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To evaluate the contribution of the intestinal microbiota, fecal microbiota transplantation (FMT) was performed using microbiota from hypoxia-exposed or normoxic donor mice into antibiotic-treated recipients before exhaustive exercise under hypobaric hypoxia. Compared with normoxic controls, hypoxia-exposed mice exhibited reduced endurance, increased oxidative stress, impaired intestinal barrier function, decreased Ruminococcus abundance, and significant alterations in both \u03b1- and \u03b2-diversity. Recipient mice receiving microbiota from hypoxia-exposed donors developed similar phenotypes, including greater oxidative stress, reduced barrier protein expression, and microbial community profiles resembling those of hypoxic donors. Comparisons with microbiota-depleted controls further indicated that gut microbial alterations contributed to, but were not sufficient alone to cause, impaired exercise performance in the absence of the hypoxic environment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Hypobaric hypoxia rapidly remodels the intestinal microbiota, and these microbial alterations contribute to oxidative stress, intestinal barrier dysfunction, and reduced exercise capacity under hypoxic conditions. FMT supports a contributory role of the intestinal microbiota in hypoxia-associated physiological dysfunction while indicating that hypoxic stress remains necessary for the full fatigue phenotype to develop. These findings support further investigation of microbiota-targeted strategies to mitigate exercise impairment during hypobaric hypoxia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag217","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T13:10:48Z","timestamp":1788181848000},"source":"Crossref","is-referenced-by-count":0,"title":["Microbial dysbiosis exacerbates exercise fatigue in mice under hypobaric hypoxia"],"prefix":"10.1093","volume":"137","author":[{"given":"Guanwen","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Science and Technology, Northwestern Polytechnical University , 127 Youyi West Road, Xi\u2019an, Shaanxi Province 710072 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Northwestern Polytechnical University , 127 Youyi West Road, 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