{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:26:09Z","timestamp":1791419169778,"version":"4.3.3"},"reference-count":125,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T008695\/1"],"award-info":[{"award-number":["BB\/T008695\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Bioscience for Sustainable Consumer Products","award":["BB\/W009447\/1"],"award-info":[{"award-number":["BB\/W009447\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Chelating agents are metal-sequestering compounds with antibacterial properties suitable for commercial and therapeutic applications. This study investigated the involvement of metal restriction and membrane disruption in the antibacterial mode of action of three chelators.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The antibacterial, metal sequestration, and membrane disruptive effects of ethylenediaminetetraacetic acid, diethylenetriamine pentamethylene phosphonic acid, and fusaric acid were examined across five bacterial species. ICP-MS was used to determine the impact on bacterial metal composition, while RT-qPCR of selected genes allowed evaluation of changes in cellular responses to intracellular metal depletion. Mutants defective in metal import and export machinery were also examined to validate processes critical for resistance. Chelator-mediated disruption of membranes was investigated using 1-N-phenylnapthylamine and propidium iodide. Finally, the capacity of two of the chelators to potentiate the activity of ampicillin, chloramphenicol, tetracycline, and three aminoglycosides was assessed in chequerboards. The results show that these chelators restrict access to iron, zinc, manganese, and calcium to varying degrees in these bacterial species, reflecting important differences in envelope architectures and metal handling capabilities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study shows that all three chelators behave differently in restricting metal access and possess antibacterial properties that often act synergistically in combination, notably with other antimicrobials.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxaf258","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:10:17Z","timestamp":1760530217000},"source":"Crossref","is-referenced-by-count":1,"title":["Antibacterial chelating agents with applications in industry and medicine: cellular metal restriction, membrane disruption, and synergism with antibiotics in\n                    <i>Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Bacillus subtilis<\/i>\n                    , and\n                    <i>Staphylococcus aureus<\/i>"],"prefix":"10.1093","volume":"136","author":[{"given":"Joy R","family":"Paterson","sequence":"first","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany L","family":"Hardman","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren A","family":"Jordan","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M","family":"Wadsworth","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic J","family":"Black","sequence":"additional","affiliation":[{"name":"Durham University Department of Chemistry, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Ross","sequence":"additional","affiliation":[{"name":"Durham University Department of Chemistry, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A","family":"Clare","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Wright","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R","family":"Wallace","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Pal","sequence":"additional","affiliation":[{"name":"Durham University Department of Chemistry, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary T","family":"Moran","sequence":"additional","affiliation":[{"name":"Procter and Gamble Technical Centres , Basingstoke Road, Reading, Berkshire RG2 0RX ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2495-0062","authenticated-orcid":false,"given":"Gary J","family":"Sharples","sequence":"additional","affiliation":[{"name":"Durham University Department of Biosciences, , Stockton Road, Durham DH1 3LE ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"2025120123221354100_bib1","doi-asserted-by":"publisher","first-page":"4695","DOI":"10.1128\/AEM.00142-06","article-title":"Weakening effect of cell permeabilizers on Gram-negative bacteria causing biodeterioration","volume":"72","author":"Alakomi","year":"2006","journal-title":"Appl Environ Microb"},{"key":"2025120123221354100_bib2","doi-asserted-by":"publisher","first-page":"2015","DOI":"10.1099\/mic.0.26312-0","article-title":"Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release","volume":"149","author":"Alakomi","year":"2003","journal-title":"Microbiology"},{"key":"2025120123221354100_bib3","doi-asserted-by":"publisher","first-page":"e02353","DOI":"10.1128\/AAC.02353-19","article-title":"Iron sequestrant DIBI, a potential alternative for nares decolonization of methicillin-resistant Staphylococcus aureus, is anti-infective and inhibitory for mupirocin-resistant isolates","volume":"64","author":"Allan","year":"2020","journal-title":"Antimicrob Agents Chemother"},{"key":"2025120123221354100_bib4","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1016\/j.micinf.2006.01.022","article-title":"Transcriptional modulation of some Staphylococcus aureus iron-regulated genes during growth in vitro and in a tissue cage model in vivo","volume":"8","author":"Allard","year":"2006","journal-title":"Microbes Infect"},{"key":"2025120123221354100_bib5","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1128\/jb.81.5.741-746.1961","article-title":"Requirements for transformation in Bacillus subtilis","volume":"81","author":"Anagnostopoulos","year":"1961","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib6","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1093\/jac\/48.suppl_1.5","article-title":"Determination of minimum inhibitory concentrations","volume":"48","author":"Andrews","year":"2001","journal-title":"J Antimicrob Chemother"},{"key":"2025120123221354100_bib7","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1111\/j.1365-2958.2009.06699.x","article-title":"Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli","volume":"72","author":"Anjem","year":"2009","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib8","doi-asserted-by":"publisher","first-page":"2006.0008","DOI":"10.1038\/msb4100050","article-title":"Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection","volume":"2","author":"Baba","year":"2006","journal-title":"Mol Syst Biol"},{"key":"2025120123221354100_bib9","doi-asserted-by":"publisher","first-page":"4039","DOI":"10.1128\/aem.62.11.4039-4043.1996","article-title":"Production of fusaric acid by Fusarium species","volume":"62","author":"Bacon","year":"1996","journal-title":"Appl Environ Microb"},{"key":"2025120123221354100_bib10","doi-asserted-by":"publisher","first-page":"1855","DOI":"10.1021\/acsinfecdis.0c00102","article-title":"Kanamycin uptake into Escherichia coli is facilitated by OmpF and OmpC porin channels located in the outer membrane","volume":"6","author":"Bafna","year":"2020","journal-title":"ACS Infect Dis"},{"key":"2025120123221354100_bib11","doi-asserted-by":"publisher","first-page":"102103","DOI":"10.1016\/j.cbpa.2021.102103","article-title":"Metallo-beta-lactamases and a tug-of-war for the available zinc at the host-pathogen interface","volume":"66","author":"Bahr","year":"2022","journal-title":"Curr Opin Chem Biol"},{"key":"2025120123221354100_bib12","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/S0732-8893(02)00473-X","article-title":"Comparison of methods of interpretation of checkerboard synergy testing","volume":"44","author":"Bonapace","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2025120123221354100_bib13","doi-asserted-by":"publisher","first-page":"fuab028","DOI":"10.1093\/femsre\/fuab028","article-title":"Regulation and distinct physiological roles of manganese in bacteria","volume":"45","author":"Bosma","year":"2021","journal-title":"FEMS Microbiol Rev"},{"key":"2025120123221354100_bib14","doi-asserted-by":"publisher","first-page":"12350","DOI":"10.1016\/S0021-9258(19)49846-3","article-title":"Overexpression and purification of ferric enterobactin esterase from Escherichia coli: demonstration of enzymatic hydrolysis of enterobactin and its iron complex","volume":"267","author":"Brickman","year":"1992","journal-title":"J Biol Chem"},{"key":"2025120123221354100_bib15","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1046\/j.1365-2958.1999.01229.x","article-title":"ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli","volume":"31","author":"Brocklehurst","year":"1999","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib16","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1093\/clinids\/11.3.361","article-title":"Inoculum effect","volume":"11","author":"Brook","year":"1989","journal-title":"Clin Infect Dis"},{"key":"2025120123221354100_bib17","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1099\/00221287-59-2-263","article-title":"Role of divalent cations in the action of polymyxin B and EDTA on Pseudomonas aeruginosa","volume":"59","author":"Brown","year":"1969","journal-title":"J Gen Microbiol"},{"key":"2025120123221354100_bib18","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1038\/2071391a0","article-title":"Effect of ethylenediamine tetraacetate on the resistance of Pseudomonas aeruginosa to antibacterial agents","volume":"207","author":"Brown","year":"1965","journal-title":"Nature"},{"key":"2025120123221354100_bib19","doi-asserted-by":"publisher","first-page":"943390","DOI":"10.3389\/fcimb.2023.943390","article-title":"Why is manganese so valuable to bacterial pathogens?","volume":"13","author":"Capek","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"key":"2025120123221354100_bib20","doi-asserted-by":"publisher","first-page":"4065","DOI":"10.1039\/C7NJ00118E","article-title":"On the complexation of metal cations with \u201cpure\u201d diethylenetriamine-N,N,N\u2032,N\u2032,N\u2032-pentakis(methylenephosphonic) acid","volume":"41","author":"Cardiano","year":"2017","journal-title":"New J Chem"},{"key":"2025120123221354100_bib21","doi-asserted-by":"publisher","first-page":"e01909","DOI":"10.1128\/AAC.01909-19","article-title":"Forging new antibiotic combinations under iron-limiting conditions","volume":"64","author":"Chan","year":"2020","journal-title":"Antimicrob Agents Chemother"},{"key":"2025120123221354100_bib22","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1038\/nrmicro.2017.15","article-title":"Metal homeostasis and resistance in bacteria","volume":"15","author":"Chandrangsu","year":"2017","journal-title":"Nat Rev Micro"},{"key":"2025120123221354100_bib23","doi-asserted-by":"publisher","first-page":"6762","DOI":"10.1093\/nar\/gkm631","article-title":"Discovery of Fur binding site clusters in Escherichia coli by information theory models","volume":"35","author":"Chen","year":"2007","journal-title":"Nucl Acids Res"},{"key":"2025120123221354100_bib24","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1007\/s10886-017-0889-x","article-title":"Microbial resistance mechanisms to the antibiotic and phytotoxin fusaric acid","volume":"43","author":"Crutcher","year":"2017","journal-title":"J Chem Ecol"},{"key":"2025120123221354100_bib25","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1104\/pp.74.1.39","article-title":"Effects of fusaric acid on tomato root hair membrane potentials and ATP levels","volume":"74","author":"D\u2019Alton","year":"1984","journal-title":"Plant Physiol"},{"key":"2025120123221354100_bib26","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1128\/AAC.7.5.596","article-title":"Effect of calcium and magnesium ions on the susceptibility of Pseudomonas species to tetracycline, gentamicin polymyxin B, and carbenicillin","volume":"7","author":"D\u2019Amato","year":"1975","journal-title":"Antimicrob Agents Chemother"},{"key":"2025120123221354100_bib27","doi-asserted-by":"publisher","first-page":"3841","DOI":"10.1073\/pnas.1220341110","article-title":"Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens","volume":"110","author":"Damo","year":"2013","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025120123221354100_bib28","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1016\/j.bbapap.2008.11.005","article-title":"Outer membrane permeability and antibiotic resistance","volume":"1794","author":"Delcour","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"2025120123221354100_bib29","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1046\/j.1469-0691.2000.00142.x","article-title":"Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution","volume":"6","author":"European Committee for Antimicrobial Susceptibility Testing. T.","year":"2000","journal-title":"Clin Microbiol Infect"},{"key":"2025120123221354100_bib30","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.3390\/microorganisms9071434","article-title":"Functional redundancy and specialization of the conserved cold shock proteins in Bacillus subtilis","volume":"9","author":"Fasshauer","year":"2021","journal-title":"Microorganisms"},{"key":"2025120123221354100_bib31","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1128\/JCM.02464-08","article-title":"High concentrations of manganese in Mueller-Hinton agar increase MICs of tigecycline determined by Etest","volume":"47","author":"Fernandez-Mazarrasa","year":"2009","journal-title":"J Clin Microbiol"},{"key":"2025120123221354100_bib32","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1089\/wound.2014.0577","article-title":"EDTA: an antimicrobial and antibiofilm agent for use in wound care","volume":"4","author":"Finnegan","year":"2015","journal-title":"Adv Wound Care"},{"key":"2025120123221354100_bib33","doi-asserted-by":"publisher","first-page":"mfac058","DOI":"10.1093\/mtomcs\/mfac058","article-title":"Metalation calculators for E. coli strain JM109 (DE3): aerobic, anaerobic, and hydrogen peroxide exposed cells cultured in LB media","volume":"14","author":"Foster","year":"2022","journal-title":"Metallomics"},{"key":"2025120123221354100_bib34","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1038\/s41570-023-00463-4","article-title":"Metals to combat antimicrobial resistance","volume":"7","author":"Frei","year":"2023","journal-title":"Nat Rev Chem"},{"key":"2025120123221354100_bib35","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1023\/A:1023425321617","article-title":"Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems","volume":"16","author":"Gaballa","year":"2003","journal-title":"Biometals"},{"key":"2025120123221354100_bib36","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1126\/science.aaf1018","article-title":"Biosynthesis of a broad-spectrum nicotianamine-like metallophore in Staphylococcus aureus","volume":"352","author":"Ghssein","year":"2016","journal-title":"Science"},{"key":"2025120123221354100_bib37","doi-asserted-by":"publisher","first-page":"S37","DOI":"10.1093\/infdis\/124.Supplement_1.S37","article-title":"Effect of the concentrations of magnesium and calcium on the in-vitro susceptibility of Pseudomonas aeruginosa to gentamicin","volume":"124","author":"Gilbert","year":"1971","journal-title":"J Infect Dis"},{"key":"2025120123221354100_bib38","doi-asserted-by":"publisher","first-page":"2414","DOI":"10.1128\/JCM.06686-11","article-title":"Cation concentration variability of four distinct Mueller-Hinton agar brands influences polymyxin B susceptibility results","volume":"50","author":"Girardello","year":"2012","journal-title":"J Clin Microbiol"},{"key":"2025120123221354100_bib39","doi-asserted-by":"publisher","first-page":"29406","DOI":"10.1074\/jbc.M110.147579","article-title":"Crystal structure of Staphylococcus aureus metallopeptidase (Sapep) reveals large domain motions between the manganese-bound and apo-states","volume":"285","author":"Girish","year":"2010","journal-title":"J Biol Chem"},{"key":"2025120123221354100_bib40","doi-asserted-by":"publisher","first-page":"1604","DOI":"10.1128\/JB.187.5.1604-1611.2005","article-title":"The metal permease ZupT from Escherichia coli is a transporter with a broad substrate spectrum","volume":"187","author":"Grass","year":"2005","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib41","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.jinorgbio.2009.09.012","article-title":"Structural biology of heme binding in the Staphylococcus aureus Isd system","volume":"104","author":"Grigg","year":"2010","journal-title":"J Inorg Biochem"},{"key":"2025120123221354100_bib42","doi-asserted-by":"publisher","first-page":"e01281","DOI":"10.1128\/mBio.01281-17","article-title":"The metallophore staphylopineenables Staphylococcus aureus to compete with the host for zinc and overcome nutritional immunity","volume":"8","author":"Grim","year":"2017","journal-title":"mBio"},{"key":"2025120123221354100_bib43","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.jmb.2006.07.057","article-title":"Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose","volume":"363","author":"Grizot","year":"2006","journal-title":"J Mol Biol"},{"key":"2025120123221354100_bib44","doi-asserted-by":"publisher","first-page":"19668","DOI":"10.1021\/jacs.0c09352","article-title":"Biosynthesis of mycotoxin fusaric acid and application of a PLP-dependent enzyme for chemoenzymatic synthesis of substituted L-pipecolic acids","volume":"142","author":"Hai","year":"2020","journal-title":"J Am Chem Soc"},{"key":"2025120123221354100_bib45","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1146\/annurev-micro-090110-102851","article-title":"Molecular mechanisms of Staphylococcus aureus iron acquisition","volume":"65","author":"Hammer","year":"2011","journal-title":"Annu Rev Microbiol"},{"key":"2025120123221354100_bib46","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1093\/jac\/8.6.429","article-title":"Aminoglycoside uptake and mode of action-with special reference to streptomycin and gentamicin. II. Effects of aminoglycosides on cells","volume":"8","author":"Hancock","year":"1981","journal-title":"J Antimicrob Chemother"},{"key":"2025120123221354100_bib47","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1146\/annurev.mi.38.100184.001321","article-title":"Alterations in outer membrane permeability","volume":"38","author":"Hancock","year":"1984","journal-title":"Annu Rev Microbiol"},{"key":"2025120123221354100_bib48","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0966-842X(97)81773-8","article-title":"The bacterial outer membrane as a drug barrier","volume":"5","author":"Hancock","year":"1997","journal-title":"Trends Microbiol"},{"key":"2025120123221354100_bib49","doi-asserted-by":"publisher","first-page":"1431","DOI":"10.1080\/15476286.2017.1300223","article-title":"Both exo- and endo-nucleolytic activities of RNase J1 from Staphylococcus aureus are manganese dependent and active on triphosphorylated 5'-ends","volume":"14","author":"Hausmann","year":"2017","journal-title":"RNA Biology"},{"key":"2025120123221354100_bib50","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1046\/j.1365-2672.2000.00971.x","article-title":"Fluorometric assessment of Gram-negative bacterial permeabilization","volume":"88","author":"Helander","year":"2000","journal-title":"J Appl Microbiol"},{"key":"2025120123221354100_bib51","doi-asserted-by":"publisher","first-page":"28112","DOI":"10.1074\/jbc.R114.587071","article-title":"Specificity of metal sensing: iron and manganese homeostasis in Bacillus subtilis","volume":"289","author":"Helmann","year":"2014","journal-title":"J Biol Chem"},{"key":"2025120123221354100_bib52","doi-asserted-by":"publisher","first-page":"1371","DOI":"10.1128\/AAC.47.4.1371-1375.2003","article-title":"MexXY-OprM efflux pump is necessary for a adaptive resistance of Pseudomonas aeruginosa to aminoglycosides","volume":"47","author":"Hocquet","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"2025120123221354100_bib53","doi-asserted-by":"publisher","first-page":"3744","DOI":"10.1128\/IAI.69.6.3744-3754.2001","article-title":"PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in Staphylococcus aureus","volume":"69","author":"Horsburgh","year":"2001","journal-title":"Infect Immun"},{"key":"2025120123221354100_bib54","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1046\/j.1365-2958.2002.02944.x","article-title":"MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake","volume":"44","author":"Horsburgh","year":"2002","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib55","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1099\/00221287-137-4-725","article-title":"Metal speciation and microbial growth\u2014the hard (and soft) facts","volume":"137","author":"Hughes","year":"1991","journal-title":"J Gen Microbiol"},{"key":"2025120123221354100_bib56","doi-asserted-by":"publisher","first-page":"427","DOI":"10.3389\/fmicb.2018.00427","article-title":"Parallel evolution of high-level aminoglycoside resistance in Escherichia coli under low and high mutation supply rates","volume":"9","author":"Ibacache-Quiroga","year":"2018","journal-title":"Front Microbiol"},{"key":"2025120123221354100_bib57","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.jff.2016.10.027","article-title":"Effect of culture media on chemical stability and antibacterial activity of allicin","volume":"28","author":"Imani\u00a0Rad","year":"2017","journal-title":"J Funct Foods"},{"key":"2025120123221354100_bib58","doi-asserted-by":"publisher","first-page":"28121","DOI":"10.1074\/jbc.R114.588814","article-title":"The mismetallation of enzymes during oxidative stress","volume":"289","author":"Imlay","year":"2014","journal-title":"J Biol Chem"},{"key":"2025120123221354100_bib59","doi-asserted-by":"publisher","first-page":"5125","DOI":"10.1038\/s41467-024-49218-9","article-title":"Fusaric acid mediates the assembly of disease-suppressive rhizosphere microbiota via induced shifts in plant root exudates","volume":"15","author":"Jin","year":"2024","journal-title":"Nat Commun"},{"key":"2025120123221354100_bib60","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1038\/s44259-024-00051-6","article-title":"Antibiotic susceptibility testing using minimum inhibitory concentration (MIC) assays","volume":"2","author":"Kaderabkova","year":"2024","journal-title":"Npj Antimicrob Resist"},{"key":"2025120123221354100_bib61","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat Rev Micro"},{"key":"2025120123221354100_bib62","doi-asserted-by":"publisher","first-page":"3395","DOI":"10.1128\/IAI.00420-13","article-title":"MntABC and MntH contribute to systemic Staphylococcus aureus infection by competing with calprotectin for nutrient manganese","volume":"81","author":"Kehl-Fie","year":"2013","journal-title":"Infect Immun"},{"key":"2025120123221354100_bib63","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/S0165-9936(03)01008-2","article-title":"Synthetic chelating agents and compounds exhibiting complexing properties in the aquatic environment","volume":"22","author":"Knepper","year":"2003","journal-title":"TrAC, Trends Anal Chem"},{"key":"2025120123221354100_bib64","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1093\/jac\/46.3.369","article-title":"Comparison of cation-adjusted Mueller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method","volume":"46","author":"Koeth","year":"2000","journal-title":"J Antimicrob Chemother"},{"key":"2025120123221354100_bib65","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.cels.2016.12.013","article-title":"Construction and analysis of two genome-scale deletion libraries for Bacillus subtilis","volume":"4","author":"Koo","year":"2017","journal-title":"Cell Syst"},{"key":"2025120123221354100_bib66","doi-asserted-by":"publisher","first-page":"a027029","DOI":"10.1101\/cshperspect.a027029","article-title":"Aminoglycosides: an overview","volume":"6","author":"Krause","year":"2016","journal-title":"Cold Spring Harb Perspect Med"},{"key":"2025120123221354100_bib67","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1046\/j.1365-2672.2004.02135.x","article-title":"The synergistic effect of EDTA\/antimicrobial combinations on Pseudomonas aeruginosa","volume":"96","author":"Lambert","year":"2004","journal-title":"J Appl Microbiol"},{"key":"2025120123221354100_bib68","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1139\/w02-094","article-title":"Effect of fusaric acid and phytoanticipins on growth of rhizobacteria and Fusarium oxysporum","volume":"48","author":"Landa","year":"2002","journal-title":"Can J Microbiol"},{"key":"2025120123221354100_bib69","doi-asserted-by":"publisher","first-page":"e0003622","DOI":"10.1128\/mmbr.00036-22","article-title":"Aminoglycoside uptake, stress, and potentiation in Gram-negative bacteria: new therapies with old molecules","volume":"87","author":"Lang","year":"2023","journal-title":"Microbiol Mol Biol Rev"},{"key":"2025120123221354100_bib70","article-title":"Synergism testing: broth microdilution checkerboard and broth macrodilution methods","volume-title":"Clinical Microbiology Procedures Handbook","author":"Leber","year":"2016"},{"key":"2025120123221354100_bib71","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1016\/0006-291X(65)90191-9","article-title":"Release of lipopolysaccharide by EDTA treatment of E. coli","volume":"21","author":"Leive","year":"1965","journal-title":"Biochem Biophys Res Commun"},{"key":"2025120123221354100_bib72","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1111\/j.1749-6632.1974.tb43261.x","article-title":"The barrier function of the Gram-negative envelope","volume":"235","author":"Leive","year":"1974","journal-title":"Ann NY Acad Sci"},{"key":"2025120123221354100_bib73","doi-asserted-by":"publisher","first-page":"lxae301","DOI":"10.1093\/jambio\/lxae301","article-title":"Disruption of cell wall and membrane integrity as antioomycete and antifungal mode of action by fusaric and 9,10-dehydrofusaric acids from endophytic fungus Fusarium lactis strain SME13\u20132","volume":"136","author":"Macias-Rubalcava","year":"2025","journal-title":"J Appl Microbiol"},{"key":"2025120123221354100_bib74","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1046\/j.1365-2958.2000.01774.x","article-title":"Identification of the Escherichia coli K-12 Nramp orthologue (MntH) as a selective divalent metal ion transporter","volume":"35","author":"Makui","year":"2000","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib75","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.1128\/AAC.45.7.2001-2007.2001","article-title":"MexXY-OprM efflux pump is required for antagonism of aminoglycosides by divalent cations in Pseudomonas aeruginosa","volume":"45","author":"Mao","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"2025120123221354100_bib76","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1006\/pmpp.1993.1012","article-title":"Relationship between fusaric acid uptake and its binding to cell structures by leaves of Egeria densa and its toxic effects on membrane-permeability and respiration","volume":"42","author":"Marre","year":"1993","journal-title":"Physiol Mol Plant Pathol"},{"key":"2025120123221354100_bib77","doi-asserted-by":"publisher","first-page":"6919","DOI":"10.1128\/JB.00904-07","article-title":"Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors","volume":"189","author":"Mascher","year":"2007","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib78","doi-asserted-by":"publisher","first-page":"e01541","DOI":"10.1128\/mBio.01541-16","article-title":"Antibiotic resistance and regulation of the Gram-negative bacterial outer membrane barrier by host innate immune molecules","volume":"7","author":"Miller","year":"2016","journal-title":"mBio"},{"key":"2025120123221354100_bib79","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1038\/s41579-024-01054-w","article-title":"ESKAPE pathogens: antimicrobial resistance, epidemiology, clinical impact and therapeutics","volume":"22","author":"Miller","year":"2024","journal-title":"Nat Rev Micro"},{"key":"2025120123221354100_bib80","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc09c01s28","article-title":"Growth and laboratory maintenance of Staphylococcus aureus","volume":"28:","author":"Missiakas","year":"2013","journal-title":"CP Microbiology"},{"key":"2025120123221354100_bib81","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1038\/s41579-022-00745-6","article-title":"Nutritional immunity: the battle for nutrient metals at the host-pathogen interface","volume":"20","author":"Murdoch","year":"2022","journal-title":"Nat Rev Micro"},{"key":"2025120123221354100_bib82","doi-asserted-by":"publisher","first-page":"e00754","DOI":"10.1128\/JB.00754-18","article-title":"Proteomic analysis of the Pseudomonas aeruginosa iron starvation response reveals PrrF small regulatory RNA-dependent iron regulation of twitching motility, amino acid metabolism, and zinc homeostasis proteins","volume":"201","author":"Nelson","year":"2019","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib83","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1128\/jb.143.2.872-878.1980","article-title":"Outer membrane protein H1 of Pseudomonas aeruginosa: involvement in adaptive and mutational resistance to ethylenediaminetetraacetate, polymyxin B, and gentamicin","volume":"143","author":"Nicas","year":"1980","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib84","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/bk-2005-0910.ch001","article-title":"Chelating agents in the environment","volume-title":"Biogeochemistry of Chelating Agents","author":"Nowack","year":"2005"},{"key":"2025120123221354100_bib85","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1039\/dt9840001133","article-title":"Spectroscopic studies of transition-metal ion complexes of diethylenetriaminepenta-acetic acid and diethylenetriaminepenta-methylphosphonic acid","author":"Oakes","year":"1984","journal-title":"J Chem Soc, Dalton Trans"},{"key":"2025120123221354100_bib86","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1046\/j.1365-2958.2002.03084.x","article-title":"GeneChip expression analysis of the iron starvation response in Pseudomonas aeruginosa: identification of novel pyoverdine biosynthesis genes","volume":"45","author":"Ochsner","year":"2002","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib87","doi-asserted-by":"publisher","first-page":"3664","DOI":"10.1128\/JB.188.10.3664-3673.2006","article-title":"Role of the Fur regulon in iron transport in Bacillus subtilis","volume":"188","author":"Ollinger","year":"2006","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib88","doi-asserted-by":"publisher","first-page":"9696","DOI":"10.1038\/s41598-019-46100-3","article-title":"Pleiotropic effects of rfa-gene mutations on Escherichia coli envelope properties","volume":"9","author":"Pagnout","year":"2019","journal-title":"Sci Rep"},{"key":"2025120123221354100_bib89","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.3389\/fmicb.2018.01811","article-title":"Novel iron-chelator DIBI inhibits Staphylococcus aureus growth, suppresses experimental MRSA infection in mice and enhances the activities of diverse antibiotics in vitro","volume":"9","author":"Parquet","year":"2018","journal-title":"Front Microbiol"},{"key":"2025120123221354100_bib90","doi-asserted-by":"publisher","first-page":"e0164121","DOI":"10.1128\/AEM.01641-21","article-title":"Insights into the antibacterial mechanism of action of chelating agents by selective deprivation of iron, manganese, and zinc","volume":"88","author":"Paterson","year":"2022","journal-title":"Appl Environ Microb"},{"key":"2025120123221354100_bib91","first-page":"001153","article-title":"A critical role for iron and zinc homeostatic systems in the evolutionary adaptation of Escherichia coli to metal restriction","volume":"9","author":"Paterson","year":"2023","journal-title":"Microbiol Genom"},{"key":"2025120123221354100_bib92","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/s44259-025-00131-1","article-title":"Enhanced resistance of metal sequestering agents by reconfiguration of the Staphylococcus aureus cell wall","volume":"3","author":"Paterson","year":"2025","journal-title":"Npj Antimicrob Resist"},{"key":"2025120123221354100_bib93","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1046\/j.1365-2958.1998.00883.x","article-title":"The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli","volume":"28","author":"Patzer","year":"1998","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib94","doi-asserted-by":"publisher","first-page":"840","DOI":"10.1039\/C7MT00112F","article-title":"Ferrous iron efflux systems in bacteria","volume":"9","author":"Pi","year":"2017","journal-title":"Metallomics"},{"key":"2025120123221354100_bib95","doi-asserted-by":"publisher","first-page":"11893","DOI":"10.1007\/s11356-014-2592-6","article-title":"Biodegradable chelating agents for industrial, domestic, and agricultural applications\u2014a review","volume":"21","author":"Pinto","year":"2014","journal-title":"Environ Sci Pollut Res"},{"key":"2025120123221354100_bib96","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1111\/j.1745-7270.2007.00350.x","article-title":"EDTA-induced membrane fluidization and destabilization: biophysical studies on artificial lipid membranes","volume":"39","author":"Prachayasittikul","year":"2007","journal-title":"ABBS"},{"key":"2025120123221354100_bib97","doi-asserted-by":"publisher","first-page":"e00642","DOI":"10.1128\/IAI.00642-18","article-title":"Synergy between nutritional immunity and independent host defenses contributes to the importance of the MntABC manganese transporter during Staphylococcus aureus infection","volume":"87","author":"Radin","year":"2019","journal-title":"Infect Immun"},{"key":"2025120123221354100_bib98","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1146\/annurev.biochem.71.110601.135414","article-title":"Lipopolysaccharide endotoxins","volume":"71","author":"Raetz","year":"2002","journal-title":"Annu Rev Biochem"},{"key":"2025120123221354100_bib99","doi-asserted-by":"publisher","first-page":"14326","DOI":"10.1073\/pnas.94.26.14326","article-title":"The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase","volume":"94","author":"Rensing","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025120123221354100_bib100","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1016\/j.scitotenv.2017.09.223","article-title":"Organophosphonates: a review on environmental relevance, biodegradability and removal in wastewater treatment plants","volume":"615","author":"Rott","year":"2018","journal-title":"Sci Total Environ"},{"key":"2025120123221354100_bib101","doi-asserted-by":"publisher","first-page":"e0117040","DOI":"10.1371\/journal.pone.0117040","article-title":"Production of siderophores increases resistance to fusaric acid in Pseudomonas protegens Pf-5","volume":"10","author":"Ruiz","year":"2015","journal-title":"PLoS One"},{"key":"2025120123221354100_bib102","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1111\/1751-7915.14241","article-title":"Mechanisms of iron homeostasis in Pseudomonas aeruginosa and emerging therapeutics directed to disrupt this vital process","volume":"16","author":"Sanchez-Jimenez","year":"2023","journal-title":"Microb Biotechnol"},{"key":"2025120123221354100_bib103","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1111\/1462-2920.16328","article-title":"Pseudomonas aeruginosa and its multiple strategies to access iron","volume":"25","author":"Schalk","year":"2023","journal-title":"Environ Microbiol"},{"key":"2025120123221354100_bib104","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/s41579-024-01090-6","article-title":"Bacterial siderophores: diversity, uptake pathways and applications","volume":"23","author":"Schalk","year":"2025","journal-title":"Nat Rev Micro"},{"key":"2025120123221354100_bib105","doi-asserted-by":"publisher","first-page":"8746","DOI":"10.1128\/JB.01368-07","article-title":"Escherichia coli physiology in Luria-Bertani broth","volume":"189","author":"Sezonov","year":"2007","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib106","doi-asserted-by":"publisher","first-page":"21676","DOI":"10.1038\/s41598-021-01052-5","article-title":"Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections","volume":"11","author":"Shein","year":"2021","journal-title":"Sci Rep"},{"key":"2025120123221354100_bib107","doi-asserted-by":"publisher","first-page":"001289","DOI":"10.1099\/mic.0.001289","article-title":"Iron homeostasis in Bacillus subtilis relies on three differentially expressed efflux systems","volume":"169","author":"Steingard","year":"2023","journal-title":"Microbiology (Reading)"},{"key":"2025120123221354100_bib108","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/s12866-015-0376-x","article-title":"Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide","volume":"15","author":"Stiefel","year":"2015","journal-title":"BMC Microbiol"},{"key":"2025120123221354100_bib109","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1002\/cyto.a.20069","article-title":"Mechanism and use of the commercially available viability stain, BacLight","volume":"61A","author":"Stocks","year":"2004","journal-title":"Cytometry Pt A"},{"key":"2025120123221354100_bib110","doi-asserted-by":"publisher","first-page":"e14399","DOI":"10.7717\/peerj.14399","article-title":"Serratia marcescens antibiotic resistance mechanisms of an opportunistic pathogen: a literature review","volume":"11","author":"Tavares-Carreon","year":"2023","journal-title":"PeerJ"},{"key":"2025120123221354100_bib111","doi-asserted-by":"publisher","first-page":"e1007537","DOI":"10.1371\/journal.ppat.1007537","article-title":"Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM","volume":"15","author":"Tosi","year":"2019","journal-title":"PLoS Pathog"},{"key":"2025120123221354100_bib112","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1128\/mr.56.3.395-411.1992","article-title":"Agents that increase the permeability of the outer membrane","volume":"56","author":"Vaara","year":"1992","journal-title":"Microbiol Rev"},{"key":"2025120123221354100_bib113","doi-asserted-by":"publisher","first-page":"857237","DOI":"10.3389\/fcell.2022.857237","article-title":"The role of iron in Staphylococcus aureus infection and human disease: a metal tug of war at the host-microbe interface","volume":"10","author":"van\u00a0Dijk","year":"2022","journal-title":"Front Cell Dev Biol"},{"key":"2025120123221354100_bib114","doi-asserted-by":"publisher","first-page":"2805","DOI":"10.1099\/mic.0.28063-0","article-title":"Influence of fusaric acid on phenazine-1-carboxamide synthesis and gene expression of Pseudomonas chlororaphis strain PCL1391","volume":"151","author":"van\u00a0Rij","year":"2005","journal-title":"Microbiology (Reading)"},{"key":"2025120123221354100_bib115","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1046\/j.1365-2958.1999.01586.x","article-title":"The response of Pseudomonas aeruginosa to iron: genetics, biochemistry and virulence","volume":"34","author":"Vasil","year":"1999","journal-title":"Mol Microbiol"},{"key":"2025120123221354100_bib116","doi-asserted-by":"publisher","first-page":"S91","DOI":"10.1017\/S0007114500002300","article-title":"Mineral-binding milk proteins and peptides; occurrence, biochemical and technological characteristics","volume":"84","author":"Vegarud","year":"2000","journal-title":"Br J Nutr"},{"key":"2025120123221354100_bib117","doi-asserted-by":"publisher","first-page":"2876","DOI":"10.3390\/ijms22062876","article-title":"Recent advances in iron chelation and gallium-based therapies for antibiotic resistant bacterial infections","volume":"22","author":"Vinuesa","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2025120123221354100_bib118","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1099\/00221287-48-3-391","article-title":"Effects of organic cations on the gram-negative cell wall and their bactericidal activity with ethylenediaminetetra-acetate and surface active agents","volume":"48","author":"Voss","year":"1967","journal-title":"J Gen Microbiol"},{"key":"2025120123221354100_bib119","doi-asserted-by":"publisher","first-page":"5887","DOI":"10.1128\/JB.05872-11","article-title":"The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis","volume":"193","author":"Waters","year":"2011","journal-title":"J Bacteriol"},{"key":"2025120123221354100_bib120","doi-asserted-by":"publisher","first-page":"1365","DOI":"10.1038\/2191365a0","article-title":"In vitro reversal of antibiotic resistance by ethylenediamine tetraacetic acid","volume":"219","author":"Weiser","year":"1968","journal-title":"Nature"},{"key":"2025120123221354100_bib121","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1016\/S0140-6736(69)90328-6","article-title":"Synergistic effect of edetic-acid\/antibiotic combinations on Pseudomonas aeruginosa","volume":"294","author":"Weiser","year":"1969","journal-title":"The Lancet"},{"key":"2025120123221354100_bib122","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances","volume":"3","author":"Wiegand","year":"2008","journal-title":"Nat Protoc"},{"key":"2025120123221354100_bib123","doi-asserted-by":"publisher","first-page":"e0176023","DOI":"10.1128\/mbio.01760-23","article-title":"An exhaustive multiple knockout approach to understanding cell wall hydrolase function in Bacillus subtilis","volume":"14","author":"Wilson","year":"2023","journal-title":"mBio"},{"key":"2025120123221354100_bib124","doi-asserted-by":"publisher","first-page":"1830","DOI":"10.1038\/s41467-021-21942-6","article-title":"Translation error clusters induced by aminoglycoside antibiotics","volume":"12","author":"Wohlgemuth","year":"2021","journal-title":"Nat Commun"},{"key":"2025120123221354100_bib125","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1093\/infdis\/127.6.663","article-title":"Activity of aminoglycoside antibiotics aganst Pseudomonas aeruginosa: specificity and site of calcium and magnesium antagonism","volume":"127","author":"Zimelis","year":"1973","journal-title":"J Infect Dis"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxaf258\/64718412\/lxaf258.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/136\/11\/lxaf258\/64718412\/lxaf258.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/136\/11\/lxaf258\/64718412\/lxaf258.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T04:22:18Z","timestamp":1764649338000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxaf258\/8287734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,16]]},"references-count":125,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,4]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxaf258","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,11]]},"published":{"date-parts":[[2025,10,16]]},"article-number":"lxaf258"}}