{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:04:39Z","timestamp":1753877079633,"version":"3.41.2"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ016231"],"award-info":[{"award-number":["PJ016231"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>This study aimed to develop a sensitive and specific recombinant antigen protein indirect enzyme-linked immunosorbent assay (ELISA) kit to detect the Shiga toxin (Stx)-producing Escherichia coli (STEC) antibodies against porcine edema disease (ED).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>The recombinant antigen was co-expressed with the STEC-derived Stx2e A2-fragment and Stx2e B protein in E. coli BL21(DE3) pLysS cells and purified using maltose-binding protein open columns. We used a Shiga-like toxin 2 antibody to test the specificity of the recombinant antigen in an indirect ELISA, which was detected in antigen-coated wells but not in uncoated wells. We tested the indirect ELISA system using samples from the STEC-immunized pig group, the commercial swine farm group, and healthy aborted fetal pleural effusion group; five and twenty samples, respectively, were positive for STEC in the former, whereas all three samples were negative for STEC in the latter.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This newly developed indirect ELISA may be a specific method for diagnosing STEC infections in pigs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxae021","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T17:35:38Z","timestamp":1706549738000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of an indirect ELISA system for diagnosis of porcine edema disease using recombinant modified Stx2e antigen"],"prefix":"10.1093","volume":"135","author":[{"given":"Jin","family":"Hur","sequence":"first","affiliation":[{"name":"Department of Veterinary Public Health, College of Veterinary Medicine, Jeonbuk National University , Iksan-si, Jeollabuk-do 54596 , Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho-Kyoung","family":"Jung","sequence":"additional","affiliation":[{"name":"CTCVAC Inc. , 106, Saengmyeonggwahakgwan-gil, Hongcheon-eup, Hongcheon-gun, Gangwon-do 25142 , Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung-Won","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Daegu Catholic University , Gyeongsan-si, Gyeongsangbuk-do 38430 , Republic of 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