{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:09:02Z","timestamp":1785510542645,"version":"3.56.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intestinal colonization by multidrug-resistant Enterobacteriaceae represents a major public health concern as it can lead to difficult-to-treat infections, environmental contamination, and transmission. This study evaluated the efficacy of a prophylactic treatment using Escherichia coli strains isolated from murine feces [commensal E. coli (comEc)] to reduce intestinal colonization by extended-spectrum beta-lactamase (ESBL)-producing E. coli in a murine model of antibiotic-induced dysbiosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The comEc treatment was administered intragastrically 1 day prior to ESBL-producing E. coli challenge. Colonization levels were monitored daily using culture-based quantification. Fecal microbiota composition was analyzed before the ESBL-producing E. coli challenge to identify predictors of treatment efficacy. The comEc treatment significantly reduced mean ESBL-producing E. coli levels during the first four days. Notably, 14% of treated mice exhibited marked and sustained decolonization, whereas others had only a limited and transient effect.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this proof-of-concept study, prophylactic treatment with murine-derived E. coli strains transiently reduced fecal ESBL-producing E. coli titers, although pronounced efficacy was observed in a small subset of animals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag041","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T12:48:47Z","timestamp":1770382127000},"source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of\n                    <i>Escherichia coli<\/i>\n                    as a prophylactic treatment of intestinal colonization by extended-spectrum beta-lactamase-producing\n                    <i>E. coli<\/i>\n                    in a murine model of antibiotic-induced dysbiosis"],"prefix":"10.1093","volume":"137","author":[{"given":"Murad","family":"Ishnaiwer","sequence":"first","affiliation":[{"name":"Palestine Polytechnic University (PPU) , Hebron, Palestine, PO BOX: 198"},{"name":"UR1155 Cibles et m\u00e9dicaments des infections et de l\u2019immunit\u00e9 (IICiMed), Nantes Universit\u00e9 , 44200, Nantes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Dion","sequence":"additional","affiliation":[{"name":"UR1155 Cibles et m\u00e9dicaments des infections et de l\u2019immunit\u00e9 (IICiMed), Nantes Universit\u00e9 , 44200, Nantes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Montassier","sequence":"additional","affiliation":[{"name":"CHU Nantes Emergency Department, , 44200, Nantes ,","place":["France"]},{"name":"Inserm, CHU Nantes, Center for Research in Transplantation and Translational Immunology, Nantes Universit\u00e9 , UMR 1064, 44093, Nantes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quentin","family":"Le\u00a0Bastard","sequence":"additional","affiliation":[{"name":"UR1155 Cibles et m\u00e9dicaments des infections et de l\u2019immunit\u00e9 (IICiMed), Nantes Universit\u00e9 , 44200, Nantes ,","place":["France"]},{"name":"CHU Nantes Emergency Department, , 44200, Nantes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Batard","sequence":"additional","affiliation":[{"name":"UR1155 Cibles et m\u00e9dicaments des infections et de l\u2019immunit\u00e9 (IICiMed), Nantes Universit\u00e9 , 44200, Nantes ,","place":["France"]},{"name":"CHU Nantes Emergency Department, , 44200, Nantes ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"2026051508375280000_bib1","doi-asserted-by":"publisher","first-page":"2291","DOI":"10.19082\/2291","article-title":"Molecular typing of uropathogenic Escherichia coli strains by the ERIC-PCR method","volume":"8","author":"Afkhami\u00a0Ardakani","year":"2016","journal-title":"Electron Physician"},{"key":"2026051508375280000_bib46_245_173526","doi-asserted-by":"publisher","first-page":"1981","DOI":"10.3389\/fmicb.2016.01981","article-title":"Outer membrane vesicles and soluble factors released by probiotic Escherichia coli Nissle 1917 and commensal ECOR63 enhance barrier function by regulating expression of tight junction proteins in intestinal epithelial cells","volume":"7","author":"Alvarez","year":"2016","journal-title":"Front Microbiol"},{"key":"2026051508375280000_bib3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/AEM.02711-19","article-title":"The ZKIR assay, a real-time PCR method for the detection of Klebsiella pneumoniae and closely related species in environmental samples","volume":"86","author":"Barbier","year":"2020","journal-title":"Appl Environ Microb"},{"key":"2026051508375280000_bib4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.7554\/eLife.65088","article-title":"Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3","volume":"10","author":"Beghini","year":"2021","journal-title":"eLife"},{"key":"2026051508375280000_bib5","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1159\/000342079","article-title":"Probiotic mechanisms of action","volume":"61","author":"Bermudez-Brito","year":"2012","journal-title":"Ann Nutr Metab"},{"key":"2026051508375280000_bib7","doi-asserted-by":"publisher","first-page":"D445","DOI":"10.1093\/nar\/gkz862","article-title":"The MetaCyc data base of metabolic pathways and enzymes: a 2019 update","volume":"48","author":"Caspi","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"2026051508375280000_bib8","doi-asserted-by":"publisher","first-page":"7767","DOI":"10.1128\/AEM.01477-07","article-title":"Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly","volume":"73","author":"Collado","year":"2007","journal-title":"Appl Environ Microb"},{"key":"2026051508375280000_bib9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/microbiolspec.MBP-0006-2014","article-title":"Commensal and pathogenic Escherichia coli metabolism in the gut","volume":"3","author":"Conway","year":"2015","journal-title":"Microbiol Spectr"},{"key":"2026051508375280000_bib10","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.tmaid.2018.11.013","article-title":"Do probiotics prevent colonization with multi-resistant Enterobacteriaceae during travel? A randomized controlled trial","volume":"27","author":"Dall","year":"2019","journal-title":"Travel Med Infect Dis"},{"key":"2026051508375280000_bib11","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.jhin.2017.02.001","article-title":"Is faecal microbiota transplantation an option to eradicate highly drug-resistant enteric bacteria carriage?","volume":"95","author":"Davido","year":"2017","journal-title":"J Hosp Infect"},{"key":"2026051508375280000_bib12","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.jhin.2018.02.018","article-title":"Clearance of carbapenem-resistant Enterobacteriaceae vs vancomycin-resistant enterococci carriage after faecal microbiota transplant: a prospective comparative study","volume":"99","author":"Dinh","year":"2018","journal-title":"J Hosp Infect"},{"key":"2026051508375280000_bib13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/microorganisms8020166","article-title":"Bacterial, gut microbiome-modifying therapies to defend against multidrug resistant organisms","volume":"8","author":"Feehan","year":"2020","journal-title":"Microorganisms"},{"key":"2026051508375280000_bib14","doi-asserted-by":"publisher","first-page":"878","DOI":"10.1038\/s41586-024-07960-6","article-title":"Commensal consortia decolonize Enterobacteriaceae via ecological control","volume":"633","author":"Furuichi","year":"2024","journal-title":"Nature"},{"key":"2026051508375280000_bib15","doi-asserted-by":"publisher","first-page":"25141","DOI":"10.3390\/ijms161025141","article-title":"Chorianopoulos N. Detection and identification of probiotic Lactobacillus plantarum strains by multiplex PCR using RAPD-derived primers","volume":"16","author":"Galanis","year":"2015","journal-title":"Int J Mol Sci"},{"key":"2026051508375280000_bib47_177_173826","doi-asserted-by":"publisher","first-page":"4075","DOI":"10.1128\/IAI.01449-05","article-title":"Escherichia coli Nissle 1917 ameliorates experimental colitis via toll-like receptor 2- and toll-like receptor 4-dependent pathways","volume":"74","author":"Grabig","year":"2006","journal-title":"Infect Immun"},{"key":"2026051508375280000_bib17","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1007\/s00431-007-0419-x","article-title":"The probiotic Escherichia coli strain Nissle 1917 (EcN) stops acute diarrhoea in infants and toddlers","volume":"166","author":"Henker","year":"2007","journal-title":"Eur J Pediatr"},{"key":"2026051508375280000_bib18","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1016\/j.cmi.2018.12.009","article-title":"A 5-day course of oral antibiotics followed by faecal transplantation to eradicate carriage of multidrug-resistant Enterobacteriaceae: a randomized clinical trial","volume":"25","author":"Huttner","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"2026051508375280000_bib19","doi-asserted-by":"publisher","first-page":"2270","DOI":"10.3390\/antibiotics13100924","article-title":"In vitro and in vivo activity of new strains of Bacillus subtilis against ESBL-producing Escherichia coli: an experimental study","volume":"132","author":"Ishnaiwer","year":"2022","journal-title":"J Appl Microbiol"},{"key":"2026051508375280000_bib20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/19490976.2024.2347021","article-title":"Efficacy of an inulin-based treatment on intestinal colonization by multidrug-resistant E. coli: insight into the mechanism of action","volume":"16","author":"Ishnaiwer","year":"2024","journal-title":"Gut Microbes"},{"key":"2026051508375280000_bib21","doi-asserted-by":"publisher","first-page":"e00136","DOI":"10.1128\/CMR.00136-21","article-title":"Intestinal bacteriophage therapy: looking for optimal efficacy","volume":"34","author":"Javaudin","year":"2021","journal-title":"Clin Microbiol Rev"},{"key":"2026051508375280000_bib22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fimmu.2019.01203","article-title":"Commensal bacteria: an emerging player in defense against respiratory pathogens","volume":"10","author":"Khan","year":"2019","journal-title":"Front Immunol"},{"key":"2026051508375280000_bib23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/19490976.2021.2025017","article-title":"The evolution and competitive strategies of Akkermansia muciniphila in the gut","volume":"14","author":"Kim","year":"2022","journal-title":"Gut Microbes"},{"key":"2026051508375280000_bib24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2021.609459","article-title":"Futuristic non-antibiotic therapies to combat antibiotic resistance: a review","volume":"12","author":"Kumar","year":"2021","journal-title":"Front Microbiol"},{"key":"2026051508375280000_bib25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/microorganisms10081558","article-title":"Bacteriocin-producing Escherichia coli isolated from the gastrointestinal tract of farm animals: prevalence, molecular characterization and potential for application","volume":"10","author":"Kuznetsova","year":"2022","journal-title":"Microorganisms"},{"key":"2026051508375280000_bib26","doi-asserted-by":"publisher","first-page":"2876","DOI":"10.1128\/IAI.00059-09","article-title":"Precolonized human commensal Escherichia coli strains serve as a barrier to E. coli O157:H7 growth in the streptomycin-treated mouse intestine","volume":"77","author":"Leatham","year":"2009","journal-title":"Infect Immun"},{"key":"2026051508375280000_bib45_507_173126","doi-asserted-by":"publisher","first-page":"9733","DOI":"10.1038\/s41467-025-64714-2","article-title":"Multi-layered ecological interactions determine growth of clinical antibiotic-resistant strains within human microbiomes","volume":"16","author":"Le\u00f3n-Sampedro","year":"2025","journal-title":"Nat Commun"},{"key":"2026051508375280000_bib28","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-020-17041-7","article-title":"Analysis of compositions of microbiomes with bias correction","volume":"11","author":"Lin","year":"2020","journal-title":"Nat Commun"},{"key":"2026051508375280000_bib30","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.cmi.2019.08.019","article-title":"Probiotics for intestinal decolonization of ESBL-producing Enterobacteriaceae: a randomized, placebo-controlled clinical trial","volume":"26","author":"Ljungquist","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"2026051508375280000_bib48_433_173926","doi-asserted-by":"publisher","first-page":"1501174","DOI":"10.3389\/fimmu.2025.1501174","article-title":"Outer membrane vesicles derived from probiotic Escherichia coli Nissle 1917 promote metabolic remodeling and M1 polarization of RAW264.7 macrophages","volume":"16","author":"Ma","year":"2025","journal-title":"Front Immunol"},{"key":"2026051508375280000_bib31","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.jinf.2017.04.008","article-title":"Impact of faecal microbiota transplantation on mouse digestive colonization with two extensively resistant bacteria","volume":"75","author":"Mahieu","year":"2017","journal-title":"J Infect"},{"key":"2026051508375280000_bib32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0053957","article-title":"Nutritional basis for colonization resistance by human commensal Escherichia coli strains HS and Nissle 1917 against E. coli O157:H7 in the mouse intestine","volume":"8","author":"Maltby","year":"2013","journal-title":"PLoS One"},{"key":"2026051508375280000_bib44_512_161326","doi-asserted-by":"publisher","first-page":"lxad223","DOI":"10.1093\/jambio\/lxad223","article-title":"Pantoprazole promotes sus-tained intestinal carriage of multidrug-resistant Escherichia coli in amoxicillin-treated mice","volume":"134","author":"Moaligou","year":"2023","journal-title":"J Appl Microbiol"},{"key":"2026051508375280000_bib33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/AAC.01293-16","article-title":"Escherichia coli probiotic strain ED1a in pigs has a limited impact on the gut carriage of extended-spectrum \u03b2-lactamase-producing E. coli","volume":"61","author":"Mourand","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"2026051508375280000_bib34","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1099\/00221287-148-1-257","article-title":"Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set","volume":"148","author":"Nadkarni","year":"2002","journal-title":"Microbiology"},{"key":"2026051508375280000_bib35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fmicb.2016.00172","article-title":"Q-PCR based culture-independent enumeration and detection of Enterobacter: an emerging environmental human pathogen in riverine systems and potable water","volume":"7","author":"Patel","year":"2016","journal-title":"Front Microbiol"},{"key":"2026051508375280000_bib36","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1016\/j.cmi.2019.04.006","article-title":"Faecal microbiota transplantation for eradicating carriage of multidrug-resistant organisms: a systematic review","volume":"25","author":"Saha","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"2026051508375280000_bib38","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jff.2020.104080","article-title":"Probiotics as an alternative antimicrobial therapy: current reality and future directions","volume":"73","author":"Silva","year":"2020","journal-title":"J Funct Foods"},{"key":"2026051508375280000_bib39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13104-018-3293-x","article-title":"Fecal microbiota transplantation against intestinal colonization by extended-spectrum beta-lactamase producing Enterobacteriaceae: a proof of principle study","volume":"11","author":"Singh","year":"2018","journal-title":"BMC Res Notes"},{"key":"2026051508375280000_bib40","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1084\/jem.20181639","article-title":"Inhibiting antibiotic-resistant Enterobacteriaceae by microbiota-mediated intracellular acidification","volume":"216","author":"Sorbara","year":"2019","journal-title":"J Exp Med"},{"key":"2026051508375280000_bib41","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1099\/jmm.0.025874-0","article-title":"Testing probiotic strain Escherichia coli Nissle 1917 (Mutaflor) for its ability to reduce carriage of multidrug-resistant E. coli by elderly residents in long-term care facilities","volume":"60","author":"Tannock","year":"2011","journal-title":"J Med Microbiol"},{"key":"2026051508375280000_bib42","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1111\/lam.12359","article-title":"Development of a real-time PCR for Escherichia coli based on gadE, an acid response regulatory gene","volume":"60","author":"Tillman","year":"2015","journal-title":"Lett Appl Microbiol"},{"key":"2026051508375280000_bib43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-025-61327-7","article-title":"Suppression of gut colonization by multidrug-resistant Escherichia coli clinical isolates through co-operative niche exclusion","volume":"16","author":"Wende","year":"2025","journal-title":"Nat Commun"},{"key":"2026051508375280000_bib44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fpubh.2020.578089","article-title":"Do probiotics during in-hospital antibiotic treatment prevent colonization of gut microbiota with multi-drug-resistant bacteria? A randomized placebo-controlled trial comparing Saccharomyces to a mixture of Lactobacillus, Bifidobacterium, and Saccharomyces","volume":"8","author":"Wie\u00ebrs","year":"2020","journal-title":"Front Public Health"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxag041\/66799530\/lxag041.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/3\/lxag041\/66799530\/lxag041.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/3\/lxag041\/66799530\/lxag041.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:37:58Z","timestamp":1778848678000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxag041\/8466419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,6]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,5]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxag041","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,3]]},"published":{"date-parts":[[2026,2,6]]},"article-number":"lxag041"}}