{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T15:28:46Z","timestamp":1787153326212,"version":"build-2736575974"},"reference-count":20,"publisher":"Oxford University Press (OUP)","funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["NU50CK000423"],"award-info":[{"award-number":["NU50CK000423"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["U60OE000103"],"award-info":[{"award-number":["U60OE000103"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["CK14-140105PPHF18"],"award-info":[{"award-number":["CK14-140105PPHF18"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>To reduce the spread of carbapenem-resistant gram-negative bacteria, the Centers for Disease Control recommends rectal and perirectal swab surveillance using the Cepheid Xpert\u00ae Carba-R Assay. An expressed limitation of the assay is lack of validation data for patients &amp;lt;21 years. To address this limitation, an in-house validation was performed using pediatric stool specimens from patients &amp;lt;21 years.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pediatric validation of the Cepheid Copan Dual Swab Collection Device and Xpert\u00ae Carba-R Assay was performed using pooled stool specimens from 17 patients ages &amp;lt;1 to 21 years, spiked with Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, Enterobacter cloacae, and Pseudomonas aeruginosa harboring \u03b2-lactamase mechanisms (Klebsiella pneumoniae carbapenemase, New Delhi metallo-\u03b2-lactamase, oxacillinase-48-like carbapenemase, Verona integron-encoded metallo-\u03b2-lactamase, and imipenemase). Validation was supported by molecular and culture analysis of 747 pediatric clinical specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Pediatric stool-spiked rectal swabs produced Cepheid Carba-R results with 100% accuracy (SD &amp;lt; 1; CV &amp;lt; 4%) and excellent precision (SD &amp;lt; 1, CV &amp;lt; 4%). Clinical carbapenemase surveillance of &amp;gt;24 000 rectal specimens screened by the Carba-R assay (2018\u20132024) showed lower carbapenemase-detected percent positivity in patients ages 0 to 20 years (3.0%\u20134.2%) compared with adults ages 21 to &amp;gt;65 (7.8%\u201312.7%) (\u03c72, P &amp;lt; 0.00001). No significant age-group differences were observed in the ability to isolate detected \u03b2-lactamase mechanisms from patient specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The Cepheid Copan Dual Swab Collection Device with the Xpert\u00ae Carba-R Assay is a valid method for rapid (&amp;lt;1\u2005h) screening of carbapenemase-producing organisms (\u03b2-lactamase mechanisms: Klebsiella pneumoniae carbapenemase, New Delhi metallo-\u03b2-lactamase, oxacillinase-48-like carbapenemase, Verona integron-encoded metallo-\u03b2-lactamase, and imipenemase) in pediatric patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jalm\/jfag127","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:40:29Z","timestamp":1784893229000},"source":"Crossref","is-referenced-by-count":0,"title":["Validation of Rectal Swab Specimens for Sensitive and Specific Carbapenemase Gene Detection in Pediatric Patients"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3902-2234","authenticated-orcid":false,"given":"Majie C","family":"Foster","sequence":"first","affiliation":[{"name":"New York State Department of Health, Wadsworth Center , Albany, NY ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailee","family":"Cummings","sequence":"additional","affiliation":[{"name":"New York State Department of Health, Wadsworth Center , Albany, NY ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon L","family":"Morris","sequence":"additional","affiliation":[{"name":"New York State Department of Health, Wadsworth Center , Albany, NY ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J","family":"Nazarian","sequence":"additional","affiliation":[{"name":"New York State Department of Health, Wadsworth Center , Albany, NY ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberlee A","family":"Musser","sequence":"additional","affiliation":[{"name":"New York State Department of Health, Wadsworth Center , Albany, NY ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026081911131656800_jfag127-B1","author":"Centers for Disease Control and Prevention"},{"key":"2026081911131656800_jfag127-B2","author":"Centers for Disease Control and Prevention"},{"key":"2026081911131656800_jfag127-B3","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1080\/21505594.2016.1255381","article-title":"Screening for carbapenem-resistant Enterobacteriaceae: who, when, and 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