{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:39:47Z","timestamp":1772138387818,"version":"3.50.1"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"United States Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["#WIS06036"],"award-info":[{"award-number":["#WIS06036"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aimed to characterize and compare the antimicrobial resistance profiles of clinically relevant bacterial taxa isolated from biting stable flies (Stomoxys spp.) and bovine manure samples collected at a dairy research facility over the course of an entire fly breeding season. The presence of extended-spectrum beta-lactamase and other antimicrobial resistance genes (ARGs) was also examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>A total of 606 fly- and 180 manure-derived strains were tested via disk diffusion for susceptibility to commonly administered antibiotics used in veterinary and human medicine. A small percentage of Enterobacterales exhibited resistance to the tested antimicrobials, including ceftiofur and other beta-lactam antibiotics. Extended spectrum beta-lactamase genes (TEM, CTX, OXA, CMY) were detected by polymerase chain reaction (PCR) amplification in ceftiofur-resistant Escherichia coli, Klebsiella, and Enterobacter spp. isolates. We additionally identified pirlimycin-resistant Staphylococcus and Mammaliicoccus spp. isolates encoding lnuA, a lincosamide resistance gene found primarily on small mobilizable plasmids.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings highlight the significance of stable flies in the carriage of antimicrobial-resistant bacterial strains and plasmid-associated ARGs on dairy farms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf025","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T23:21:04Z","timestamp":1738192864000},"source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of antimicrobial resistance phenotypes and genes in stable fly- and manure-derived bacterial isolates from clinically relevant taxa in dairy settings"],"prefix":"10.1093","volume":"136","author":[{"given":"Andrew 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