{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:15:08Z","timestamp":1793193308207,"version":"4.5.0"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"SA Water Corporation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>To demonstrate the capability of wastewater-based surveillance (WBS) as a tool for detecting potential cases of Japanese Encephalitis Virus (JEV) infection in the community.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>In this study, we explore the potential of WBS to detect cases of JEV infection by leveraging from an established SARS-CoV-2 wastewater surveillance program. We describe the use of two reverse transcriptase quantitative polymerase chain reaction (RTqPCR) assays targeting JEV to screen archived samples from two wastewater treatment plants (WWTPs). JEV was detected in wastewater samples collected during a timeframe coinciding with a cluster of acute human encephalitis cases, alongside concurrent evidence of JEV detection in mosquito surveillance and the sentinel chicken programs within South Australia's Riverland and Murraylands regions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Current surveillance measures for JEV encounter multiple constraints, which may miss the early stages of JEV circulation or fail to capture the full extent of transmission. The detection of JEV in wastewater during a disease outbreak highlights the potential WBS has as a complementary layer to existing monitoring efforts forming part of the One Health approach required for optimal disease response and control.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxad275","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T05:44:09Z","timestamp":1700113449000},"source":"Crossref","is-referenced-by-count":17,"title":["The detection of Japanese encephalitis virus in municipal wastewater during an acute disease outbreak"],"prefix":"10.1093","volume":"134","author":[{"given":"Stella","family":"Fanok","sequence":"first","affiliation":[{"name":"South Australian Water Corporation , Adelaide 5001, SA , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T","family":"Monis","sequence":"additional","affiliation":[{"name":"South Australian Water Corporation , Adelaide 5001, SA , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra R","family":"Keegan","sequence":"additional","affiliation":[{"name":"South Australian Water Corporation , Adelaide 5001, SA , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendon J","family":"King","sequence":"additional","affiliation":[{"name":"South Australian Water Corporation , Adelaide 5001, SA , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,17]]},"reference":[{"key":"2023120619201222100_bib1","doi-asserted-by":"crossref","first-page":"S294","DOI":"10.1093\/infdis\/jiu384","article-title":"Environmental surveillance for polioviruses in the Global Polio Eradication Initiative","volume":"30","author":"Asghar","year":"2014","journal-title":"J Infect Dis"},{"key":"2023120619201222100_bib2","doi-asserted-by":"crossref","first-page":"8018","DOI":"10.1038\/s41598-018-26333-4","article-title":"Detection of Japanese Encephalitis virus RNA in human throat samples in Laos\u2013a pilot study","volume":"8","author":"Bharucha","year":"2018","journal-title":"Sci Rep"},{"key":"2023120619201222100_bib3","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1021\/acs.estlett.1c00517","article-title":"Persistence of dengue (Serotypes 2 and 3), Zika, yellow fever, and murine hepatitis virus RNA in untreated wastewater","volume":"8","author":"Chandra","year":"2021","journal-title":"Environ Sci Technol Lett"},{"key":"2023120619201222100_bib4","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.tig.2011.04.003","article-title":"Strategies for viral RNA stability: live long and prosper","volume":"27","author":"Dickson","year":"2011","journal-title":"Trends Genet"},{"key":"2023120619201222100_bib5","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-59403-8_2","article-title":"Japanese Encephalitis Virus: ecology and Epidemiology","volume-title":"Japanese Encephalitis and West Nile Viruses. 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