{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T15:01:18Z","timestamp":1793977278803,"version":"4.8.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the antimicrobial resistance, \u03b2-lactamase genes, biofilm formation, and genomic characteristics of Klebsiella species isolated from diseased dogs in southeastern Brazil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fifty-five Klebsiella isolates from dogs with clinical infections were identified by MALDI\u2012TOF MS. Antimicrobial susceptibility was assessed by disk diffusion. ESBL and carbapenemase genes were detected by PCR. Biofilm formation was evaluated using a microtiter plate assay. The only blaKPC-positive isolate underwent whole-genome sequencing and phylogenetic analysis with publicly available Brazilian genomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Klebsiella pneumoniae was the predominant species (74.5%), followed by K. variicola (20%). Multidrug resistance was observed in 36.4% of the K. pneumoniae isolates. High resistance rates of the isolates were observed for fluoroquinolones, cephalosporins, and sulfonamides, whereas chloramphenicol showed moderate susceptibility. The most frequent \u03b2-lactamase genes were blaTEM-1 (34.5%) and blaCTX-M-15 (30.9%). A single isolate (1.8%) harbored blaKPC. No hypervirulence-associated genes were detected. All the isolates formed biofilms, predominantly with weak to moderate adhesion. Whole-genome sequencing of the blaKPC-positive isolate revealed K. pneumoniae ST11 (CG340) carrying multiple resistance genes, including blaKPC-2, and clustering with human-associated genomes within a high-risk lineage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Multidrug-resistant K. pneumoniae, including a high-risk ST11 blaKPC-2-producing clone, was identified in diseased dogs, supporting the need to investigate companion animals as possible reservoirs of clinically relevant antimicrobial resistance determinants. The combination of multidrug resistance and biofilm formation highlights the importance of continued surveillance and prudent antimicrobial use within One Health.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag198","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T12:03:42Z","timestamp":1785585822000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>Klebsiella<\/i>\n                    species isolated from diseased dogs in Brazil reveal multidrug resistance, CTX-M-15 predominance and detection of a KPC-2-producing ST11\n                    <i>Klebsiella pneumoniae<\/i>\n                    isolate"],"prefix":"10.1093","volume":"137","author":[{"given":"Patrik J\u00fanior de Lima","family":"Paz","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Spessotto","family":"Bello","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Fagali","family":"Arabe-Filho","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Vin\u00edcius Ramos","family":"Portilho","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanoelli Aparecida","family":"Rodrigues\u00a0dos\u00a0Santos","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Henrique","family":"Camargo","sequence":"additional","affiliation":[{"name":"Adolfo Lutz Institute (IAL), S\u00e3o Paulo Regional Laboratory , S\u00e3o Paulo, S\u00e3o Paulo CEP 01246-000 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera L\u00facia M\u00f3res","family":"Rall","sequence":"additional","affiliation":[{"name":"Institute of Biosciences, UNESP , Botucatu, S\u00e3o Paulo CEP 18618-689 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano Gon\u00e7alves","family":"Pereira","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, S\u00e3o Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine, , Botucatu, S\u00e3o Paulo CEP 18618-681 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rog\u00e9rio","family":"Giuffrida","sequence":"additional","affiliation":[{"name":"University of Western S\u00e3o Paulo\u2014Unoeste , Presidente Prudente, S\u00e3o Paulo CEP 19067-175 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcio Garcia","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine 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