{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:17:51Z","timestamp":1778051871978,"version":"3.51.4"},"reference-count":109,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Indonesian Endowment Fund for Education","award":["20141122022186"],"award-info":[{"award-number":["20141122022186"]}]},{"name":"MarieCurie ITN BluePharmTrain"},{"name":"LPDP\/BRIN","award":["B-1738\/II.7.5\/FR\/11\/2022"],"award-info":[{"award-number":["B-1738\/II.7.5\/FR\/11\/2022"]}]},{"name":"LPDP\/BRIN","award":["B-2699\/III.5\/PR.03.08\/11\/2022"],"award-info":[{"award-number":["B-2699\/III.5\/PR.03.08\/11\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>This study aims to assess the antimicrobial activity of sponges from Lembeh Strait, explore their prokaryotic diversity, and investigate correlations between sponge antimicrobial activities and their associated prokaryotic communities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>Secondary metabolites were extracted from sixty two sponge tissues using MeOH: EtOAc as solvent (1:1). Antimicrobial activity of the sponge crude extracts was tested against Escherichia coli, Aeromonas salmonicida, Bacillus subtilis, Staphylococcus simulans, Saprolegnia parasitica, and Candida oleophila. The strongest antimicrobial activity was observed for sponge extracts from Theonella sp. against Sa. parasitica. Microbial community profiling was performed for sponges and environmental samples (seawater and sediment) using 16S rRNA gene amplicon sequencing. Distinct prokaryotic community compositions were observed in sponges compared to their environments, with a strong influence of host-identity. A significant positive correlation between the phylum Actinobacteriota and sponge antimicrobial activity suggests importance of Actinobacteriota for sponge\u2019s bioactive properties. In addition, six amplicon sequence variants (ASVs) belonging to the phyla Actinobacteriota, Chloroflexota, Rhodothermota, and candidate phylum PAUC34f showed significant positive correlations with particular sponge antimicrobial activities, indicating these taxa as interesting targets for future bioprospecting endeavours.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study highlights sponges from the Lembeh Strait, Indonesia, as producers of antimicrobial compounds and the importance of host identity on sponge-prokaryotic community composition. Moreover, poorly studied taxa such as candidate phylum PAUC34f and Chloroflexota members might represent interesting targets for future bioprospecting endeavours.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf129","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T16:56:07Z","timestamp":1748019367000},"source":"Crossref","is-referenced-by-count":4,"title":["Sponges from Lembeh Strait: antimicrobial activity and prokaryotic diversity"],"prefix":"10.1093","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4863-629X","authenticated-orcid":false,"given":"Akhirta","family":"Atikana","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University & Research , Stippeneng 4, 6708 WE, Wageningen ,","place":["The Netherlands"]},{"name":"Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN) , Jln Raya Jakarta-Bogor KM 46, KST Soekarno, Cibinong, 16911, Bogor ,","place":["Indonesia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna","family":"Gutleben","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography , 9500 Gilman Drive, La Jolla, 92093, CA ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"de\u00a0Voogd","sequence":"additional","affiliation":[{"name":"Marine Biodiversity, Naturalis Biodiversity Centre , Darwinweg 2, 2333 CR, Leiden ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tjalling","family":"Tjalsma","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University & Research , Stippeneng 4, 6708 WE, Wageningen ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan Li","family":"Nio","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University & Research , Stippeneng 4, 6708 WE, Wageningen ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Versluis","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Wageningen University & Research , Stippeneng 4, 6708 WE, Wageningen ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anak Agung Gede","family":"Indraningrat","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Health Sciences, Warmadewa University , Jl. 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