{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T20:05:27Z","timestamp":1789243527240,"version":"build-2803163510"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,4,27]],"date-time":"2024-04-27T00:00:00Z","timestamp":1714176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1C1C1014695"],"award-info":[{"award-number":["NRF-2020R1C1C1014695"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1A2C3005357"],"award-info":[{"award-number":["NRF-2020R1A2C3005357"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>This study aimed to develop an editable structural scaffold for improving drug development, including pharmacokinetics and pharmacodynamics of antibiotics by using synthetic compounds derived from a (hetero)aryl-quinoline hybrid scaffold.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>In this study, 18 CF3-substituted (hetero)aryl-quinoline hybrid molecules were examined for their potential antibacterial activity against Staphylococcus aureus by determining minimal inhibitory concentrations. These 18 synthetic compounds represent modifications to key regions of the quinoline N-oxide scaffold, enabling us to conduct a structure-activity relationship analysis for antibacterial potency. Among the compounds, 3\u00a0m exhibited potency against with both methicillin resistant S. aureus strains, as well as other Gram-positive bacteria, including Enterococcus faecalis and Bacillus subtilis. We demonstrated that 3\u00a0m disrupted the bacterial proton motive force (PMF) through monitoring the PMF and conducting the molecular dynamics simulations. Furthermore, we show that this mechanism of action, disrupting PMF, is challenging for S. aureus to overcome. We also validated this PMF inhibition mechanism of 3\u00a0m in an Acinetobacter baumannii strain with weaken lipopolysaccharides. Additionally, in Gram-negative bacteria, we demonstrated that 3\u00a0m exhibited a synergistic effect with colistin that disrupts the outer membrane of Gram-negative bacteria.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our approach to developing editable synthetic novel antibacterials underscores the utility of CF3-substituted (hetero)aryl-quinoline scaffold for designing compounds targeting the bacterial proton motive force, and for further drug development, including pharmacokinetics and pharmacodynamics.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxae104","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T11:48:19Z","timestamp":1714045699000},"source":"Crossref","is-referenced-by-count":4,"title":["Development of novel indole\u2013quinoline hybrid molecules targeting bacterial proton motive force"],"prefix":"10.1093","volume":"135","author":[{"given":"Jinbeom","family":"Seo","sequence":"first","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University , Suwon 16419 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hoon","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University , Suwon 16419 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayoung","family":"Ko","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University , Suwon 16419 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Sungkyunkwan University , Suwon 16419 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeongwon","family":"Moon","sequence":"additional","affiliation":[{"name":"School of 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