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Additionally, the study evaluated whether the inclusion of growth promoters affected the recovery of multi-drug-resistant isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>A total of 144 isolates were obtained from rectal swabs on Simmons citrate agar supplemented with 1% inositol. Of these, 45 non-replicative isolates underwent extensive characterization, including molecular and phenotypic analyses. Sequencing identified that 77% were Klebsiella pneumoniae, 14.5% K. aerogenes, and 8.5% K. variicola. Isolates exhibiting the same polymorphic profiles were detected across different animals and treatments, with and without AGP. Seventy-one percent were multidrug-resistant, as determined by disk diffusion testing. The isolates harbored genes such as mcr-1, blaCTX-M-2, sul2, tetB, qnrS, and dfrA, among others. Additionally, genes encoding siderophores like enterobactin, aerobactin, and yersiniabactin were detected via Polymerase Chain Reaction (PCR). Thirty-nine isolates were strong biofilm producers, 45% moderate, and 16% weak in vitro tests. The predominant genetic profiles included single, double, or triple-locus variants of ST25, ST147, and ST4691. Two novel sequence types were identified: ST7694 (K. pneumoniae) and ST7699 (K. variicola). Survival and persistence analyses in Galleria mellonella showed that these isolates exhibited a virulent phenotype and an enhanced capacity for multiplication in the early hours of infection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Clinically healthy swine act as reservoirs for multidrug-resistant Klebsiella spp. exhibiting significant virulence phenotypes. The identification of novel sequence types contributes to epidemiological surveillance and the One Health framework.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf029","type":"journal-article","created":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T00:57:51Z","timestamp":1738976271000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>Klebsiella<\/i> spp. in healthy pigs: reservoirs of antimicrobial resistance and potential pathogenic threats"],"prefix":"10.1093","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-429X","authenticated-orcid":false,"given":"J\u00e9ssica Nogueira","family":"Rosa","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Universidade Federal de Vi\u00e7osa , 36570-900, Vi\u00e7osa, Minas Gerais ,","place":["Brazil"]},{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular de Bact\u00e9rias, Instituto de Biotecnologia Aplicada \u00e0 Agropecu\u00e1ria\u2014Bioagro, Universidade Federal de 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