{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:58:42Z","timestamp":1793642322987,"version":"4.5.1"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Medcom Advance S.A"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Rapid detection of bloodstream infection pathogens is essential, as traditional blood cultures require 24\u201372 h, delaying treatment and reducing survival rates. This study aims to develop a simple, efficient strategy for rapidly isolating Pseudomonas aeruginosa (Gram negative), Staphylococcus aureus (Gram positive), and Candida albicans (yeast) directly from blood while preserving whole-cell integrity for downstream applications such as fluorescence staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>The method employs antibody-conjugated magnetic beads (MBs) to capture pathogens directly from blood, enhancing detection sensitivity for low-abundance organisms. Pathogens are eluted as intact cells, enabling visualization via fluorescence staining. The approach successfully isolated P. aeruginosa, S. aureus, and C. albicans, demonstrating robust performance and clinical potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Antibody-conjugated magnetic beads offer a rapid, sensitive, and efficient solution for pathogen isolation from blood, maintaining cell integrity for further analysis. This method improves detection and diagnosis of BSIs, supporting timely interventions and better patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf215","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T11:35:59Z","timestamp":1755948959000},"source":"Crossref","is-referenced-by-count":3,"title":["Isolation of bacteria and yeast from blood using antibody-conjugated magnetic beads"],"prefix":"10.1093","volume":"136","author":[{"given":"Manuel","family":"Garc\u00eda-Algar","sequence":"first","affiliation":[{"name":"Medcom Advance , Carrer de Marcel\u00b7l\u00ed Domingo 2-4, Edifici N5, 43007 Tarragona ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Mur","sequence":"additional","affiliation":[{"name":"Medcom Advance , Carrer de Marcel\u00b7l\u00ed Domingo 2-4, Edifici N5, 43007 Tarragona 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