{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T15:11:27Z","timestamp":1787325087350,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T00:00:00Z","timestamp":1717804800000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203531"],"award-info":[{"award-number":["82203531"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2023J01625"],"award-info":[{"award-number":["2023J01625"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013064","name":"Key Research and Development Program of Jiangxi Province","doi-asserted-by":"publisher","award":["20202BBGL73023"],"award-info":[{"award-number":["20202BBGL73023"]}],"id":[{"id":"10.13039\/501100013064","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Irritable bowel syndrome (IBS) is a prevalent gastrointestinal disorder, encompassing diarrhea-predominant irritable bowel syndrome (IBS-D). Here, we utilized 16S rDNA gene sequencing to identify potential microbial drivers of IBS-D.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>A total of 30 healthy relatives and 27 patients with IBS-D were recruited. Clinical data and fecal samples were collected from patients and controls. 16S rDNA gene sequencing was performed to obtain fecal bacterial data. Differences in community composition were evaluated utilizing analysis of similarity (ANOSIM) using Bray\u2013Curtis dissimilarity. The Wilcoxon rank sum test was used to compare differences in taxa and functional pathways. Finally, the key gut microbiota was identified using the random forest algorithm. Gut microbiota diversity, estimated through the Observe, Chao1, and abundance-based coverage estimator (ACE) indices, was significantly lower in the IBS-D patients than in the healthy relatives. ANOSIM analysis further confirmed significant differences in the composition of the gut microbiota between IBS-D patients and healthy relatives, with an R value of 0.106 and a P-value of 0.005. Notably, the IBS-D patients exhibited a significant enrichment of specific bacterial genera, including Fusicatenibacter, Streptococcus, and Klebsiella, which may possess potential pathogenic properties. In particular, the bacterial genus Klebsiella demonstrated a positive correlation with irritable bowel syndrome severity scoring system scores. Conversely, healthy subjects showed enrichment of bacterial genera such as Alistipes, Akkermansia, and Dialister, which may be beneficial bacteria in IBS-D. Utilizing the random forest model, we developed a discriminative model for IBS-D based on differential bacterial genera. This model exhibited impressive performance, with an area under the curve value of 0.90. Additionally, our analysis did not reveal any gender-specific differences in the microbiota community composition among IBS-D patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings offer preliminary insights into the potential relationship between intestinal microbiota and IBS-D. The identification model for IBS-D, grounded in gut microbiota, holds promising prospects for improving early diagnosis of IBS-D.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxae118","type":"journal-article","created":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T00:34:29Z","timestamp":1715474069000},"source":"Crossref","is-referenced-by-count":11,"title":["Gut microbial signatures of patients with diarrhea-predominant irritable bowel syndrome and their healthy relatives"],"prefix":"10.1093","volume":"135","author":[{"given":"Jie","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Northern Jiangsu People\u2019s Hospital , Yangzhou 225001 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Constipation, Chengdu Anorectal Hospital , Chengdu 610000 , 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Traditional Chinese Medicine , Fuzhou 350003 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy, The First Affiliated Hospital of Fujian Medical University , Fuzhou 350005 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuyang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Affiliated Lianyungang Hospital of Xuzhou Medical University\/The First People's Hospital of Lianyungang , Lianyungang 222061 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,7]]},"reference":[{"key":"2024070814454614000_bib1","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.3390\/nu13051506","article-title":"Diarrhea predominant-irritable bowel syndrome (IBS-D): effects of different nutritional patterns on intestinal dysbiosis and 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