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Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) system, as an emerging IVD method, plays a crucial role in the field of infectious disease detection due to its superior sensitivity and specificity. Recently, an increasing number of scientists have been devoted to improving the performance of CRISPR-based detection and on-site point-of-care testing (POCT) from extraction-free detection, amplification-free, modified Cas\/crRNA complexes, quantitative assays, one-pot detection, and multiplexed platform. In this review, we describe the potential roles of these novel approaches and platforms in one-pot methods, quantitative molecular diagnostics as well as multiplexed detection. This review will not only help guide the full use of the CRISPR-Cas tools for quantification, multiplexed detection, POCT and as next-generation diagnostic biosensing platforms but also inspire new ideas, technological advances, and engineering strategies to address real-world challenges like the ongoing COVID-19 pandemic.<\/jats:p>","DOI":"10.1093\/jambio\/lxad035","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T19:23:51Z","timestamp":1677093831000},"source":"Crossref","is-referenced-by-count":23,"title":["Progress and bioapplication of CRISPR-based one-step, quantitative and multiplexed infectious disease diagnostics"],"prefix":"10.1093","volume":"134","author":[{"given":"Yue","family":"Shen","sequence":"first","affiliation":[{"name":"College of Public Health, Zhengzhou University , Zhengzhou 450000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Hu","sequence":"additional","affiliation":[{"name":"Laboratory Biosafety Technology Center, Henan Academy of Medical Sciences , Zhengzhou 450046 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Public Health, Zhengzhou University , Zhengzhou 450000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangcai","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Public Health, Zhengzhou University , Zhengzhou 450000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjun","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory Biosafety Technology Center, Henan Academy of Medical Sciences , Zhengzhou 450046 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6129-0310","authenticated-orcid":false,"given":"Shuaiyin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Public Health, Zhengzhou University , Zhengzhou 450000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,22]]},"reference":[{"key":"2023031616462186700_","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1089\/crispr.2019.0011","article-title":"Nucleic acid detection of plant genes using CRISPR-Cas13","volume":"2","author":"Abudayyeh","year":"2019","journal-title":"The CRISPR Journal"},{"key":"2023031616462186700_","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/s41586-020-2279-8","article-title":"Massively multiplexed nucleic acid detection with 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