{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:49:58Z","timestamp":1792799398714,"version":"4.5.0"},"reference-count":149,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Geran Putra Inisiatif","award":["GPI\/2023\/9764700"],"award-info":[{"award-number":["GPI\/2023\/9764700"]}]},{"name":"Prototype Development Research Grant","award":["PRGS\/1\/2023\/STG02\/USM\/02\/3)"],"award-info":[{"award-number":["PRGS\/1\/2023\/STG02\/USM\/02\/3)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Bacterial infections that cause acute systemic infection are the leading cause of health issues globally. Current diagnostic assays for bacterial infection include culture and nucleic acid amplification methods. However, the culture method is very time-consuming and expensive, while the nucleic acid amplification method, although it is sensitive, requires trained personnel. Aptamer-based biosensors are promising alternatives to these strategies owing to aptamers\u2019 advantages: cost-saving, no batch-to-batch variation, and high specificity and sensitivity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>In view of their diagnostic potential, we aim to conduct a systematic review on various biosensors harnessed for the diagnosis of bacteria, which was performed using spiked and clinical samples between 2018 and 2026.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Based on the specific inclusion and exclusion criteria, the studies performed in recent years (2018\u201326) were filtered from two databases, PubMed and Scopus. The inclusion criteria were aptamer-based biosensors for the detection of bacteria in spiked and clinical samples from patients with human infectious diseases. A total of 42 studies were included in this systematic review that involve aptamer-based detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Mycobacterium tuberculosis, Acinetobacter baumanii, Pseudomonas aeruginosa, Neisseria meningitidis, Streptococcus pneumoniae, Salmonella typhimurium, Salmonella enterica, Helicobacter pylori, Roseburia intestinalis, Group B Streptococcus, and Haemophilus influenzae.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Aptamer-based biosensors show immense prospects for the diagnostics of pathogenic bacteria.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag189","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:19:54Z","timestamp":1785151194000},"source":"Crossref","is-referenced-by-count":1,"title":["Aptamer-based biosensors for the diagnostics of infectious disease-causing bacteria using spiked and clinical samples: a systematic review"],"prefix":"10.1093","volume":"137","author":[{"given":"Nurul Farzana","family":"Ismail","sequence":"first","affiliation":[{"name":"Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia , 43400 Serdang, Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marimuthu","family":"Citartan","sequence":"additional","affiliation":[{"name":"Pusat Kanser Tun Abdullah Ahmad Badawi (PKTAAB), Universiti Sains Malaysia , Bertam, 13200 Kepala Batas, Penang ,","place":["Malaysia"]},{"name":"School of Biotechnology, MILA University , No 1, MIU Boulevard, Putra Nilai, 71800 Nilai, Negeri Sembilan ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurul Asyikin","family":"Mahbud","sequence":"additional","affiliation":[{"name":"Universiti Putra Malaysia Department of Medical Microbiology, Faculty of Medicine and Health Sciences, , 43400 Serdang, Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choo Yee","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia , 43400 Serdang, Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharifah Sakinah Syed","family":"Alwi","sequence":"additional","affiliation":[{"name":"Universiti Putra Malaysia Department of Biomedical Science, Faculty of Medicine and Health Sciences, , 43400 Serdang, Selangor ,","place":["Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisse Mary Sither","family":"Joseph","sequence":"additional","affiliation":[{"name":"Universiti Putra Malaysia Department of Medical Microbiology, Faculty of Medicine and Health Sciences, , 43400 Serdang, Selangor 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