{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:19:53Z","timestamp":1770743993589,"version":"3.49.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307895\/2020-3"],"award-info":[{"award-number":["307895\/2020-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["425107\/2018-3"],"award-info":[{"award-number":["425107\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Currently, immunoinformatic approaches have shown promise in rapidly and cost-effectively identifying new antigens from the Leptospira proteome. Chimeric multiepitope proteins offer a strategy with significant potential for implementation in diagnosis and vaccines development.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>In this study, we detail the immunoinformatic analyses and design of a new recombinant chimeric protein constructed with epitopes identified from the sequences of ErpY-like and LemA proteins, previously identified as potential antigens for controlling leptospirosis. We expressed the chimeric protein using Escherichia coli heterologous systems, evaluated its antigenicity using serum from naturally infected patients, and its immunogenicity in mice as an animal model, with Freund as an adjuvant. The resulting recombinant chimeric protein, named rErpY-LemA, was successfully expressed and purified using a prokaryotic system, with an expected mass of 35\u00a0kDa. Serologic assays using serum samples from naturally infected patients demonstrated recognition of the chimera protein by antibodies present in sera. Animals immunized with the chimera exhibited a significant IgG antibody response from the 7th day (P\u00a0&amp;lt;\u00a00.001), persisting until day 49 of experimentation, with a titer of 1:12,800 (P\u00a0&amp;lt;\u00a00.05). Notably, significant production of IgA, IgM, and IgG subclasses was observed in animals immunized with the chimera.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These results highlight the promising role of immunoinformatics in rapidly identifying antigens and the potential of chimeric multiepitope proteins in developing effective strategies for leptospirosis control.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxae179","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T21:23:27Z","timestamp":1721165007000},"source":"Crossref","is-referenced-by-count":4,"title":["Immunoinformatic approaches for ErpY-LemA chimeric protein design for use in leptospirosis control"],"prefix":"10.1093","volume":"135","author":[{"given":"Elsa Giovanna","family":"\u00c1vila-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas , CEP 96010-900, Pelotas, RS , Brazil"},{"name":"Biotechnology Nucleus, Technological Development Center, Federal University of Pelotas , CEP 96010-900, Pelotas, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thayn\u00e1 Laner","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas , CEP 96010-900, Pelotas, RS , Brazil"},{"name":"Biotechnology Nucleus, Technological Development Center, Federal University of Pelotas , CEP 96010-900, Pelotas, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel Ladeira","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, Institute of Biology, Federal University of Pelotas , CEP 96010-900, Pelotas, RS , Brazil"},{"name":"Biotechnology Nucleus, 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