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By optimizing the culture medium and fermentation time, 72\u00a0h of fermentation in chemically defined medium (CDM) medium was determined as the optimal fermentation conditions. Based on two promoters commonly used in Streptococcus (ldhp and xylS1p), eight promoter refactoring strains were constructed, nevertheless all showed impaired antioxidant production. In-frame deletion and complementation experiments demonstrated the positive regulatory role of mao1 and mao2, on mao. Afterward, the mao1 and mao2, overexpression strain S. mutans UA140\/pDL278:: mao1mao2, were constructed, in which the production of mutantioxidin was improved significantly.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In this study, through a combination of varied strategies such as optimization of fermentation conditions and overexpression of regulatory genes, production of mutantioxidin was increased by 10.5 times ultimately.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxae017","type":"journal-article","created":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T08:14:48Z","timestamp":1706170488000},"source":"Crossref","is-referenced-by-count":1,"title":["Production improvement of an antioxidant in cariogenic <i>Streptococcus mutans<\/i> 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