{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T01:13:51Z","timestamp":1792977231342,"version":"4.5.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONACYT","doi-asserted-by":"publisher","award":["315817"],"award-info":[{"award-number":["315817"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"CONACYT","doi-asserted-by":"publisher","award":["IN216517"],"award-info":[{"award-number":["IN216517"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Type 2 diabetes mellitus (T2DM) is a risk factor for periodontitis; however, its relationship with the oral microbiome remains poorly defined. Here, we characterize taxonomic composition and predicted microbial functions in the salivary microbiome of T2DM and non-diabetic patients with periodontitis, while evaluating the influence of disease stage, smoking, and sex.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>We analyzed saliva from 100 periodontitis patients (50 T2DM, 50 non-diabetic controls) using 16S rRNA V3\u2013V4 sequencing, selecting a screened, deliberately balanced cohort to ensure robust comparison. \u03b1-diversity did not differ significantly across diabetes status, disease stage, smoking, or sex. However, \u03b2-diversity analysis revealed a statistically significant differences in microbial community composition between T2DM and non-diabetic controls (R2\u00a0=\u00a00.016, p\u00a0=\u00a00.029). Differential abundance analysis showed enrichment of Neisseria (p\u00a0=\u00a00.02, Wilcoxon test with Bonferroni correction) and Corynebacterium matruchotii (p\u00a0=\u00a00.002) in T2DM patients, whereas Haemophilus sputorum was enriched in mild periodontitis. Among smokers, Neisseria elongata (p\u00a0=\u00a00.009) was more abundant than in non-smokers. Female patients exhibited higher abundances of Haemophilus, Neisseria, Gemella, Aggregatibacter, Corynebacterium, and Cardiobacterium (p\u00a0&amp;lt;\u00a00.02), whereas Megasphaera was enriched in males (p\u00a0=\u00a00.04). PICRUSt-based predictions indicated TDM2-associated enrichment of pathway related to virulence, nutrient acquisition, and pyridoxal-5\u2032-phosphate biosynthesis, particularly in moderate and severe periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>T2DM is associated with distinct shifts in salivary microbial composition and predicted function, revealing taxa and pathways linked to periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag051","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:44:01Z","timestamp":1772109841000},"source":"Crossref","is-referenced-by-count":1,"title":["Integrative analysis of bacterial diversity in patients with type 2 diabetes mellitus and periodontitis using a case-control microbiome approach"],"prefix":"10.1093","volume":"137","author":[{"given":"H\u00e9ctor","family":"Mart\u00ednez-Gregorio","sequence":"first","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Vallejo-Lecuona","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Ambriz-Barrera","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Ruiz-De-La-Cruz","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julieta Aurora","family":"Morales-Valdez","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto Arturo","family":"Rojas-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Dana-Farber Cancer Institute Department of Medical Oncology, , Boston, MA 02215 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Sof\u00eda","family":"Jim\u00e9nez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Monroy-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria Luz","family":"Paniagua-Contreras","sequence":"additional","affiliation":[{"name":"Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Estela","family":"D\u00edaz-Vel\u00e1squez","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2200-9706","authenticated-orcid":false,"given":"Felipe","family":"Vaca-Paniagua","sequence":"additional","affiliation":[{"name":"National Health Laboratory, Molecular Diagnosis and Environmental Effects in Chronic-Degenerative Diseases, Faculty of Higher Education Iztacala, National Autonomous University of Mexico , Tlalnepantla 54090 ,","place":["Mexico"]},{"name":"Unit of Biomedicine, Faculty of Higher Education Iztacala, National Autonomous University of Mexico (UNAM) , Tlalnepantla 54090 ,","place":["Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"2026051508381336400_bib1","doi-asserted-by":"publisher","first-page":"20584","DOI":"10.1038\/s41598-023-47905-z","article-title":"Characterization of gut microbiome composition in Iranian patients with nonalcoholic fatty liver disease and nonalcoholic steatohepatitis","volume":"13","author":"Abdollahiyan","year":"2023","journal-title":"Sci Rep"},{"key":"2026051508381336400_bib2","doi-asserted-by":"publisher","first-page":"2861","DOI":"10.1038\/s41598-022-06907-z","article-title":"Supragingival microbiome alternations as a consequence of smoking different tobacco types and its relation to dental caries","volume":"12","author":"Al-Marzooq","year":"2022","journal-title":"Sci Rep"},{"key":"2026051508381336400_bib3","doi-asserted-by":"publisher","first-page":"S54","DOI":"10.4103\/jpbs.jpbs_515_22","article-title":"Periodontal Diseases and diabetes Mellitus: a systematic review","volume":"15","author":"Alwithanani","year":"2023","journal-title":"Journal of Pharmacy and Bioallied Sciences"},{"key":"2026051508381336400_bib4","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1111\/jre.12968","article-title":"Herpes Simplex Virus type 1 in subgingival plaque and periodontal diseases. Meta-analysis of observational studies","volume":"57","author":"Arduino","year":"2022","journal-title":"J Periodontal Res"},{"key":"2026051508381336400_bib5","doi-asserted-by":"publisher","first-page":"2957","DOI":"10.2337\/dc22-0616","article-title":"Diabetes-related excess mortality in Mexico: a comparative analysis of national death registries between 2017\u20132019 and 2020","volume":"45","author":"Bello-Chavolla","year":"2022","journal-title":"Diabetes Care"},{"key":"2026051508381336400_bib6","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2026051508381336400_bib7","doi-asserted-by":"publisher","first-page":"e1289","DOI":"10.1210\/clinem\/dgad287","article-title":"Type 2 diabetes mellitus and periodontitis: bidirectional association in population-based 15-year retrospective cohorts","volume":"108","author":"Chien","year":"2023","journal-title":"J Clin Endocrinol Metab"},{"key":"2026051508381336400_bib8","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.3390\/jcm12031147","article-title":"Association between type 1 diabetes mellitus and periodontal diseases","volume":"12","author":"Costa","year":"2023","journal-title":"J Clin Med"},{"key":"2026051508381336400_bib9","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","article-title":"PICRUSt2 for prediction of metagenome functions","volume":"38","author":"Douglas","year":"2020","journal-title":"Nat Biotechnol"},{"key":"2026051508381336400_bib10","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1902\/jop.2008.070455","article-title":"Microbiologic and immunologic characteristics of periodontal disease in Hispanic Americans with type 2 diabetes","volume":"79","author":"Ebersole","year":"2008","journal-title":"J Periodontol"},{"key":"2026051508381336400_bib11","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.archoralbio.2019.05.025","article-title":"Whole metagenomic shotgun sequencing of the subgingival microbiome of diabetics and non-diabetics with different periodontal conditions","volume":"104","author":"Farina","year":"2019","journal-title":"Arch Oral Biol"},{"key":"2026051508381336400_bib12","doi-asserted-by":"publisher","first-page":"2075","DOI":"10.1038\/ismej.2017.73","article-title":"A tale of two risks: smoking, diabetes and the subgingival microbiome","volume":"11","author":"Ganesan","year":"2017","journal-title":"ISME J"},{"key":"2026051508381336400_bib13","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1111\/j.1600-0757.2012.00457.x","article-title":"Risk factors for periodontal disease","volume":"62","author":"Genco","year":"2013","journal-title":"Periodontol 2000"},{"key":"2026051508381336400_bib14","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1038\/ismej.2011.191","article-title":"Distinct and complex bacterial profiles in Human periodontitis and health revealed by 16S pyrosequencing","volume":"6","author":"Griffen","year":"2012","journal-title":"ISME J"},{"key":"2026051508381336400_bib15","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.1186\/s12903-024-05135-3","article-title":"Salivary microbiome variations in type 2 diabetes mellitus patients with different stages of periodontitis","volume":"24","author":"Gu","year":"2024","journal-title":"BMC Oral Health"},{"key":"2026051508381336400_bib16","doi-asserted-by":"publisher","first-page":"2847","DOI":"10.1093\/bioinformatics\/btw313","article-title":"Complex heatmaps reveal patterns and correlations in multidimensional genomic data","volume":"32","author":"Gu","year":"2016","journal-title":"Bioinformatics"},{"key":"2026051508381336400_bib17","doi-asserted-by":"publisher","first-page":"624","DOI":"10.3390\/ijerph22040624","article-title":"The Global burden of periodontal disease: a narrative review on unveiling socioeconomic and health challenges","volume":"22","author":"Hashim","year":"2025","journal-title":"Int J Environ Res Public Health"},{"key":"2026051508381336400_bib18","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1111\/odi.14516","article-title":"WHO\u2019s Global Oral Health Status Report 2022: actions, discussion and implementation","volume":"30","author":"Jain","year":"2024","journal-title":"Oral Dis"},{"key":"2026051508381336400_bib19","doi-asserted-by":"publisher","first-page":"e0172647","DOI":"10.1371\/journal.pone.0172647","article-title":"Salivary inflammatory markers and microbiome in normoglycemic lean and obese children compared to obese children with type 2 diabetes","volume":"12","author":"Janem","year":"2017","journal-title":"PLoS One"},{"key":"2026051508381336400_bib20","doi-asserted-by":"publisher","first-page":"e0174782","DOI":"10.1371\/journal.pone.0174782","article-title":"Relative abundance of total subgingival plaque-specific bacteria in salivary microbiota reflects the overall periodontal condition in patients with periodontitis","volume":"12","author":"Kageyama","year":"2017","journal-title":"PLoS One"},{"key":"2026051508381336400_bib21","doi-asserted-by":"publisher","first-page":"17038","DOI":"10.1038\/nrdp.2017.38","article-title":"Periodontal diseases","volume":"3","author":"Kinane","year":"2017","journal-title":"Nat Rev Dis Primers"},{"key":"2026051508381336400_bib22","doi-asserted-by":"publisher","first-page":"1469414","DOI":"10.3389\/fmicb.2024.1469414","article-title":"Periodontitis: etiology, conventional treatments, and emerging bacteriophage and predatory bacteria therapies","volume":"15","author":"\u0141asica","year":"2024","journal-title":"Front Microbiol"},{"key":"2026051508381336400_bib23","doi-asserted-by":"publisher","first-page":"358","DOI":"10.5051\/jpis.2002420121","article-title":"Efficacy of salivary versus subgingival bacterial sampling for the detection and quantification of periodontal pathogens","volume":"50","author":"Lee","year":"2020","journal-title":"Journal of Periodontal & Implant Science"},{"key":"2026051508381336400_bib24","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1111\/idj.12317","article-title":"The Association between Oral Hygiene and Periodontitis: a systematic review and meta-analysis","volume":"67","author":"Lertpimonchai","year":"2017","journal-title":"Int Dent J"},{"key":"2026051508381336400_bib50_222_121326","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41596-025-01239-4","author":"Liu","year":"2025"},{"key":"2026051508381336400_bib25","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.105839","article-title":"Sex differences in the oral microbiome, host traits, and their causal relationships","volume-title":"iScience","author":"Liu","year":"2023"},{"key":"2026051508381336400_bib26","doi-asserted-by":"publisher","first-page":"E791","DOI":"10.1073\/pnas.1522149113","article-title":"Biogeography of a Human oral microbiome at the micron scale","volume":"113","author":"Mark\u00a0Welch","year":"2016","journal-title":"Proc Natl Acad Sci"},{"key":"2026051508381336400_bib27","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-96881-6_5","article-title":"Parasites in periodontal health and disease: a systematic review and meta-analysis","volume-title":"Periodontitis: Advances in Experimental Research","author":"Martin-Garcia","year":"2022"},{"key":"2026051508381336400_bib28","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1177\/0022034520913818","article-title":"Oral microbiome signatures in diabetes mellitus and periodontal disease","volume":"99","author":"Matsha","year":"2020","journal-title":"J Dent Res"},{"key":"2026051508381336400_bib29","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1093\/cvr\/cvz201","article-title":"Periodontitis is associated with hypertension: a systematic review and meta-analysis","volume":"116","author":"Mu\u00f1oz\u00a0Aguilera","year":"2020","journal-title":"Cardiovasc Res"},{"key":"2026051508381336400_bib30","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/S0140-6736(23)01301-6","article-title":"Global, regional, and national burden of diabetes from 1990 to 2021, with projections of prevalence to 2050: a systematic analysis for the Global burden of Disease Study 2021","volume":"402","author":"Ong","year":"2023","journal-title":"The Lancet"},{"key":"2026051508381336400_bib31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcimb.2022.965159","article-title":"Oral microbiota and periodontitis severity among Hispanic adults","volume":"12","author":"Ortiz","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"2026051508381336400_bib32","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1186\/s12866-023-02779-z","article-title":"The mediating roles of the oral microbiome in saliva and subgingival sites between E-cigarette smoking and gingival inflammation","volume":"23","author":"Park","year":"2023","journal-title":"BMC Microbiol"},{"key":"2026051508381336400_bib33","doi-asserted-by":"publisher","first-page":"3184","DOI":"10.3390\/ijerph16173184","article-title":"Subgingival microbiota of Mexicans with type 2 diabetes with different periodontal and metabolic conditions","volume":"16","author":"Rodr\u00edguez-Hern\u00e1ndez","year":"2019","journal-title":"Int J Environ Res Public Health"},{"key":"2026051508381336400_bib34","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1002\/JPER.18-0167","article-title":"The salivary microbiome of Diabetic and Non-Diabetic Adults with periodontal disease","volume":"90","author":"Sabharwal","year":"2019","journal-title":"J Periodontol"},{"key":"2026051508381336400_bib35","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.micpath.2019.01.009","article-title":"Relative reduction of biological and phylogenetic diversity of the oral microbiota of diabetes and pre-diabetes patients","volume":"128","author":"Saeb","year":"2019","journal-title":"Microb Pathog"},{"key":"2026051508381336400_bib36","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/dnares\/dst037","article-title":"Dysbiosis of salivary microbiota in inflammatory bowel disease and its association with oral immunological biomarkers","volume":"21","author":"Said","year":"2014","journal-title":"DNA Res"},{"key":"2026051508381336400_bib37","doi-asserted-by":"publisher","first-page":"27","DOI":"10.2337\/diacare.28.1.27","article-title":"Periodontal disease and mortality in type 2 diabetes","volume":"28","author":"Saremi","year":"2005","journal-title":"Diabetes Care"},{"key":"2026051508381336400_bib38","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/s41396-019-0544-3","article-title":"The subgingival microbiome associated with periodontitis in type 2 diabetes mellitus","volume":"14","author":"Shi","year":"2020","journal-title":"ISME J"},{"key":"2026051508381336400_bib39","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/s41396-019-0544-3","article-title":"The subgingival microbiome associated with periodontitis in type 2 diabetes mellitus","volume":"14","author":"Shi","year":"2020","journal-title":"ISME J"},{"key":"2026051508381336400_bib40","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1902\/jop.2010.100044","article-title":"Sex differences in destructive periodontal disease: a systematic review","volume":"81","author":"Shiau","year":"2010","journal-title":"J Periodontol"},{"key":"2026051508381336400_bib41","doi-asserted-by":"publisher","first-page":"15363","DOI":"10.1038\/s41598-020-72035-1","article-title":"Alteration of salivary microbiome in periodontitis with or without type-2 diabetes mellitus and metformin treatment","volume":"10","author":"Sun","year":"2020","journal-title":"Sci Rep"},{"key":"2026051508381336400_bib42","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.heliyon.2023.e22110","article-title":"Oral microbiota in periodontitis patients with and without type 2 diabetes mellitus and their shifts after the nonsurgical periodontal therapy","volume":"9","author":"Wang","year":"2023","journal-title":"Heliyon"},{"key":"2026051508381336400_bib43","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1186\/s12903-020-01180-w","article-title":"Epidemiologic relationship between periodontitis and type 2 diabetes mellitus","volume":"20","author":"Wu","year":"2020","journal-title":"BMC Oral Health"},{"key":"2026051508381336400_bib44","doi-asserted-by":"publisher","first-page":"2435","DOI":"10.1038\/ismej.2016.37","article-title":"Cigarette smoking and the oral microbiome in a large study of American adults","volume":"10","author":"Wu","year":"2016","journal-title":"ISME J"},{"key":"2026051508381336400_bib45","doi-asserted-by":"publisher","first-page":"btad470","DOI":"10.1093\/bioinformatics\/btad470","article-title":"Ggpicrust2: an R package for PICRUSt2 predicted functional profile analysis and visualization","volume":"39","author":"Yang","year":"2023","journal-title":"Bioinformatics"},{"key":"2026051508381336400_bib46","doi-asserted-by":"publisher","first-page":"D643","DOI":"10.1093\/nar\/gkt1209","article-title":"The SILVA and \u2018All-Species Living Tree Project (LTP)\u2019 Taxonomic frameworks","volume":"42","author":"Yilmaz","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"2026051508381336400_bib47","doi-asserted-by":"publisher","first-page":"614","DOI":"10.1093\/bioinformatics\/btt593","article-title":"PEAR: a fast and accurate Illumina paired-end reAd mergeR","volume":"30","author":"Zhang","year":"2014","journal-title":"Bioinformatics"},{"key":"2026051508381336400_bib48","doi-asserted-by":"publisher","first-page":"e61516","DOI":"10.1371\/journal.pone.0061516","article-title":"Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing","volume":"8","author":"Zhou","year":"2013","journal-title":"PLoS One"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxag051\/67136367\/lxag051.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/3\/lxag051\/67136367\/lxag051.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/3\/lxag051\/67136367\/lxag051.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:38:16Z","timestamp":1778848696000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxag051\/8499643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,26]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,5]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxag051","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,3]]},"published":{"date-parts":[[2026,2,26]]},"article-number":"lxag051"}}