{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:04:02Z","timestamp":1791331442920,"version":"4.3.1"},"reference-count":106,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005627","name":"University of Li\u00e8ge","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005627","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis, a fish disease highly aggressive for salmonids and responsible for significant economic losses in aquaculture worldwide. This study aimed to explore genomic and antimicrobial resistance traits of Western European A.\u00a0salmonicida subsp. salmonicida strains and to develop an adapted infection model using larvae of the greater wax moth Galleria mellonella to assess the pathogenic potential of this psychrophilic subspecies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Three A.\u00a0salmonicida subsp. salmonicida strains, isolated from salmonids displaying clinical signs of furunculosis, were tested against a panel of antibiotics and sequenced to characterize their genome. Virulence was evaluated in G. mellonella larvae using bacterial doses ranging from 101 to 106 CFU\/larva. Two isolates exhibited multidrug resistance to antibiotics commonly used against furunculosis. Although closely related to the reference strain A449, genomic analyses revealed multiple plasmids known to encode antibiotic resistance genes. Virulence assays showed that this subspecies was lethal at doses as low as 101 CFU\/larva, and that a fully functional Type III secretion system (T3SS) is not essential for the infection of G.\u00a0mellonella, likely due to the presence of other virulence factors in T3SS-deficient strains.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings enhance the genomic characterization of European A.\u00a0salmonicida subsp. salmonicida and validate the use of G. mellonella larvae as a relevant alternative infection model for studying this psychrophilic subspecies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf193","type":"journal-article","created":{"date-parts":[[2025,7,26]],"date-time":"2025-07-26T11:48:01Z","timestamp":1753530481000},"source":"Crossref","is-referenced-by-count":3,"title":["Genomic and virulence insights of Western European\n                    <i>Aeromonas salmonicida<\/i>\n                    subsp.\n                    <i>salmonicida<\/i>\n                    and development of\n                    <i>Galleria mellonella<\/i>\n                    infection assay"],"prefix":"10.1093","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4024-1936","authenticated-orcid":false,"given":"Salom\u00e9","family":"Desmecht","sequence":"first","affiliation":[{"name":"University of Li\u00e8ge Veterinary Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, , Avenue de Cureghem,Li\u00e8ge, 4000 ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Touzain","sequence":"additional","affiliation":[{"name":"Viral Genetics and Biosafety Unit, Ploufragan-Plouzan\u00e9-Niort Laboratory, ANSES , Rue des Fusill\u00e9s, Ploufragan, 22440 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibaut","family":"Olivier","sequence":"additional","affiliation":[{"name":"University of Li\u00e8ge Department of Pathology, Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, , Avenue de Cureghem, Li\u00e8ge, 4000 ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8845-3789","authenticated-orcid":false,"given":"C\u00e9line","family":"Antoine","sequence":"additional","affiliation":[{"name":"University of Li\u00e8ge Veterinary Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, , Avenue de Cureghem,Li\u00e8ge, 4000 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