{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:08:23Z","timestamp":1758845303292,"version":"3.44.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004909","name":"Universidade Federal do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004909","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>This study proposed an in-house in vitro model to investigate the effects of two prebiotic treatments on the gastrointestinal microbiota of piglets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and Results<\/jats:title>\n                  <jats:p>The model involved suspending piglet feces in a culture medium to simulate the ileum and proximal colon regions of the swine gastrointestinal tract. The prebiotics tested were mannanoligosaccharides (MOS) and sodium butyrate. Metabarcoding and culturomics were used to assess the impact of prebiotics on bacterial species composition. Minimum inhibitory concentration tests were conducted to examine bacterial susceptibility patterns. Key bacterial phyla identified included firmicutes, proteobacteria, bacteroidetes, and actinobacteria. Culturomics detected families and several species not identified by metabarcoding. In the simulated proximal colon, MOS increased the abundance of certain species and reduced bacteria with type I fimbriae. Butyric acid promoted beneficial host-associated bacteria and decreased pathogenic species. However, the prebiotics did not significantly affect bacterial susceptibility to antibiotics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The in-house model successfully mimicked piglet intestinal microbiota, allowing for detailed analysis. Both prebiotics positively influenced the piglets microbiota, providing insights into how these treatments potentially influenced the microbiota.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf216","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T15:32:27Z","timestamp":1756481547000},"source":"Crossref","is-referenced-by-count":0,"title":["Successful establishment of a model of the piglet gastrointestinal microbiota and its\u2019 modulation by prebiotics"],"prefix":"10.1093","volume":"136","author":[{"given":"Bruno P","family":"Milnitsky","sequence":"first","affiliation":[{"name":"Institute of Basic Health Science, Universidade Federal do Rio Grande do Sul Microbiology, Immunology and Parasitology Department, , Porto Alegre, RS 90035-003 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry L P","family":"Junior","sequence":"additional","affiliation":[{"name":"ProspectaBio , Porto Alegre, RS 90619-900 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9440-3227","authenticated-orcid":false,"given":"Beni J M","family":"Cha\u00faque","sequence":"additional","affiliation":[{"name":"Postdoctoral fellow at Master\u2019s Program in Clinical Research (MPPC) at the Hospital de Cl\u00ednicas de Porto Alegre (HCPA) (CAPES Pilot Program) , Rio Grande do Sul 90035-903 ,","place":["Brazil"]},{"name":"Postgraduate Program in Biological Sciences: Pharmacology and Therapeutics, UFRGS , Rio Grande do Sul 90035-003 ,","place":["Brazil"]},{"name":"Center of Studies in Science and Technology (NECET), Biology Course, Universidade Rovuma, Niassa Branch , Nangala University Campus, Lichinga ,","place":["Mozambique"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gertrudes","family":"Cor\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Institute of Basic Health Science, Universidade Federal do Rio Grande do Sul Microbiology, Immunology and Parasitology Department, , Porto Alegre, RS 90035-003 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"2025092503513361000_bib1","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1099\/ijs.0.63867-0","article-title":"Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov","volume":"57","author":"Ahmed","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"2025092503513361000_bib2","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1017\/S0043933916000210","article-title":"Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity","volume":"72","author":"Ahsan","year":"2016","journal-title":"World's Poult Sci J"},{"key":"2025092503513361000_bib3","doi-asserted-by":"publisher","first-page":"6446","DOI":"10.1128\/IAI.71.11.6446-6452.2003","article-title":"Type 1 fimbriae of Salmonella enterica serovar Typhimurium bind to enterocytes and contribute to colonization of swine in vivo","volume":"71","author":"Althouse","year":"2003","journal-title":"Infect Immun"},{"article-title":"FastQC: a quality control tool for high throughput sequence data [Online]","year":"2010","author":"Andrews","key":"2025092503513361000_bib4"},{"key":"2025092503513361000_bib5","doi-asserted-by":"publisher","first-page":"1881","DOI":"10.1111\/jam.14069","article-title":"Bifidobacterium animalis ssp. lactis Bb12 induces IL-10 through cell membrane-associated components via TLR2 in swine","volume":"125","author":"Arenas-Padilla","year":"2018","journal-title":"J Appl Microbiol"},{"key":"2025092503513361000_bib58_771_115225","doi-asserted-by":"publisher","first-page":"533","DOI":"10.3389\/fmicb.2017.00533","article-title":"Evaluation of the Probiotic Strain Bifidobacterium longum subsp. 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