{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:55:36Z","timestamp":1790700936563,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dutch National Institute"},{"DOI":"10.13039\/100018693","name":"Horizon Europe","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"DGAPA","doi-asserted-by":"publisher","award":["101135241"],"award-info":[{"award-number":["101135241"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biological containment strategies are widely used to reduce possible risks associated with genetically modified microorganisms (GMMs). In the biosafety literature, the performance of these systems is often benchmarked against a maximum escape frequency of one cell per 108 cells. This value is commonly attributed to the USA National Institutes of Health Guidelines for Research Involving Recombinant DNA. However, the guideline refers specifically to laboratory certification of certain host-vector systems and does not define an acceptable escape frequency for applications outside controlled laboratory environments. Despite this limited scope, the 10\u20138 criterion has been repeatedly cited in research articles and reviews as a general biosafety standard for GMMs. At the same time, quantitative data on escape frequency and survival of genetically modified microorganisms under realistic environmental conditions remain scarce. This lack of empirical evidence complicates environmental risk assessment and can hinder regulatory approval and technology transfer for applications intended to operate beyond the laboratory. Here, we clarify the origin and scope of the 10\u20138 escape criterion and discuss why it should not be interpreted as a universal biosafety standard. We argue that experimentally validated measurements of escape and survival under application-relevant conditions are urgently needed to support evidence-based biosafety assessment and the responsible development and deployment of GMMs.<\/jats:p>","DOI":"10.1093\/jambio\/lxag227","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:00:04Z","timestamp":1788440404000},"source":"Crossref","is-referenced-by-count":0,"title":["Rethinking escape frequency benchmarks for environmental applications of engineered microorganisms"],"prefix":"10.1093","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2171-5302","authenticated-orcid":false,"given":"Sonia","family":"Mecacci","sequence":"first","affiliation":[{"name":"Bioprocess Engineering Group, Wageningen University and Research , Wageningen 6700 AA ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2352-9017","authenticated-orcid":false,"given":"Vitor A P","family":"Martins\u00a0dos\u00a0Santos","sequence":"additional","affiliation":[{"name":"Bioprocess Engineering Group, Wageningen University and Research , Wageningen 6700 AA ,","place":["The Netherlands"]},{"name":"LifeGlimmer GmbH , Berlin 12163 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-345X","authenticated-orcid":false,"given":"Enrique","family":"Asin-Garcia","sequence":"additional","affiliation":[{"name":"Bioprocess Engineering Group, Wageningen University and Research , Wageningen 6700 AA ,","place":["The 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