{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:56:07Z","timestamp":1790700967024,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["n\u00b0 2018\/12458-5"],"award-info":[{"award-number":["n\u00b0 2018\/12458-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To address the severe defoliation caused by the invasive bronze bug, Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae), this study investigated the mass production potential of three entomopathogenic fungal (EPF) strains and the specific biocontrol efficacy of Metarhizium anisopliae against this pest.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Initially, Cordyceps javanica (LCBPF 11), M. anisopliae (LCBPF 31), and Beauveria bassiana (LCBPF 46) produced\u00a0&amp;gt;\u00a01\u00a0\u00d7\u00a010\u2079 conidia g\u207b\u00b9 in solid-state fermentation. To scale production, liquid culture was selected for C. javanica and M. anisopliae using industrial by-products (corn flour and molasses), while M. anisopliae was further compared in a rice flour-dextrose-yeast extract (M1) medium. Fitness assays revealed that while M. anisopliae blastospores (M1) were more heat-susceptible at 45\u00b0C (LT50 0.182\u00a0h) than aerial conidia (LT50 1.621\u00a0h), survival analysis of bioassays against T. peregrinus adults revealed highly significant differences among treatments. Remarkably, all M. anisopliae propagules achieved 100% mortality within 5 days, with blastospores produced in M1 medium reaching an LT50 of only 1.81 days. Furthermore, Cox proportional hazards modeling confirmed that these optimized blastospores (M1) presented a mortality risk 2.51 times higher than the commercial standard Metarril\u00ae based on aerial conidia (Hazard Ratio = 2.51; 95% CI: 1.90\u20133.31).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>To the best of our knowledge, this is the first report of M. anisopliae blastospores successfully infecting T. peregrinus. Taken altogether, these findings demonstrate the technical feasibility and significant potential of these rapid-acting infective propagules for the management of this forest pest.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag236","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:48:25Z","timestamp":1789127305000},"source":"Crossref","is-referenced-by-count":0,"title":["Production of Entomopathogenic Fungi and Pathogenicity of\n                    <i>Metarhizium anisopliae<\/i>\n                    against\n                    <i>Thaumastocoris peregrinus<\/i>\n                    (Hemiptera: Thaumastocoridae)"],"prefix":"10.1093","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-506X","authenticated-orcid":false,"given":"Thiago Souza","family":"Bulh\u00f5es","sequence":"first","affiliation":[{"name":"Laboratory of Biological Control of Forest Pests, Department of Plant Protection, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-5031","authenticated-orcid":false,"given":"Paula Leite","family":"dos\u00a0Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Control of Forest Pests, Department of Plant Protection, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8867-8470","authenticated-orcid":false,"given":"Bruno Felipe Prado","family":"de\u00a0Melo","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Control of Forest Pests, Department of Plant Protection, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2229-464X","authenticated-orcid":false,"given":"Vanessa Rafaela","family":"de\u00a0Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Control of Forest Pests, Department of Plant Protection, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3394-9178","authenticated-orcid":false,"given":"Bianca Cristina Costa","family":"G\u00eaa","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Control of Forest Pests, Department of Plant Protection, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4119-8642","authenticated-orcid":false,"given":"Maria M\u00e1rcia Pereira","family":"Sartori","sequence":"additional","affiliation":[{"name":"Department of Plant Production, School of Agricultural Sciences (FCA), S\u00e3o Paulo State University (UNESP) , 18610-034, Botucatu, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3517-6826","authenticated-orcid":false,"given":"Gabriel Moura","family":"Mascarin","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation, Embrapa Environment , Rodovia SP-340, km 127.5, 13918-110, Jaguari\u00fana, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9875-4158","authenticated-orcid":false,"given":"Carlos 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