{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:06:50Z","timestamp":1791378410372,"version":"4.3.2"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274282"],"award-info":[{"award-number":["82274282"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2022JJ30437"],"award-info":[{"award-number":["2022JJ30437"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstracts<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Slow transit constipation (STC) is a common functional gastrointestinal disorder characterized by intestinal motility dysfunction, barrier damage, hormonal imbalance, and gut microbiota dysbiosis. High-concentration (50%) lactulose is routinely used for STC treatment, while Bacillus pumilus acts as a probiotic to regulate intestinal flora. However, the synergistic efficacy and mechanism of low-dose lactulose combined with Bacillus pumilus NJAUYBN1-7 for STC remain unclear. This study aimed to clarify the therapeutic effects and underlying mechanisms of this combined therapy and compare its efficacy with conventional high-dose lactulose monotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>A loperamide-induced mouse STC model was applied for efficacy evaluation. Compared with 50% lactulose monotherapy, 30% lactulose combined with vegetative cells of Bacillus pumilus NJAUYBN1-7 (2.0\u00a0\u00d7\u00a0109 CFU\u00b7mL\u22121) significantly relieved constipation symptoms and shortened the first defecation time to 174.5 \u00b1 9.14 min. Meanwhile, fecal output, fecal water content (52.08% \u00b1 5.33%), and intestinal transit rate (49.38% \u00b1 2.46%) were markedly increased. Mechanistically, the combination restored the SCF\/Kit pathway, significantly upregulated the expression of intestinal barrier-related proteins (P &amp;lt; 0.05), normalized gastrointestinal hormone concentrations, alleviated colonic inflammation by reducing pro-inflammatory cytokine levels (P &amp;lt; 0.05), and remodeled gut microbiota homeostasis by altering the relative abundance of key enteric bacteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Thirty percent of lactulose combined with\u00a0Bacillus pumilus NJAUYBN1-7 exhibits superior STC therapeutic effects compared with 50% lactulose monotherapy. This synergistic regimen improves intestinal motility, repairs intestinal barrier, relieves inflammation, and optimizes intestinal microenvironment via multi-target regulation. This study provides novel mechanistic insights and validates the clinical translational potential of this safe and efficient combined strategy for STC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag244","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:00:15Z","timestamp":1790769615000},"source":"Crossref","is-referenced-by-count":0,"title":["Alleviative effects of low-dose lactulose combined with\n                    <i>Bacillus pumilus<\/i>\n                    NJAUYBN1-7 on slow transit constipation in loperamide-induced mice"],"prefix":"10.1093","volume":"137","author":[{"given":"Youdan","family":"Sun","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Institute of Animal Nutritional Health, Nanjing Agricultural University , Nanjing 210095, Jiangsu Province 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