{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:16:26Z","timestamp":1787170586343,"version":"build-2736575974"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42150410383"],"award-info":[{"award-number":["42150410383"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Li Ka Shing Foundation Cross-Disciplinary Research","award":["2020LKSFG03E"],"award-info":[{"award-number":["2020LKSFG03E"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study aimed to investigate the high-resolution phenotypic and genotypic characterization of extended-spectrum \u03b2-lactamase (ESBL)-producing Escherichia coli strains isolated from hospitalized patients to explore the resistance genes and mobile genetic elements (MGEs) involved in horizontal dissemination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Between May and September 2021, a total of 216 ESBL-producing E. coli isolates were recovered from multiple departments. The identification of strains was performed using MALDI-TOF mass spectrometry and PCR, while antibiotic susceptibility testing was carried out using the Vitek 2 COMPACT system to determine resistance patterns, while PCR was used to detect different resistance genes and MGEs. In addition, a conjugation assay was performed to investigate the horizontal gene transfer of resistance genes. Selected isolates underwent whole-genome sequencing (WGS) using the Illumina MiSeq platform.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 216 out of 409 E. coli isolates recovered from a tertiary hospital were observed to be ESBL-producing, giving a carriage rate of 52.8%, as determined by phenotypic screening. The most frequent sources of ESBL-producing E. coli isolates were urine (129\/216, 59.72%) and blood (50\/216, 23.14%). The most prevalent ESBL genes identified were blaCTX-M (60.18%), blaTEM (40.27%), and blaSHV (18.05%). Three E. coli isolates were found to carry the genes blaNDM, mcr-1, and fosA3 genes. The most prevalent MGEs were IS26 (95.37%), Int (87.03%), and IncFIB (76.85%). WGS analysis of eight MDR E. coli strains revealed that these isolates belonged to eight different sequence types (STs) and serotypes and were found to harbor multiple plasmid replicons and virulence factors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study highlights a high incidence of antibiotic resistance genes and MGEs associated with the dissemination of ESBLs and other resistance genes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxad125","type":"journal-article","created":{"date-parts":[[2023,6,17]],"date-time":"2023-06-17T06:54:15Z","timestamp":1686984855000},"source":"Crossref","is-referenced-by-count":6,"title":["Characterization of antibiotic resistance genes and mobile elements in extended-spectrum \u03b2-lactamase-producing\n                    <i>Escherichia coli<\/i>\n                    strains isolated from hospitalized patients in Guangdong, China"],"prefix":"10.1093","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4346-5903","authenticated-orcid":false,"given":"Muhammad","family":"Shafiq","sequence":"first","affiliation":[{"name":"Research Institute of Clinical Pharmacy, Shantou University Medical College , Shantou, Guangdong 515041 , China"},{"name":"Department of Cell Biology and Genetics, Shantou University Medical College , Shantou 515041 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazrat","family":"Bilal","sequence":"additional","affiliation":[{"name":"Department of Dermatology, The Second Affiliated Hospital of Shantou University Medical College , Shantou 515041 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Budi","family":"Permana","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, University of Queensland , Brisbane City QLD 4072 , 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