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This study aimed to isolate phages against bovine-origin MDR K. pneumoniae and evaluate their therapeutic potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>We successfully isolated five lytic bacteriophages (phages) (vB_Kpn_Y1\u2013Y5) from hospital sewage samples. They showed favorable temperature and pH stability, efficient lytic cycles, and narrow host ranges. Whole-genome analysis further classified these phages into three categories belonging to the families Siphoviridae and Podoviridae within the class of Caudoviricetes. This genetic diversity provides a foundation for constructing a phage cocktail. A phage cocktail constructed from these phages exhibited synergistic antibacterial effects in vitro, with superior inhibition and duration compared to single phages. In a mouse bacteremia model, the phage cocktail therapy significantly increased the survival rate of mice from 50% to 91.7% (P &amp;lt; 0.05), effectively reduced bacterial loads in multiple organs (P\u00a0&amp;lt;\u00a00.0001), alleviated tissue inflammatory damage, and showed no obvious toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The genetic diversity of the phages underpinned their synergy. The phage cocktail demonstrated better efficacy than monotherapy both in vitro and in vivo.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag113","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:21:26Z","timestamp":1778498486000},"source":"Crossref","is-referenced-by-count":0,"title":["Novel phage exhibits effective\n                    <i>in vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    activity against bovine-derived multidrug-resistant\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1093","volume":"137","author":[{"given":"Keyu","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Life Sciences, Shihezi University , Shihezi City, Xinjiang 832003 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexuan","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shihezi 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