T00:00:00Z","timestamp":1775433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vitamin D holds important roles in human physiology, and deficiency of this vitamin is of worldwide clinical significance. Serum levels of 25-hydroxyvitamin D (25-OHD) are currently the most appropriate indicator of vitamin D status in the majority of individuals, and thus its preanalytical stability and accurate measurement in clinical laboratories are of significant interest.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed previously published data from the Vitamin D Standardization Programme commutability study and 2 further datasets: one retrospective observational study and one prospective experiment exploring additional aspects of 25-OHD stability. We analyzed each dataset through the use of robust Bayesian multilevel linear models using the {brms} package in the R statistical computing environment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our analyses provided evidence that 25-OHD was stable when shipped at ambient temperature, as the average difference between shipping at frozen vs ambient temperature on total 25-OHD measurements across multiple methods, laboratories, and samples [with assigned values ranging from 12.1 to 41.4\u2005ng\/mL (30.2\u2013103.6\u2005nmol\/L)] was \u22120.3\u2005ng\/mL [\u22120.8\u2005nmol\/L; 95% credible interval (CI) \u22123.5\u20131.7\u2005nmol\/L]. Our analyses of orthogonal datasets corroborated these findings, with the estimated difference of storage for 7 days at ambient vs frozen temperature prior to shipping being 0.4\u2005ng\/mL (0.9\u2005nmol\/L; 95% CI \u22120.1\u20131.9\u2005nmol\/L).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The data and analyses we present here provide substantial evidence to support 25-OHD stability when shipped at ambient temperature and thus support the continuation of this practice as part of the Vitamin D External Quality Assurance Scheme (DEQAS) quality program.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jalm\/jfag034","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T11:45:51Z","timestamp":1775130351000},"page":"786-797","source":"Crossref","is-referenced-by-count":1,"title":["Samples for the Determination of Total 25-Hydroxyvitamin D Levels Were Stable at Ambient Temperature"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2457-1467","authenticated-orcid":false,"given":"Edmund H","family":"Wilkes","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, North West London Pathology, Imperial College Healthcare NHS Trust, Charing Cross Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, North West London Pathology, Imperial College Healthcare NHS Trust, Charing Cross Hospital , London ,","place":["United Kingdom"]},{"name":"DEQAS (Vitamin D External Quality Assessment Scheme), Imperial College Healthcare NHS Trust, Charing Cross Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma L","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, North West London Pathology, Imperial College Healthcare NHS Trust, Charing Cross Hospital , London ,","place":["United Kingdom"]},{"name":"DEQAS (Vitamin D External Quality Assessment Scheme), Imperial College Healthcare NHS Trust, Charing Cross Hospital , London ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,6]]},"reference":[{"key":"2026070109530555000_jfag034-B1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.3390\/nu17020277","article-title":"Vitamin D: evidence-based health benefits and recommendations for population guidelines","volume":"17","author":"Grant","year":"2025","journal-title":"Nutrients"},{"key":"2026070109530555000_jfag034-B2","doi-asserted-by":"crossref","first-page":"783","DOI":"10.3390\/nu17050783","article-title":"Vitamin D supplementation: practical 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