{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:05:07Z","timestamp":1785423907571,"version":"3.56.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172284"],"award-info":[{"award-number":["32172284"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010242","name":"Jiangsu Planned Projects for Postdoctoral Research Funds","doi-asserted-by":"publisher","award":["2021K316C"],"award-info":[{"award-number":["2021K316C"]}],"id":[{"id":"10.13039\/501100010242","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lianyungang Postdoctoral Research Funding Program","award":["LYG20210001"],"award-info":[{"award-number":["LYG20210001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Vibrio parahaemolyticus is a major zoonotic pathogen. Its outer membrane proteins (OMPs) are key targets for developing vaccines. This study aimed to evaluate the potential of OmpU and VP0802 as subunit vaccine candidates against Vibrio species.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Unlike prior studies relying on qualitative survival endpoints and narrow strain panels, we employed serum bactericidal assays and an expanded strain panel to quantify cross-protective efficacy. The recombinant proteins OmpU and VP0802 were cloned, expressed, and purified. Their native abundance, surface exposure, immunogenicity, and protective efficacy were evaluated. Both recombinant OMPs exhibited strong binding to whole-cell murine antisera in an indirect enzyme-linked immunosorbent assay (titer 1:81\u2009000). Confocal microscopy further confirmed high surface availability, with specific anti-OmpU and anti-VP0802 sera binding to bacterial cells. Immunization results showed that OmpU induced high-titer antisera (up to 1:243\u2009000), accompanied by broad cross-reactivity and bactericidal activity against different V. parahaemolyticus serotypes and multiple Vibrio species, with a potent bactericidal antibody titer of 1:512. In contrast, VP0802 generated lower antibody titers (up to 1:9000), reduced immunoblot band, weaker immunofluorescence signals, and significantly reduced bactericidal activity (1:32).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>OmpU represents a promising cross-protective subunit vaccine candidate against multiple Vibrio species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag129","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:32:31Z","timestamp":1780399951000},"source":"Crossref","is-referenced-by-count":0,"title":["OmpU elicits cross-protective bactericidal activity against\n                    <i>Vibrio<\/i>\n                    species: a surface-exposed antigen with vaccine potential"],"prefix":"10.1093","volume":"137","author":[{"given":"Shi","family":"Yuan","sequence":"first","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Feng","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Feng","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saikun","family":"Pan","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3682-1858","authenticated-orcid":false,"given":"Wenbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Bioresources and Environment , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Co-Innovation Center of Jiangsu Marine Bio-industry Technology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Ocean University, Jiangsu Key Laboratory of Marine Biotechnology , Lianyungang, 222005 Jiangsu ,","place":["China"]},{"name":"Jiangsu Institute of Marine Resources Development , Lianyungang, 222005 Jiangsu ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"2026073010425269300_bib1","doi-asserted-by":"publisher","first-page":"106791","DOI":"10.1016\/j.pep.2025.106791","article-title":"Solubilization and refolding of inclusion bodies by detergents","volume":"236","author":"Arakawa","year":"2025","journal-title":"Protein Expr Purif"},{"key":"2026073010425269300_bib2","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1159\/000443646","article-title":"Naturally occurring IgG antibodies provide innate protection against Vibrio cholerae bacteremia by recognition of the outer membrane protein U","volume":"8","author":"Aung","year":"2016","journal-title":"J Innate Immun"},{"key":"2026073010425269300_bib3","doi-asserted-by":"publisher","first-page":"63","DOI":"10.3354\/dao02206","article-title":"Immune response in Lutjanus erythropterus induced by the major outer membrane protein (OmpU) of Vibrio alginolyticus","volume":"90","author":"Cai","year":"2010","journal-title":"Dis Aquat Organ"},{"key":"2026073010425269300_bib4","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/j.fsi.2012.11.015","article-title":"Identification and immunogenicity of immunodominant mimotopes of outer membrane protein U (OmpU) of Vibrio mimicus from phage display peptide library","volume":"34","author":"Cen","year":"2013","journal-title":"Fish Shellfish Immunol"},{"key":"2026073010425269300_bib5","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1128\/jb.178.2.524-530.1996","article-title":"Porins of Vibrio cholerae: purification and characterization of OmpU","volume":"178","author":"Chakrabarti","year":"1996","journal-title":"J Bacteriol"},{"key":"2026073010425269300_bib6","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1038\/s41467-020-14655-9","article-title":"Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface","volume":"11","author":"Dom\u00ednguez-Medina","year":"2020","journal-title":"Nat Commun"},{"key":"2026073010425269300_bib7","doi-asserted-by":"publisher","first-page":"105267","DOI":"10.1016\/j.micpath.2021.105267","article-title":"Structure, regulation, and host interaction of outer membrane protein U (OmpU) of Vibrio species","volume":"162","author":"Ganie","year":"2022","journal-title":"Microb Pathog"},{"key":"2026073010425269300_bib8","doi-asserted-by":"publisher","first-page":"104419","DOI":"10.1016\/j.micpath.2020.104419","article-title":"Evaluation of the \u03b2-barrel outer membrane protein VP1243 as a candidate antigen for a cross-protective vaccine against Vibrio infections","volume":"147","author":"Gao","year":"2020","journal-title":"Microb Pathog"},{"key":"2026073010425269300_bib9","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/s0167-7799(01)01600-6","article-title":"Antibacterial vaccine design using genomics and proteomics","volume":"19","author":"Grandi","year":"2001","journal-title":"Trends Biotechnol"},{"key":"2026073010425269300_bib10","first-page":"30","article-title":"Alternative method to remove antibacterial antibodies from antisera used for screening of expression libraries","volume":"19","author":"Gruber","year":"1995","journal-title":"BioTechniques"},{"key":"2026073010425269300_bib11","doi-asserted-by":"publisher","first-page":"128646","DOI":"10.1016\/j.ijbiomac.2023.128646","article-title":"Biological importance of arginine: a comprehensive review of the roles in structure, disorder, and functionality of peptides and proteins","volume":"257","author":"Gupta","year":"2024","journal-title":"Int J Biol Macromol"},{"key":"2026073010425269300_bib12","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1016\/j.fsi.2019.12.060","article-title":"Immunization of a novel outer membrane protein from Aeromonas hydrophila simultaneously resisting A. hydrophila and Edwardsiella anguillarum infection in European eels (Angullia angullia)","volume":"97","author":"He","year":"2020","journal-title":"Fish Shellfish Immunol"},{"key":"2026073010425269300_bib13","doi-asserted-by":"publisher","first-page":"10340","DOI":"10.1074\/jbc.REV120.011473","article-title":"Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria","volume":"295","author":"Horne","year":"2020","journal-title":"J Biol Chem"},{"key":"2026073010425269300_bib14","doi-asserted-by":"publisher","first-page":"100602","DOI":"10.1016\/j.jgeb.2025.100602","article-title":"Cloning and expression of outer membrane proteins from Vibrio alginolyticus, and evaluation of their immune response in Epinephelus coioides","volume":"23","author":"Huyen","year":"2025","journal-title":"J Genet Eng Biotechnol"},{"key":"2026073010425269300_bib15","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1007\/978-1-4939-8935-5_26","article-title":"An introduction to performing immunofluorescence staining","volume":"1897","author":"Im","year":"2019","journal-title":"Methods Mol Biol"},{"key":"2026073010425269300_bib16","doi-asserted-by":"publisher","first-page":"77","DOI":"10.3109\/10408418209113506","article-title":"Vibrio parahaemolyticus and related halophilic Vibrios","volume":"10","author":"Joseph","year":"1982","journal-title":"Crit Rev Microbiol"},{"key":"2026073010425269300_bib17","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1111\/jam.14068","article-title":"Immunogenicity study of an expressed outer membrane protein U of Vibrio vulnificus in Japanese eel (Anguilla japonica)","volume":"125","author":"Le","year":"2018","journal-title":"J Appl Microbiol"},{"key":"2026073010425269300_bib18","doi-asserted-by":"publisher","first-page":"705","DOI":"10.3389\/fmicb.2014.00705","article-title":"Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques","volume":"5","author":"Letchumanan","year":"2014","journal-title":"Front Microbiol"},{"key":"2026073010425269300_bib19","doi-asserted-by":"publisher","first-page":"6115","DOI":"10.1016\/j.vaccine.2014.08.077","article-title":"Identification of a novel vaccine candidate by immunogenic screening of Vibrio parahaemolyticus outer membrane proteins","volume":"32","author":"Li","year":"2014","journal-title":"Vaccine"},{"key":"2026073010425269300_bib20","doi-asserted-by":"publisher","first-page":"110440","DOI":"10.1016\/j.fsi.2025.110440","article-title":"Genome-based screening and immunogenicity evaluation of outer membrane protein antigens for Vibrio harveyi subunit vaccine in pearl gentian grouper (Epinephelus lanceolatus \u00d7 Epinephelus fuscoguttatus)","volume":"165","author":"Li","year":"2025","journal-title":"Fish Shellfish Immunol"},{"key":"2026073010425269300_bib21","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.fsi.2015.12.024","article-title":"Construction and immune protection evaluation of recombinant polyvalent OmpAs derived from genetically divergent ompA by DNA shuffling","volume":"49","author":"Li","year":"2016","journal-title":"Fish Shellfish Immunol"},{"key":"2026073010425269300_bib22","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.micres.2019.03.003","article-title":"Molecular mechanisms of Vibrio parahaemolyticus pathogenesis","volume":"222","author":"Li","year":"2019","journal-title":"Microbiol Res"},{"key":"2026073010425269300_bib23","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.imlet.2019.07.006","article-title":"IgE binding activities and in silico epitope prediction of Der f 32 in Dermatophagoides farinae","volume":"213","author":"Li","year":"2019","journal-title":"Immunol Lett"},{"key":"2026073010425269300_bib24","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/s1286-4579(02)01545-9","article-title":"Outer membrane proteins: key players for bacterial adaptation in host niches","volume":"4","author":"Lin","year":"2002","journal-title":"Microbes Infect"},{"key":"2026073010425269300_bib25","doi-asserted-by":"publisher","first-page":"e0119026","DOI":"10.1371\/journal.pone.0119026","article-title":"Outer membrane protein U (OmpU) mediates adhesion of Vibrio mimicus to host cells via two novel N-terminal motifs","volume":"10","author":"Liu","year":"2015","journal-title":"PLoS One"},{"key":"2026073010425269300_bib26","doi-asserted-by":"publisher","first-page":"867","DOI":"10.13343\/j.cnki.wsxb.20150363","article-title":"Immunological cross-reactivity and cross-protection of outer membrane protein OmpU among Vibrio species","volume":"56","author":"Lun","year":"2016","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"2026073010425269300_bib27","doi-asserted-by":"publisher","first-page":"126964","DOI":"10.1016\/j.vaccine.2025.126964","article-title":"Identification of a conserved cryptic epitope with cross-immunoreactivity in outer membrane protein K (OmpK) from Vibrio species","volume":"53","author":"Lun","year":"2025","journal-title":"Vaccine"},{"key":"2026073010425269300_bib28","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1016\/j.fsi.2007.01.004","article-title":"Cloning, expression and immunogenicty analysis of five outer membrane proteins of Vibrio parahaemolyticus zj2003","volume":"23","author":"Mao","year":"2007","journal-title":"Fish Shellfish Immunol"},{"key":"2026073010425269300_bib29","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1111\/j.1745-7270.2007.00339.x","article-title":"Expression and immunogenicity analysis of two iron-regulated outer membrane proteins of Vibrio parahaemolyticus","volume":"39","author":"Mao","year":"2007","journal-title":"Acta Biochim Biophys Sin"},{"key":"2026073010425269300_bib30","doi-asserted-by":"publisher","first-page":"e70365","DOI":"10.1371\/journal.pone.0070365","article-title":"Identification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults","volume":"8","author":"Olaya-Abril","year":"2013","journal-title":"PLoS One"},{"key":"2026073010425269300_bib31","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1007\/978-1-4939-3389-1_34","article-title":"Immunoproteomic approach for screening vaccine candidates from bacterial outer membrane proteins","volume":"1404","author":"Pan","year":"2016","journal-title":"Methods Mol Biol"},{"key":"2026073010425269300_bib32","doi-asserted-by":"publisher","first-page":"5855","DOI":"10.1016\/j.vaccine.2009.07.054","article-title":"Purification and characterization of an immunogenic outer membrane protein of Shigella flexneri 2a","volume":"27","author":"Pore","year":"2009","journal-title":"Vaccine"},{"key":"2026073010425269300_bib33","doi-asserted-by":"publisher","first-page":"805","DOI":"10.3389\/fmicb.2014.00805","article-title":"Roles of thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) in Vibrio parahaemolyticus","volume":"5","author":"Raghunath","year":"2014","journal-title":"Front Microbiol"},{"key":"2026073010425269300_bib34","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.imlet.2012.08.003","article-title":"Immune system and immune responses in fish and their role in comparative immunity study: a model for higher organisms","volume":"148","author":"Rauta","year":"2012","journal-title":"Immunol Lett"},{"key":"2026073010425269300_bib35","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.pep.2005.09.027","article-title":"Single step protocol to purify recombinant proteins with low endotoxin contents","volume":"46","author":"Reichelt","year":"2006","journal-title":"Protein Expr Purif"},{"key":"2026073010425269300_bib36","doi-asserted-by":"publisher","first-page":"20150023","DOI":"10.1098\/rstb.2015.0023","article-title":"Outer membrane protein biogenesis in Gram-negative bacteria","volume":"370:","author":"Rollauer","year":"2015","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"2026073010425269300_bib37","doi-asserted-by":"publisher","first-page":"e03497","DOI":"10.7554\/eLife.03497","article-title":"Evolutionary consequences of intra-patient phage predation on microbial populations","volume":"3","author":"Seed","year":"2014","journal-title":"eLife"},{"key":"2026073010425269300_bib38","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.dci.2005.06.007","article-title":"Antibody repertoire development in teleosts\u2014a review with emphasis on salmonids and Gadus morhua L","volume":"30","author":"Solem","year":"2006","journal-title":"Dev Comp Immunol"},{"key":"2026073010425269300_bib39","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1111\/j.1472-765X.2011.03062.x","article-title":"Identification and evaluation of an outer membrane protein OmpU from a pathogenic Vibrio harveyi isolate as vaccine candidate in turbot (Scophthalmus maximus)","volume":"53","author":"Wang","year":"2011","journal-title":"Lett Appl Microbiol"},{"key":"2026073010425269300_bib40","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1111\/lam.13536","article-title":"Comparative immunogenicity of outer membrane protein K and whole-cell antigens of Vibrio parahaemolyticus for diagnosis","volume":"73","author":"Wang","year":"2021","journal-title":"Lett Appl Microbiol"},{"key":"2026073010425269300_bib41","doi-asserted-by":"publisher","first-page":"625315","DOI":"10.3389\/fmicb.2020.625315","article-title":"Identification of Vibrio parahaemolyticus and Vibrio spp. specific outer membrane proteins by reverse vaccinology and surface proteome","volume":"11","author":"Wang","year":"2020","journal-title":"Front Microbiol"},{"key":"2026073010425269300_bib42","doi-asserted-by":"publisher","first-page":"106111","DOI":"10.1016\/j.pep.2022.106111","article-title":"Recombinant expression and immunogenicity evaluation of a TonB-dependent receptor of Vibrio parahaemolyticus","volume":"197","author":"Wang","year":"2022","journal-title":"Protein Expr Purif"},{"key":"2026073010425269300_bib43","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.1111\/jam.13726","article-title":"Recombinant ApxIV protein enhances protective efficacy against Actinobacillus pleuropneumoniae in mice and pigs","volume":"124","author":"Wu","year":"2018","journal-title":"J Appl Microbiol"},{"key":"2026073010425269300_bib44","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1111\/jam.14874","article-title":"Immunogenicity and protective ability of RpoE against Streptococcus suis serotype 2","volume":"130","author":"Yi","year":"2021","journal-title":"J Appl Microbiol"},{"key":"2026073010425269300_bib45","doi-asserted-by":"publisher","first-page":"4672","DOI":"10.1016\/j.vaccine.2014.06.002","article-title":"Evaluation of a multisubunit recombinant polymorphic membrane protein and major outer membrane protein T cell vaccine against Chlamydia muridarum genital infection in three strains of mice","volume":"32","author":"Yu","year":"2014","journal-title":"Vaccine"},{"key":"2026073010425269300_bib46","doi-asserted-by":"publisher","first-page":"38577","DOI":"10.1038\/srep38577","article-title":"LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against Vibrio species infection","volume":"6","author":"Zha","year":"2016","journal-title":"Sci Rep"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxag129\/68455715\/lxag129.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/6\/lxag129\/68455715\/lxag129.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/6\/lxag129\/68455715\/lxag129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:43:06Z","timestamp":1785422586000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxag129\/8700179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxag129","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,6]]},"published":{"date-parts":[[2026,6]]},"article-number":"lxag129"}}