{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:05:45Z","timestamp":1785423945669,"version":"3.56.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aimed to determine whether heat\u2013killed Latilactobacillus curvatus K4G4 exerts anti\u2013inflammatory effects in vivo using a monosodium urate\u2013induced peritonitis mouse model, and to elucidate the underlying mechanisms with a focus on IL\u201310-mediated regulation of NLRP3 inflammasome activation in macrophages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>C57BL\/6 J mice were fed K4G4\u2013supplemented diets for 2\u202fweeks prior to monosodium urate challenge. K4G4 significantly reduced monosodium urate\u2013induced neutrophil infiltration and suppressed the expression of Il1b and Nlrp3 in peritoneal cells. Spleen IL\u201310 levels were elevated in K4G4\u2013fed mice. In J774.1 cells, K4G4 pretreatment increased Il10 expression and IL\u201310 secretion, and dose\u2013dependently inhibited LPS\/nigericin\u2013induced IL\u20131\u03b2 production and LDH release. IL\u201310 neutralization restored IL\u20131\u03b2 production, indicating IL-10-dependent suppression of inflammasome activation. K4G4 also inhibited the priming step by reducing LPS\u2013induced Il1b, Tnfa, and Nlrp3 transcription and suppressing NF\u2013\u03baB nuclear translocation. Furthermore, K4G4 attenuated LPS\/nigericin\u2013induced caspase\u20131 activation, confirming inhibition of the activation step.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Heat-killed K4G4 suppresses MSU-induced peritonitis in vivo and is associated with inhibition of both the priming and activation steps of NLRP3 inflammasome signaling, potentially involving IL-10\u2013dependent mechanisms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag132","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:30:26Z","timestamp":1780399826000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Latilactobacillus curvatus<\/i>\n                    K4G4 suppresses NLRP3 inflammasome activation via IL\u201310 induction and ameliorates monosodium urate-induced peritonitis"],"prefix":"10.1093","volume":"137","author":[{"given":"Kazuha","family":"Yamada","sequence":"first","affiliation":[{"name":"Obihiro University of Agriculture and Veterinary Medicine Department of Life and Food Sciences, , Obihiro 080-8555 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aki","family":"Okano","sequence":"additional","affiliation":[{"name":"Obihiro University of Agriculture and Veterinary Medicine Department of Life and Food Sciences, , Obihiro 080-8555 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9797-1705","authenticated-orcid":false,"given":"Sachi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Academic Assembly (Institute of Agriculture), Shinshu University , Nagano 399-4598 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