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Acrocalymma and Setophoma terrestris were the most commonly isolated DSE species, but strains with lower isolation rates Paraphoma ledniceana, Amesia atrobrunnea, and Zopfiella pilifera exhibited the most pronounced positive effects on yam root biomass. Increasing the number of co-inoculated strains did not proportionally enhance yam growth but progressively restructured soil microbial communities. However, mixed DSE inoculation as a whole showed no obvious growth promotion compared with the control. Fungal co-occurrence networks exhibited higher modularity and clustering, while bacterial communities had greater connectivity, especially under P. ledniceana and Fusarium sp. inoculation. Biosynthesis was the most abundant predicted function of rhizosphere microbes, with bacteria predicted to be enriched in detoxification and fungi in degradation-related pathways. Two strains of Chaetomiaceae and Periconia epilithographicola increased soil pH, organic carbon and available phosphorus, while available nitrogen decreased after DSE inoculation. Importantly, P. epilithographicola inoculation enhanced mycorrhizal fungi and mitigated microbial imbalance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Single and mixed DSE inoculations regulate rhizosphere microbial assembly and nutrient cycling via species-specific effects. Screening beneficial DSE consortia and characterizing their influence on rhizosphere networks provides preliminary theoretical insights and and candidate strains that may relieve yam replant obstacles in future applications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag165","type":"journal-article","created":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T11:33:40Z","timestamp":1783251220000},"source":"Crossref","is-referenced-by-count":0,"title":["Preliminary strain\u2013specific and non\u2013additive effects of single versus mixed DSE inoculation on rhizosphere microbiome and nutrient cycling relative to plant biomass in Chinese yam"],"prefix":"10.1093","volume":"137","author":[{"given":"Zihang","family":"Su","sequence":"first","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6919-2408","authenticated-orcid":false,"given":"Yiling","family":"Zuo","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]},{"name":"Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengci","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Hebei University , Baoding, Hebei 071000 ,","place":["China"]},{"name":"Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Hebei University , Baoding, Hebei 071000 ,","place":["China"]},{"name":"Hebei Administration of Traditional Chinese Medicine Collaborative Innovation Key Research Laboratory of Chinese Medicinal Resource Industrialization , Baoding, Hebei 071000 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"2026092506480050100_bib1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5943\/mycosphere\/14\/1\/1","article-title":"Endophytic fungi in green manure crops; friends or foe","volume":"14","author":"Abeywickrama","year":"2023","journal-title":"Mycosphere"},{"key":"2026092506480050100_bib2","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.tim.2023.03.002","article-title":"Microbial drought resistance may destabilize soil carbon","volume":"31","author":"Allison","year":"2023","journal-title":"Trends Microbiol"},{"key":"2026092506480050100_bib3","doi-asserted-by":"publisher","first-page":"117589","DOI":"10.1016\/j.lwt.2025.117589","article-title":"Metabolomic analysis reveals mechanisms linking metabolic shifts and characteristic biomarkers to quality changes in Dioscorea opposita Thunb. cv. 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