{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T19:08:03Z","timestamp":1648926483916},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:00:00Z","timestamp":1579910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Carbamazepine is an effective drug for treating seizures and trigeminal neuralgia. Therapeutic drug monitoring of free carbamazepine in serum can be useful in situations that drug--protein binding is altered to guide regimen adjustment and to aid in the diagnosis of clinical toxicity.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Separation of the nonprotein bound carbamazepine was achieved via ultrafiltration through a molecular weight cut-off filter. A method for free carbamazepine measurement was developed on the automated cobas chemistry analyzers (Roche Diagnostics) by modifying the Carbamazepine Gen 4 assay (Roche Diagnostics). Assay performance characteristics were established including precision, accuracy, reportable range, analytical specificity, and stability.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The intra- and inter-assay imprecision was 0%-1.4% and 2.4%-5.1%, respectively. The lower limit of quantitation was 0.3\u2009\u00b5g\/mL, and the assay was linear up to 10.0\u2009\u00b5g\/mL. A spike recovery study, using reference standard material, showed recovery was 93.5%--101.3% across the analytical measurement range. Method comparison with a reference laboratory method demonstrated equivalent performance with a slope of 1.01, intercept of 0.09, and correlation coefficient of 0.9948.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This assay provides a simple and accurate method for monitoring free carbamazepine with a fast turnaround time.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jalm\/jfz003","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T13:16:48Z","timestamp":1579958208000},"page":"357-362","source":"Crossref","is-referenced-by-count":1,"title":["Development and Validation of a Free Carbamazepine Assay on an Automated Chemistry Analyzer"],"prefix":"10.1093","volume":"5","author":[{"given":"Xiaochun","family":"Zhang","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Boyert","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Mitro","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Tang","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Bowers","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam J","family":"McShane","sequence":"first","affiliation":[{"name":"Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,1,25]]},"reference":[{"key":"2020030410500589800_jfz003-B1","doi-asserted-by":"crossref","first-page":"276.","DOI":"10.3389\/fphar.2016.00276","article-title":"Current status of the new antiepileptic drugs in chronic pain","volume":"7","author":"Sidhu","year":"2016","journal-title":"Front Pharmacol"},{"key":"2020030410500589800_jfz003-B2","first-page":"CD001911","article-title":"Carbamazepine versus phenytoin monotherapy for epilepsy: an individual participant data review","volume":"2","author":"Nevitt","year":"2017","journal-title":"Cochrane Database Syst Rev"},{"key":"2020030410500589800_jfz003-B3","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1111\/j.1528-1167.2008.01561.x","article-title":"Antiepileptic drugs\u2013best practice guidelines for therapeutic drug monitoring: a position paper by the subcommission on therapeutic drug monitoring, ILAE commission on therapeutic strategies","volume":"49","author":"Patsalos","year":"2008","journal-title":"Epilepsia"},{"key":"2020030410500589800_jfz003-B4","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1093\/clinchem\/44.5.1085","article-title":"Standards of laboratory practice: antiepileptic drug monitoring. National academy of clinical biochemistry","volume":"44","author":"Warner","year":"1998","journal-title":"Clin Chem"},{"key":"2020030410500589800_jfz003-B5","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.4155\/bio.15.99","article-title":"A LC-MS\/MS method for therapeutic drug monitoring of carbamazepine, lamotrigine and valproic acid in DBS","volume":"7","author":"Linder","year":"2015","journal-title":"Bioanalysis"},{"key":"2020030410500589800_jfz003-B6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1358\/mf.2001.23.9.662144","article-title":"Determination of carbamazepine and its metabolite carbamazepine-10, 11-epoxide in serum with gas-chromatography mass spectrometry","volume":"23","author":"Minkova","year":"2001","journal-title":"Methods Find Exp Clin Pharmacol"},{"key":"2020030410500589800_jfz003-B7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0920-1211(02)00182-1","article-title":"Overtreatment in epilepsy: adverse consequences and mechanisms","volume":"52","author":"Perucca","year":"2002","journal-title":"Epilepsy Res"}],"container-title":["The Journal of Applied Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/5\/2\/357\/32784517\/jfz003.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jalm\/article-pdf\/5\/2\/357\/32784517\/jfz003.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T15:50:19Z","timestamp":1583337019000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jalm\/article\/5\/2\/357\/5716021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,25]]},"references-count":7,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,1,25]]},"published-print":{"date-parts":[[2020,3,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jalm\/jfz003","relation":{},"ISSN":["2576-9456","2475-7241"],"issn-type":[{"value":"2576-9456","type":"print"},{"value":"2475-7241","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,3]]},"published":{"date-parts":[[2020,1,25]]}}}