{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:11Z","timestamp":1787009051531,"version":"build-2736575974"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2020MC184"],"award-info":[{"award-number":["ZR2020MC184"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shandong Medical and Health Science and Technology Development Plan Project","award":["202203060106"],"award-info":[{"award-number":["202203060106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The increasing burden of multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) infections highlights the need for antibiotic-sparing approaches. This study isolated and characterized a lytic bacteriophage active against MDR P. aeruginosa and evaluated its potential for infection control and milk decontamination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>The lytic bacteriophage vB_PaeM_QR7 was isolated from sewage and characterized using morphological, phenotypic, and genomic analyses. Whole-genome sequencing revealed a 45\u2009421-bp linear double-stranded DNA genome with no predicted virulence determinants and antibiotic resistance genes. vB_PaeM_QR7 showed high lytic efficiency (optimal MOI\u00a0=\u00a01\u00a0\u00d7\u00a010\u22125), stability across 30\u201350\u00b0C and pH 4\u201311, a short latent period, and a burst size of 92.43\u00a0\u00b1\u00a052.29 PFU cell\u22121. The bacteriophage inhibited biofilm formation by 65.88\u00a0\u00b1\u00a00.04% and reduced established biofilms by 71.27\u00a0\u00b1\u00a00.05%. In milk, vB_PaeM_QR7 reduced bacterial loads by 5.40\u00a0\u00b1\u00a00.12 log10 CFU mL\u22121 , and in the Galleria mellonella infection model, it increased larval survival by 50%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>vB_PaeM_QR7 is a newly identified lytic bacteriophage with activity against 10 MDR P. aeruginosa isolates, including cephalosporin- and carbapenem-resistant strains, supporting its further development as an alternative antimicrobial agent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag057","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T12:41:57Z","timestamp":1771936917000},"source":"Crossref","is-referenced-by-count":1,"title":["Characterization and therapeutic potential of vB_PaeM_QR7: a highly efficient lytic bacteriophage against multidrug-resistant\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1093","volume":"137","author":[{"given":"Jiaqi","family":"Pu","sequence":"first","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , Zibo 255000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengge","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , Zibo 255000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , Zibo 255000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deshu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Medicine, Shandong University of Technology , Zibo 255000 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