{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:57:11Z","timestamp":1791946631087,"version":"4.3.4"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To evaluate whether the cell-free supernatant (CFS) of a poultry-derived Lactiplantibacillus sp. strain PCE3 can inhibit biofilm biomass accumulation and hyphal morphogenesis of Candida albicans ATCC 10231 in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Biofilm biomass was quantified by crystal violet staining after 48 h growth in RPMI 1640 plus 2% glucose with 10%, 20%, or 40% (v\/v) CFS. Filamentation was induced in RPMI 1640 plus 10% fetal bovine serum at 37 \u00b0C and assessed qualitatively by light microscopy, scanning electron microscopy, and representative 24 h microscopy observations. Planktonic growth was summarized by 24-h colony-forming unit counts. CFS inhibited biofilm biomass in a concentration-dependent manner, with mean inhibition of 29.6 \u00b1 8.3%, 48.1 \u00b1 7.2%, and 79.8 \u00b1 5.8% at 10%, 20%, and 40% (v\/v), respectively (all\u00a0p &amp;lt; 0.05 vs control). Under hypha-inducing conditions, 40% CFS shifted morphology from extensive filamentation in untreated controls to predominantly moderate or suppressed filamentation, and representative microscopy supported persistence of this non-filamentous phenotype over the observation period. Planktonic viable counts decreased by up to ~1.1 log10 at 40% CFS, indicating partial growth inhibition without eradication.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Poultry-derived Lactiplantibacillus sp. strain PCE3 CFS showed in vitro anti-virulence activity against C. albicans, reducing biofilm biomass and restraining filamentation more strongly than planktonic growth. These findings support avian-derived postbiotic preparations as an underexplored source of anti-Candida activity for further characterization and validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag142","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T11:28:53Z","timestamp":1781004533000},"source":"Crossref","is-referenced-by-count":1,"title":["Poultry-derived\n                    <i>Lactiplantibacillus<\/i>\n                    sp. PCE3 cell-free supernatant inhibits\n                    <i>Candida albicans<\/i>\n                    biofilm biomass and hyphal morphogenesis\n                    <i>in vitro<\/i>"],"prefix":"10.1093","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3320-4072","authenticated-orcid":false,"given":"Dang Anh","family":"Tuan","sequence":"first","affiliation":[{"name":"Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technick\u00e1 5, 166 28 Prague 6 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6484-0824","authenticated-orcid":false,"given":"Jan","family":"Masak","sequence":"additional","affiliation":[{"name":"Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technick\u00e1 5, 166 28 Prague 6 ,","place":["Czech 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