{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:10:47Z","timestamp":1790334647624,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K01701"],"award-info":[{"award-number":["24K01701"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K22205"],"award-info":[{"award-number":["24K22205"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Campylobacter jejuni and Campylobacter coli are leading causes of foodborne illnesses, including human enteritis and diarrhea, in many countries. The prevalence of antibiotic-resistant Campylobacter is increasing in both developing and developed countries, with fluoroquinolone-resistant Campylobacter recognized as a priority pathogen among antibiotic-resistant bacteria. Surveillance of Campylobacter antimicrobial resistance (AMR) is conducted worldwide because of its public health importance. In this study, we characterized the antimicrobial resistance patterns and genomic diversity of C. jejuni and C. coli strains isolated from food handlers in Japan.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Surveillance was conducted on 106 Campylobacter isolates from food handlers between 2008 and 2023. Species identification by 16S rRNA sequencing, antimicrobial susceptibility testing, and whole-genome sequencing of selected isolates were performed to characterize phenotypic and genotypic features, compare AMR profiles, and assess phylogeny. Multilocus sequence typing (MLST) identified 36 sequence types (STs), including three novel STs and 10 clonal complexes (CCs). Among\u00a0C. jejuni, CC21 (20.6%) was the most prevalent, followed by CC353 (2.8%). In C. coli, CC828 (4.7%) was the most prevalent. The AMR profiles and resistance determinants were as follows: quinolones (68.2%); mutations in the quinolone resistance-determining region (gyrA T86I); tetracyclines (40.2%); acquisition of tet(O) and macrolides (2.8%); and mutations in the 23S rRNA gene (A2075G). ST4526 (8.4%), reported only in Japan, was the most prevalent; however, it has not been detected since 2021, suggesting recent ST diversification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study characterized the AMR profiles and genetic backgrounds of non-clinical human-derived Campylobacter strains isolated from food handlers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag199","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:42:42Z","timestamp":1785843762000},"source":"Crossref","is-referenced-by-count":0,"title":["Clonal diversity and shifting lineages of multidrug-resistant\n                    <i>Campylobacter<\/i>\n                    among healthy food handlers in Japan"],"prefix":"10.1093","volume":"137","author":[{"given":"Kairi","family":"Miyaji","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima, Hiroshima 739-8528 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsuki","family":"Ohata","sequence":"additional","affiliation":[{"name":"Kobe Institute of Health Health Research Department 2, , Kobe, Hyogo 650-0046 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamoru","family":"Noda","sequence":"additional","affiliation":[{"name":"Machida Hygienic Control Laboratory , Machida, Tokyo 194-0013 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritaka","family":"Hatta","sequence":"additional","affiliation":[{"name":"Machida Hygienic Control Laboratory , Machida, Tokyo 194-0013 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Graduate School of Integrated Sciences for Life, Hiroshima University , Higashi-Hiroshima, Hiroshima 739-8528 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"2026092506473211800_bib1","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1086\/319760","article-title":"Campylobacter jejuni infections: update on emerging issues and trends","volume":"32","author":"Acheson","year":"2001","journal-title":"Clin Infect Dis"},{"key":"2026092506473211800_bib2","doi-asserted-by":"publisher","first-page":"e48394","DOI":"10.1371\/journal.pone.0048394","article-title":"Molecular evidence for the thriving of Campylobacter jejuni ST-4526 in Japan","volume":"7:","author":"Asakura","year":"2012","journal-title":"PLoS 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