{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:05:52Z","timestamp":1790906752950,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T00:00:00Z","timestamp":1727740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFC1703200"],"award-info":[{"award-number":["2017YFC1703200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005892","name":"China Academy of Chinese Medical Sciences","doi-asserted-by":"publisher","award":["CI2022E001XB"],"award-info":[{"award-number":["CI2022E001XB"]}],"id":[{"id":"10.13039\/501100005892","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81974579"],"award-info":[{"award-number":["81974579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Health Science and Technology Capacity Improvement Project of Jilin Province","award":["2022JC042"],"award-info":[{"award-number":["2022JC042"]}]},{"name":"Jilin Provincial Central Committee Supported by Medical Standardization Project","award":["zybz-2022-001"],"award-info":[{"award-number":["zybz-2022-001"]}]},{"name":"Jilin Provincial Clinical Research Center of Pediatrics of Traditional Chinese Medicine","award":["20200603008SF"],"award-info":[{"award-number":["20200603008SF"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To explore the therapeutic potential of Forsythoside B in treating Streptococcus pneumoniae (S. pneumoniae) infections, focusing on its ability to inhibit pneumolysin activity and protect cells from damage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Hemolysis tests were used to evaluate Forsythoside B\u2019s inhibitory effect on pneumolysin activity, while growth curve analysis assessed its impact on S. pneumoniae growth. Western blotting and oligomerization analysis were conducted to examine its influence on pneumolysin oligomerization. Cytotoxicity assays, including LDH release and live\/dead cell staining, evaluated the protective effects of Forsythoside B against pneumolysin-induced damage in A549 cells. Additionally, a mouse model was employed to test the effects on survival rates, lung bacterial load, and inflammation. The results showed that Forsythoside B significantly inhibited pneumolysin activity, reduced its oligomerization, and protected A549 cells from damage without affecting bacterial growth. In the mouse model, it improved survival rates and reduced lung inflammation, indicating its potential as a therapeutic agent against S. pneumoniae infections.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Forsythoside B shows potential as a therapeutic agent for treating pneumonia, particularly in infections caused by S. pneumoniae.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxae251","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T07:16:58Z","timestamp":1727939818000},"source":"Crossref","is-referenced-by-count":2,"title":["Forsythoside B, the active component of\n                    <i>Frosythiae fructuse<\/i>\n                    water extract, alleviates\n                    <i>Streptococcus pneumoniae<\/i>\n                    virulence by targeting pneumolysin"],"prefix":"10.1093","volume":"135","author":[{"given":"Zhongtian","family":"Wang","sequence":"first","affiliation":[{"name":"College of Traditional Chinese Medicine, Changchun University of Chinese Medicine , No. 1035, Boshuo Road, Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Sun","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Changchun University of Chinese Medicine , No. 1035, Boshuo Road, Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Tong","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhui","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Changchun University of Chinese Medicine , No. 1035, Boshuo Road, Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fushuang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Changchun University of Chinese Medicine , No. 1035, Boshuo Road, Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Changchun University of Chinese Medicine , No. 1035, Boshuo Road, Jingyue National High-Tech Industrial Development Zone, Changchun, Jilin 130117 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Ding","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Sun","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lie","family":"Wang","sequence":"additional","affiliation":[{"name":"Children\u2019s Diagnosis and Treatment Center, The Affiliated Hospital to Changchun University of Chinese Medicine , No. 185, Shenzhen Street, Nanguan District, Changchun, Jilin 130022 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"2024101810024942000_bib1","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1007\/s10517-024-05993-5","article-title":"Recombinant pneumolysin of pneumococci induces TLR4 expression and maturation of dendritic cells in vitro","volume":"176","author":"Akhmatova","year":"2023","journal-title":"Bull Exp Biol Med"},{"key":"2024101810024942000_bib2","doi-asserted-by":"publisher","first-page":"e01710","DOI":"10.1128\/mBio.01710-14","article-title":"Pneumolysin activates macrophage lysosomal membrane permeabilization and executes apoptosis by distinct mechanisms without membrane pore formation","volume":"5","author":"Bewley","year":"2014","journal-title":"mBio"},{"key":"2024101810024942000_bib3","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.neuint.2019.02.008","article-title":"Forsythiaside prevents \u03b2-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-\u03baB pathway","volume":"125","author":"Chen","year":"2019","journal-title":"Neurochem Int"},{"key":"2024101810024942000_bib4","doi-asserted-by":"publisher","first-page":"e0282970","DOI":"10.1371\/journal.pone.0282970","article-title":"Pneumolysin as a target for new therapies against pneumococcal infections: a systematic review","volume":"18","author":"Cima\u00a0Cabal","year":"2023","journal-title":"PLoS One"},{"key":"2024101810024942000_bib5","doi-asserted-by":"publisher","first-page":"38013","DOI":"10.1038\/srep38013","article-title":"Streptococcus pneumoniae disrupts pulmonary immune defence via elastase release following pneumolysin-dependent neutrophil lysis","volume":"6","author":"Domon","year":"2016","journal-title":"Sci Rep"},{"key":"2024101810024942000_bib6","doi-asserted-by":"publisher","first-page":"615959","DOI":"10.3389\/fcimb.2021.615959","article-title":"The role of neutrophils and neutrophil elastase in pneumococcal pneumonia","volume":"11","author":"Domon","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"2024101810024942000_bib7","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1093\/femsre\/fux037","article-title":"A visual review of the human pathogen Streptococcus pneumoniae","volume":"41","author":"Engholm","year":"2017","journal-title":"FEMS Microbiol Rev"},{"key":"2024101810024942000_bib8","doi-asserted-by":"publisher","first-page":"222","DOI":"10.3389\/fcimb.2020.00222","article-title":"A pangenome approach for discerning species-unique gene markers for identifications of Streptococcus pneumoniae and Streptococcus pseudopneumoniae","volume":"10","author":"Gonzales-Siles","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"2024101810024942000_bib9","doi-asserted-by":"publisher","first-page":"114133","DOI":"10.1016\/j.jep.2021.114133","article-title":"The TCM prescription Ma-xing-shi-gan-tang inhibits Streptococcus pneumoniae pathogenesis by targeting pneumolysin","volume":"275","author":"Guo","year":"2021","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib10","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1111\/j.1365-2249.2004.02611.x","article-title":"The role of pneumolysin in pneumococcal pneumonia and meningitis","volume":"138","author":"Hirst","year":"2004","journal-title":"Clin Exp Immunol"},{"key":"2024101810024942000_bib11","doi-asserted-by":"publisher","first-page":"113275","DOI":"10.1016\/j.jep.2020.113275","article-title":"Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4\/MyD88\/NF-\u03baB and TGF-\u03b2\/smads signaling pathways","volume":"262","author":"Hu","year":"2020","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib12","doi-asserted-by":"publisher","first-page":"4754","DOI":"10.1111\/febs.15001","article-title":"Type I IFN expression is stimulated by cytosolic MtDNA released from pneumolysin-damaged mitochondria via the STING signaling pathway in macrophages","volume":"286","author":"Hu","year":"2019","journal-title":"FEBS J"},{"key":"2024101810024942000_bib13","doi-asserted-by":"publisher","first-page":"1831","DOI":"10.3390\/nu11081831","article-title":"Forsythia suspensa protects against bone loss in ovariectomized mice","volume":"11","author":"Hwang","year":"2019","journal-title":"Nutrients"},{"key":"2024101810024942000_bib14","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.jep.2017.05.011","article-title":"Anti-inflammatory effects of Forsythia suspensa in dextran sulfate sodium-induced colitis","volume":"206","author":"Hwang","year":"2017","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib15","doi-asserted-by":"publisher","first-page":"33561","DOI":"10.1073\/pnas.2015368117","article-title":"Macrophage LC3-associated phagocytosis is an immune defense against Streptococcus pneumoniae that diminishes with host aging","volume":"117","author":"Inomata","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024101810024942000_bib16","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1002\/ptr.3668","article-title":"Forsythoside B protects against experimental sepsis by modulating inflammatory factors","volume":"26","author":"Jiang","year":"2012","journal-title":"Phytother Res"},{"key":"2024101810024942000_bib17","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1186\/s12974-020-01967-2","article-title":"Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-\u03baB signaling in Alzheimer\u2019s disease","volume":"17","author":"Kong","year":"2020","journal-title":"J Neuroinflammation"},{"key":"2024101810024942000_bib18","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/j.jep.2017.07.015","article-title":"Antiviral effect of forsythoside a from Forsythia suspensa (Thunb.) Vahl fruit against influenza a virus through reduction of viral M1 protein","volume":"209","author":"Law","year":"2017","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib19","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1002\/emmm.200900025","article-title":"Streptococcus pneumoniae evades human dendritic cell surveillance by pneumolysin expression","volume":"1","author":"Littmann","year":"2009","journal-title":"EMBO Mol Med"},{"key":"2024101810024942000_bib20","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.intimp.2018.11.033","article-title":"Protective effect of forsythoside B against lipopolysaccharide-induced acute lung injury by attenuating the TLR4\/NF-\u03baB pathway","volume":"66","author":"Liu","year":"2019","journal-title":"Int Immunopharmacol"},{"key":"2024101810024942000_bib21","doi-asserted-by":"publisher","first-page":"4217","DOI":"10.1016\/j.psj.2020.05.011","article-title":"Forsythia suspensa extract enhances performance via the improvement of nutrient digestibility, antioxidant status, anti-inflammatory function, and gut morphology in broilers","volume":"99","author":"Long","year":"2020","journal-title":"Poult Sci"},{"key":"2024101810024942000_bib22","doi-asserted-by":"publisher","first-page":"104683","DOI":"10.1016\/j.micpath.2020.104683","article-title":"Dryocrassin ABBA ameliorates Streptococcus pneumoniae-induced infection in vitro through inhibiting Streptococcus pneumoniae growth and neutralizing pneumolysin activity","volume":"150","author":"Lv","year":"2021","journal-title":"Microb Pathog"},{"key":"2024101810024942000_bib23","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1111\/j.1469-0691.2010.03183.x","article-title":"Streptococcus pneumoniae: virulence factors and variation","volume":"16","author":"Mitchell","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"2024101810024942000_bib24","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.2147\/DDDT.S470685","article-title":"Forsythoside B mitigates monocrotaline-induced pulmonary arterial hypertension via blocking the NF-\u03baB signaling pathway to attenuate vascular remodeling [LETTER]","volume":"18","author":"Pasiana","year":"2024","journal-title":"Drug Des Devel Ther"},{"key":"2024101810024942000_bib25","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1111\/jam.14769","article-title":"Betulin attenuates pneumolysin-induced cell injury and DNA damage","volume":"130","author":"Qi","year":"2021","journal-title":"J Appl Microbiol"},{"key":"2024101810024942000_bib26","doi-asserted-by":"publisher","first-page":"22972","DOI":"10.1038\/srep22972","article-title":"Pneumococcal pneumolysin induces DNA damage and cell cycle arrest","volume":"6","author":"Rai","year":"2016","journal-title":"Sci Rep"},{"key":"2024101810024942000_bib27","doi-asserted-by":"publisher","first-page":"105025","DOI":"10.1016\/j.bioorg.2021.105025","article-title":"Neuroprotective and anti-inflammatory phenylethanoidglycosides from the fruits of Forsythia suspensa","volume":"113","author":"Shao","year":"2021","journal-title":"Bioorg Chem"},{"key":"2024101810024942000_bib28","first-page":"9971","article-title":"Deep genome annotation of the opportunistic human pathogen Streptococcus pneumoniae D39","volume":"46","author":"Slager","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"2024101810024942000_bib29","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1007\/s10482-017-0880-0","article-title":"Juglone alleviates pneumolysin-induced human alveolar epithelial cell injury via inhibiting the hemolytic activity of pneumolysin","volume":"110","author":"Song","year":"2017","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2024101810024942000_bib30","doi-asserted-by":"publisher","first-page":"174354","DOI":"10.1016\/j.ejphar.2021.174354","article-title":"Forsythiaside B inhibits myocardial fibrosis via down regulating TGF-\u03b21\/Smad signaling pathway","volume":"908","author":"Sun","year":"2021","journal-title":"Eur J Pharmacol"},{"key":"2024101810024942000_bib31","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.jep.2016.04.015","article-title":"Forsythia suspensa fruit extracts and the constituent matairesinol confer anti-allergic effects in an allergic dermatitis mouse model","volume":"187","author":"Sung","year":"2016","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib32","doi-asserted-by":"publisher","first-page":"112563","DOI":"10.1016\/j.ejmech.2020.112563","article-title":"A review on the structure and pharmacological activity of phenylethanoid glycosides","volume":"209","author":"Tian","year":"2021","journal-title":"Eur J Med Chem"},{"key":"2024101810024942000_bib33","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1016\/S0140-6736(09)61114-4","article-title":"Pathogenesis, treatment, and prevention of pneumococcal pneumonia","volume":"374","author":"van\u00a0der\u00a0Poll","year":"2009","journal-title":"Lancet North Am Ed"},{"key":"2024101810024942000_bib34","doi-asserted-by":"publisher","first-page":"7915","DOI":"10.1021\/acs.nanolett.6b04219","article-title":"Unraveling the pore-forming steps of pneumolysin from Streptococcus pneumoniae","volume":"16","author":"van\u00a0Pee","year":"2016","journal-title":"Nano Lett"},{"key":"2024101810024942000_bib35","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.jep.2017.08.040","article-title":"Phytochemistry, pharmacology, quality control and future research of Forsythia suspensa (Thunb.) Vahl: a review","volume":"210","author":"Wang","year":"2018","journal-title":"J Ethnopharmacol"},{"key":"2024101810024942000_bib36","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1099\/00222615-48-9-863","article-title":"Detection of pneumolysin in sputum","volume":"48","author":"Wheeler","year":"1999","journal-title":"J Med Microbiol"},{"key":"2024101810024942000_bib37","doi-asserted-by":"publisher","first-page":"170074","DOI":"10.1098\/rsob.170074","article-title":"Sequencing of the variable region of rpsB to discriminate between Streptococcus pneumoniae and other streptococcal species","volume":"7","author":"Wyllie","year":"2017","journal-title":"Open Biol"},{"key":"2024101810024942000_bib38","doi-asserted-by":"publisher","first-page":"109120","DOI":"10.1016\/j.intimp.2022.109120","article-title":"Forsythoside B attenuates neuro-inflammation and neuronal apoptosis by inhibition of NF-\u03baB and p38-MAPK signaling pathways through activating Nrf2 post spinal cord injury","volume":"111","author":"Xia","year":"2022","journal-title":"Int Immunopharmacol"},{"key":"2024101810024942000_bib39","doi-asserted-by":"publisher","first-page":"112298","DOI":"10.1016\/j.phytochem.2020.112298","article-title":"Labdane diterpenoids from Forsythia suspensa with anti-inflammatory and anti-viral activities","volume":"173","author":"Xiang","year":"2020","journal-title":"Phytochemistry"},{"key":"2024101810024942000_bib40","doi-asserted-by":"publisher","first-page":"103651","DOI":"10.1016\/j.bioorg.2020.103651","article-title":"Anti-inflammatory and anti-viral labdane diterpenoids from the fruits of Forsythia suspensa","volume":"96","author":"Zhao","year":"2020","journal-title":"Bioorg Chem"},{"key":"2024101810024942000_bib41","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1124\/mol.115.100610","article-title":"Verbascoside alleviates pneumococcal pneumonia by reducing pneumolysin oligomers","volume":"89","author":"Zhao","year":"2016","journal-title":"Mol Pharmacol"},{"key":"2024101810024942000_bib42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.lfs.2017.04.002","article-title":"Shikonin alleviates the biotoxicity produced by pneumococcal pneumolysin","volume":"177","author":"Zhao","year":"2017","journal-title":"Life Sci"},{"key":"2024101810024942000_bib43","doi-asserted-by":"publisher","first-page":"693983","DOI":"10.3389\/fphar.2021.693983","article-title":"Phenylethanoid glycosides from Callicarpa kwangtungensis Chun attenuate TNF-\u03b1-induced cell damage by inhibiting NF-\u03baB pathway and enhancing Nrf2 pathway in A549 cells","volume":"12","author":"Zheng","year":"2021","journal-title":"Front Pharmacol"},{"key":"2024101810024942000_bib44","doi-asserted-by":"publisher","first-page":"704260","DOI":"10.3389\/fphar.2021.704260","article-title":"UPLC\/Q-TOF MS screening and identification of antibacterial compounds in Forsythia suspensa (Thunb.) vahl leaves","volume":"12","author":"Zhou","year":"2021","journal-title":"Front Pharmacol"},{"key":"2024101810024942000_bib45","doi-asserted-by":"publisher","first-page":"170074","DOI":"10.3390\/ijms22083880","article-title":"Tissue-specific accumulation and isomerization of valuable phenylethanoid glycosides from Plantago and Forsythia plants","volume":"22","author":"Z\u00fcrn","year":"2021","journal-title":"Int J Mol Sci"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxae251\/59597752\/lxae251.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/135\/10\/lxae251\/59863848\/lxae251.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/135\/10\/lxae251\/59863848\/lxae251.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T06:03:12Z","timestamp":1729231392000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxae251\/7811328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxae251","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,10]]},"published":{"date-parts":[[2024,10]]},"article-number":"lxae251"}}