{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:06Z","timestamp":1787009046758,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":18,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003524","name":"Ministry of Business, Innovation and Employment","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003524","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Strategic Science Investment Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The aim of this study was to investigate dual far-UVC (Ultraviolet-C) (222\u00a0nm) and blue LED (Light Emitting Diode) (405\u00a0nm) light on the inactivation of extended spectrum \u03b2-lactamase-producing Escherichia coli (ESBL-Ec) and to determine if repetitive exposure to long pulses of light resulted in changes to light tolerance, and antibiotic susceptibility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Antimicrobial efficiency of dual and individual light wavelengths and development of light tolerance in E. coli was evaluated through a spread plate method after exposure to light at 25\u00a0cm. Dual light exposure for 30\u00a0min resulted in a 5\u20136 log10 CFU mL\u22121 reduction in two ESBL-Ec and two antibiotic-sensitive control E. coli strains. The overall inhibition achieved by dual light treatment was always greater than the combined reductions (log10 CFU) observed from exposure to individual light wavelengths (combined 222\u2013405\u00a0nm), indicating a synergistic relationship between blue LED and far-UVC light when used together. Repetitive long pulses of dual and individual far-UVC light exposure resulted in light tolerance in two ESBL-Ec strains but not the antibiotic-sensitive E. coli strains. Subsequent passages of repetitive light-treated ESBL-Ec strains continued to exhibit light tolerance. Antibiotic susceptibility was determined through a standard disk diffusion method. No changes were observed in the antibiotic susceptibility profiles for any of the four strains after exposure to either dual or individual wavelengths.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Dual light exposure was effective in the disinfection of ESBL-Ec in solution; however, antibiotic-resistant E. coli were able to develop light tolerance after repetitive exposure to light.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxad124","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T15:03:28Z","timestamp":1686668608000},"source":"Crossref","is-referenced-by-count":6,"title":["Light tolerance of extended spectrum \u03b2-lactamase producing\n                    <i>Escherichia coli<\/i>\n                    strains after repetitive exposure to far-UVC and blue LED light"],"prefix":"10.1093","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0546-7748","authenticated-orcid":false,"given":"Amanda","family":"Gardner","sequence":"first","affiliation":[{"name":"Food Systems Integrity Team, AgResearch Ltd., Hopkirk Research Institute, Cnr University Ave and Library Road, Massey University , Palmerston North 4442 , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aswathi","family":"Soni","sequence":"additional","affiliation":[{"name":"Food Systems Integrity Team, AgResearch Ltd., Hopkirk Research Institute, Cnr University Ave and Library Road, Massey University , Palmerston North 4442 , New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Cookson","sequence":"additional","affiliation":[{"name":"Food Systems Integrity Team, AgResearch Ltd., Hopkirk Research Institute, Cnr University Ave and Library Road, Massey University , Palmerston North 4442 , New 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