{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T16:39:52Z","timestamp":1792600792181,"version":"4.5.0"},"reference-count":113,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100008697","name":"University of Karachi","doi-asserted-by":"publisher","award":["3240325075"],"award-info":[{"award-number":["3240325075"]}],"id":[{"id":"10.13039\/100008697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study presents an exploratory assessment of volatile and semi-volatile metabolites produced by endophytic fungi associated with Moringa oleifera using gas chromatography\u2013mass spectrometry (GC-MS), emphasizing metabolite occurrence and analytical consistency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>Twelve endophytic fungal isolates were recovered from leaf and twig tissues and putatively identified by morphology and internal transcribed spacer (ITS) sequencing. Each isolate was cultivated as a single, unreplicated 1 l shake-flask culture in Sabouraud dextrose broth (28\u00b0C, 120 rpm, 12\u201320 days), and the cell-free broth extracted with an equal volume of n-hexane. Initial spectral matching generated a broad set of compound annotations. Curation removed analytical artefacts, contaminants (including siloxanes and phthalates), and low-confidence identifications, leaving a small set of biologically plausible, tentatively identified metabolites, among them furfuryl alcohol, kojic acid, lauric acid, palmitic acid, and undecylenic acid. Cross-instrument comparison on two GC-MS platforms revealed substantial variability when instrument-specific libraries were used; commercial library matching returned twelve tentatively identified metabolites in one extract and six in the other on the second platform, and none on the first. No compound was recovered on both platforms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Metabolite annotation proved highly sensitive to instrument configuration and to the choice of spectral library: the same extracts yielded entirely non-overlapping compound lists on two platforms from the same manufacturer using the same detector type and ionization energy. These findings highlight the importance of stringent data curation, cautious spectral interpretation, and analytical standardization in GC-MS-based metabolomics. All assignments are tentative, based on library matching without co-injection of authentic standards.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jambio\/lxag249","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:40:06Z","timestamp":1791286806000},"source":"Crossref","is-referenced-by-count":0,"title":["Data curation and cross-instrument variability influence GC-MS-based metabolite profiling of endophytic fungi associated with\n                    <i>Moringa oleifera<\/i>"],"prefix":"10.1093","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6175-6387","authenticated-orcid":false,"given":"Markwo","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Ghana , P.O. Box LG 56, Legon, Accra ,","place":["Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekri Melka","family":"Abdo","sequence":"additional","affiliation":[{"name":"Wondo Genet Natural Product Research Laboratory, Wondo Genet Agricultural Research Center, Ethiopian Institute of Agricultural Research , P.O. Box 198, Shashemene ,","place":["Ethiopia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salar","family":"Hafez-Ghoran","sequence":"additional","affiliation":[{"name":"H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi , Karachi 75270 ,","place":["Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorcas","family":"Osei-Safo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Ghana , P.O. Box LG 56, Legon, Accra ,","place":["Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Kingsford-Adaboh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Ghana , P.O. Box LG 56, Legon, Accra ,","place":["Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Atia-tul\u00a0Wahab","sequence":"additional","affiliation":[{"name":"Dr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi , Karachi 75270 ,","place":["Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Iqbal","family":"Choudhary","sequence":"additional","affiliation":[{"name":"H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi , Karachi 75270 ,","place":["Pakistan"]},{"name":"Dr. Panjwani Center for Molecular Medicine and Drug Research, ICCBS, University of Karachi , Karachi 75270 ,","place":["Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"2026102111491985700_bib1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.21608\/EJMM.2021.197273","article-title":"Enhancement of taxol production by endophytic fungi from Hibiscus and Moringa plant using gamma irradiation","volume":"30","author":"Abdel-Fatah","year":"2021","journal-title":"Egypt J Med Microbiol"},{"key":"2026102111491985700_bib2","doi-asserted-by":"publisher","DOI":"10.1016\/C2021-0-02807-8","volume-title":"Bacterial Secondary Metabolites: Synthesis and Applications in Agroecosystem","author":"Abd-Elsalam","year":"2023"},{"key":"2026102111491985700_bib3","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s00425-020-03468-1","article-title":"Secondary metabolites as plant defensive strategy: a large role for small molecules in the near root region","volume":"252","author":"Adedeji","year":"2020","journal-title":"Planta"},{"key":"2026102111491985700_bib4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13065-016-0155-2","article-title":"Freeze-drying: an alternative method for the analysis of volatile organic compounds in the headspace of urine samples using solid phase micro-extraction coupled to gas chromatography\u2013mass spectrometry","volume":"10","author":"Aggio","year":"2016","journal-title":"Chem Cent J"},{"key":"2026102111491985700_bib5","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/978-3-030-54027-2_5","article-title":"Role of stress and defense in plant secondary metabolites production","volume":"140","author":"Aguirre-Becerra","year":"2021","journal-title":"Adv Struct Mater"},{"key":"2026102111491985700_bib6","doi-asserted-by":"publisher","first-page":"295","DOI":"10.33804\/pp.007.02.4741","article-title":"Unveiling the pathogenic and beneficial fungi in potato farming of Gilgit Baltistan, Pakistan","volume":"7","author":"Ahmad","year":"2023","journal-title":"Plant Prot"},{"key":"2026102111491985700_bib7","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.mmcr.2013.07.001","article-title":"Identification of Curvularia lunata by polymerase chain reaction in a case of fungal endophthalmitis","volume":"2","author":"Alex","year":"2013","journal-title":"Med Mycol Case Rep"},{"key":"2026102111491985700_bib8","doi-asserted-by":"publisher","first-page":"716","DOI":"10.3390\/metabo13060716","article-title":"Plant secondary metabolites: the weapons for biotic stress management","volume":"13","author":"Al-Khayri","year":"2023","journal-title":"Metabolites"},{"key":"2026102111491985700_bib9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ijms21175958","article-title":"Diversity and antimicrobial activity of endophytic fungi isolated from Chloranthus japonicus Sieb. in Qinling Mountains, China","volume":"21","author":"An","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2026102111491985700_bib10","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1007\/978-3-031-15130-9_123","article-title":"Mycological examination","volume-title":"European Handbook of Dermatological Treatments","author":"Atzori","year":"2023"},{"key":"2026102111491985700_bib11","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/s12866-017-0958-x","article-title":"Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)","volume":"17","author":"Badotti","year":"2017","journal-title":"BMC Microbiol"},{"key":"2026102111491985700_bib12","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1186\/1471-2180-10-189","article-title":"ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases","volume":"10","author":"Bellemain","year":"2010","journal-title":"BMC Microbiol"},{"key":"2026102111491985700_bib13","doi-asserted-by":"publisher","first-page":"933017","DOI":"10.3389\/fmicb.2022.933017","article-title":"Endophytes and their potential in biotic stress management and crop production","volume":"13","author":"Chaudhary","year":"2022","journal-title":"Front Microbiol"},{"key":"2026102111491985700_bib14","doi-asserted-by":"publisher","first-page":"e58268","DOI":"10.1371\/journal.pone.0058268","article-title":"Diversity and taxonomy of endophytic Xylariaceous fungi from medicinal plants of Dendrobium (Orchidaceae)","volume":"8","author":"Chen","year":"2013","journal-title":"PLoS One"},{"key":"2026102111491985700_bib15","doi-asserted-by":"publisher","first-page":"2111","DOI":"10.1007\/s00253-023-12451-1","article-title":"Fungal production of kojic acid and its industrial applications","volume":"107","author":"Chib","year":"2023","journal-title":"Appl Microbiol Biotechnol"},{"key":"2026102111491985700_bib16","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1007\/978-3-031-49112-2_14","article-title":"Endophytic fungi and the health benefits from their potential bioactive secondary metabolites","volume-title":"Endophytic Fungi: The Hidden Sustainable Jewels for the Pharmaceutical and Agricultural Industries","author":"Das","year":"2024"},{"key":"2026102111491985700_bib17","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/s13225-013-0229-6","article-title":"Diversity and delimitation of Rhizopus microsporus","volume":"64","author":"Dolatabadi","year":"2014","journal-title":"Fungal Divers"},{"key":"2026102111491985700_bib18","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1186\/s12866-023-02958-y","article-title":"Identification and pathogenicity analysis of Fusarium spp. on peach in China","volume":"23","author":"Dong","year":"2023","journal-title":"BMC Microbiol"},{"key":"2026102111491985700_bib19","doi-asserted-by":"publisher","first-page":"1044896","DOI":"10.3389\/fpls.2022.1044896","article-title":"The hidden power of secondary metabolites in plant-fungi interactions and sustainable phytoremediation","volume":"13","author":"Elhamouly","year":"2022","journal-title":"Front Plant Sci"},{"key":"2026102111491985700_bib20","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1111\/lam.13345","article-title":"Diversity of endophytic fungi from the leaves of Vaccinium dunalianum","volume":"71","author":"Fan","year":"2020","journal-title":"Lett Appl Microbiol"},{"key":"2026102111491985700_bib21","doi-asserted-by":"publisher","first-page":"75","DOI":"10.3390\/antibiotics9020075","article-title":"Antimicrobial activity of host-derived lipids","volume":"9","author":"Fischer","year":"2020","journal-title":"Antibiotics"},{"key":"2026102111491985700_bib22","first-page":"34286","article-title":"Morphological characteristics of some microfungal species isolated from spontaneously fermented Jatropha curcas seed meal","volume":"85","author":"Gonu","year":"2015","journal-title":"Elixir Microbiol Ferment"},{"key":"2026102111491985700_bib23","doi-asserted-by":"publisher","first-page":"71","DOI":"10.26757\/pjsb2019a13008","article-title":"Siderophores of fungi from Lipa clay loam soil, Philippines","volume":"13","author":"Guerrero","year":"2020","journal-title":"Philipp J Syst Biol"},{"key":"2026102111491985700_bib24","doi-asserted-by":"publisher","first-page":"188","DOI":"10.3390\/toxins14030188","article-title":"The potential of fatty acids and their derivatives as antifungal agents: a review","volume":"14","author":"Guimar\u00e3es","year":"2022","journal-title":"Toxins"},{"key":"2026102111491985700_bib25","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s11046-018-0299-9","article-title":"The molecular identification and antifungal susceptibilities of Aspergillus species causing otomycosis in Tochigi, Japan","volume":"184","author":"Hagiwara","year":"2019","journal-title":"Mycopathologia"},{"key":"2026102111491985700_bib26","doi-asserted-by":"publisher","first-page":"109955","DOI":"10.1016\/j.ijfoodmicro.2022.109955","article-title":"Volatile organic compounds mediated endogenous microbial interactions in Chinese baijiu fermentation","volume":"383","author":"Hao","year":"2022","journal-title":"Int J Food Microbiol"},{"key":"2026102111491985700_bib27","doi-asserted-by":"publisher","first-page":"3468","DOI":"10.1007\/s12010-022-03876-x","article-title":"Antifungal activity of endophytic Aspergillus terreus extract against some fungi causing Mucormycosis: ultrastructural study","volume":"194","author":"Hashem","year":"2022","journal-title":"Appl Biochem Biotechnol"},{"key":"2026102111491985700_bib28","doi-asserted-by":"publisher","first-page":"2411555","DOI":"10.1155\/2023\/2411555","article-title":"Antifungal activity of bioactive compounds produced by the endophytic fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani","volume":"2023","author":"Hawar","year":"2023","journal-title":"Int J Biomater"},{"key":"2026102111491985700_bib29","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s10337-025-04467-8","article-title":"The origins, identification, and solutions for ghost peaks in gas chromatography","volume":"89","author":"Hu","year":"2025","journal-title":"Chromatographia"},{"key":"2026102111491985700_bib30","doi-asserted-by":"publisher","first-page":"15501","DOI":"10.1007\/s11356-021-16640-1","article-title":"Heavy metal tolerant endophytic fungi Aspergillus welwitschiae improves growth, ceasing metal uptake and strengthening antioxidant system in Glycine max L","volume":"29","author":"Husna","year":"2022","journal-title":"Environ Sci Pollut Res"},{"key":"2026102111491985700_bib31","doi-asserted-by":"publisher","first-page":"5","DOI":"10.22092\/botany.2012.101421","article-title":"First report of Chromelosporium fulvum from Iran","volume":"12","author":"Jamali","year":"2015","journal-title":"Rostaniha"},{"key":"2026102111491985700_bib32","doi-asserted-by":"publisher","first-page":"1669","DOI":"10.3390\/antiox11091669","article-title":"Endophytic fungal consortia enhance basal drought-tolerance in Moringa oleifera by upregulating the antioxidant enzyme (APX) through heat shock factors","volume":"11","author":"Javed","year":"2022","journal-title":"Antioxidants"},{"key":"2026102111491985700_bib33","doi-asserted-by":"publisher","first-page":"130","DOI":"10.4014\/JMB.2008.08027","article-title":"Undecanoic acid, lauric acid, and N-tridecanoic acid inhibit Escherichia coli persistence and biofilm formation","volume":"31","author":"Jin","year":"2021","journal-title":"J Microbiol Biotechnol"},{"key":"2026102111491985700_bib34","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/978-3-030-87289-2_4","article-title":"Endophytic microbiome in bioactive compound production and plant disease management","volume-title":"Microbial Biocontrol: Food Security and Post Harvest Management: Volume 2","author":"Kamat","year":"2022"},{"key":"2026102111491985700_bib35","doi-asserted-by":"publisher","first-page":"6351","DOI":"10.1007\/s00018-021-03896-6","article-title":"Primary metabolic processes as drivers of leaf ageing","volume":"78","author":"Kanojia","year":"2021","journal-title":"Cell Mol Life Sci"},{"key":"2026102111491985700_bib36","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol Biol Evol"},{"key":"2026102111491985700_bib37","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1007\/s11033-020-05394-7","article-title":"Antibiofilm, antiproliferative, antioxidant and antimutagenic activities of an endophytic fungus Aspergillus fumigatus from Moringa oleifera","volume":"47","author":"Kaur","year":"2020","journal-title":"Mol Biol Rep"},{"key":"2026102111491985700_bib38","doi-asserted-by":"publisher","first-page":"18792","DOI":"10.1038\/s41598-020-75722-1","article-title":"Bioactive potential of endophytic fungus Chaetomium globosum and GC-MS analysis of its responsible components","volume":"10","author":"Kaur","year":"2020","journal-title":"Sci Rep"},{"key":"2026102111491985700_bib39","doi-asserted-by":"publisher","first-page":"3570","DOI":"10.1007\/s12010-021-03625-6","article-title":"Antiproliferative and oxidative damage protection activities of endophytic fungi Aspergillus fumigatus and Chaetomium globosum from Moringa oleifera Lam","volume":"193","author":"Kaur","year":"2021","journal-title":"Appl Biochem Biotechnol"},{"key":"2026102111491985700_bib40","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.forc.2017.11.002","article-title":"Evaluation of the reproducibility and repeatability of GCMS retention indices and mass spectra of novel psychoactive substances","volume":"7","author":"Kelly","year":"2018","journal-title":"Forensic Chem"},{"key":"2026102111491985700_bib41","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s11557-021-01695-8","article-title":"Biological potential of bioactive metabolites derived from fungal endophytes associated with medicinal plants","volume":"20","author":"Keshri","year":"2021","journal-title":"Mycol Prog"},{"key":"2026102111491985700_bib42","doi-asserted-by":"publisher","first-page":"254","DOI":"10.3390\/jof9020254","article-title":"Diversity, lifestyle, genomics, and their functional role of Cochliobolus, Bipolaris, and Curvularia species in environmental remediation and plant growth promotion under biotic and abiotic stressors","volume":"9","author":"Khan","year":"2023","journal-title":"J Fungi"},{"key":"2026102111491985700_bib43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/agriculture10100450","article-title":"Morphological characterization and determination of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolated from sweet corn kernels and soil in Malaysia","volume":"10","author":"Khan","year":"2020","journal-title":"Agriculture"},{"key":"2026102111491985700_bib44","doi-asserted-by":"publisher","first-page":"9","DOI":"10.3390\/pathogens9010009","article-title":"New species of the genus Curvularia: C. tamilnaduensis and C. coimbatorensis from fungal keratitis cases in South India","volume":"9","author":"Kiss","year":"2020","journal-title":"Pathogens"},{"key":"2026102111491985700_bib45","doi-asserted-by":"publisher","first-page":"1528","DOI":"10.3390\/microorganisms10081528","article-title":"Microorganisms in plant growth and development: roles in abiotic stress tolerance and secondary metabolites secretion","volume":"10","author":"Koza","year":"2022","journal-title":"Microorganisms"},{"key":"2026102111491985700_bib46","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1039\/c3np70127a","article-title":"Secondary metabolomics: the impact of mass spectrometry-based approaches on the discovery and characterization of microbial natural products","volume":"31","author":"Krug","year":"2014","journal-title":"Nat Prod Rep"},{"key":"2026102111491985700_bib47","doi-asserted-by":"publisher","first-page":"4013","DOI":"10.1007\/s11101-024-10028-y","article-title":"Recent advancement in mass based plant metabolomics: techniques, tools, and analytical approaches","volume":"24","author":"Kumar","year":"2025","journal-title":"Phytochem Rev"},{"key":"2026102111491985700_bib48","doi-asserted-by":"publisher","first-page":"463625","DOI":"10.1016\/j.chroma.2022.463625","article-title":"Recent update of the various sources originating ghost peaks in gas chromatography: a review","volume":"1685","author":"Kumar","year":"2022","journal-title":"J Chromatogr A"},{"key":"2026102111491985700_bib49","doi-asserted-by":"publisher","first-page":"1629","DOI":"10.1016\/j.soilbio.2008.01.018","article-title":"Volatile organic compound (VOC) emissions from soil and litter samples","volume":"40","author":"Leff","year":"2008","journal-title":"Soil Biol Biochem"},{"key":"2026102111491985700_bib50","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1016\/j.foreco.2018.09.057","article-title":"Biodiversity response to forest structure and management: comparing species richness, conservation relevant species and functional diversity as metrics in forest conservation","volume":"432","author":"Lelli","year":"2019","journal-title":"Forest Ecol Manag"},{"key":"2026102111491985700_bib51","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1007\/s00284-023-03514-7","article-title":"Current fungal taxonomy and developments in the identification system","volume":"80","author":"Lin","year":"2023","journal-title":"Curr Microbiol"},{"key":"2026102111491985700_bib52","doi-asserted-by":"publisher","first-page":"2561","DOI":"10.1016\/S2095-3119(19)62610-9","article-title":"Evaluation of the biocontrol potential of Aspergillus welwitschiae against the root-knot nematode Meloidogyne graminicola in rice (Oryza sativa L.)","volume":"18","author":"Liu","year":"2019","journal-title":"J Integr Agric"},{"key":"2026102111491985700_bib53","doi-asserted-by":"publisher","first-page":"v","DOI":"10.2144\/000114497","article-title":"Extraction of genomic DNA from yeasts for PCR-based applications","volume":"62","author":"L\u00f5oke","year":"2017","journal-title":"BioTechniques"},{"key":"2026102111491985700_bib54","doi-asserted-by":"publisher","first-page":"6466","DOI":"10.3390\/molecules27196466","article-title":"Recent advances in mass spectrometry-based structural elucidation techniques","volume":"27","author":"Ma","year":"2022","journal-title":"Molecules"},{"key":"2026102111491985700_bib55","doi-asserted-by":"publisher","first-page":"1309","DOI":"10.3390\/molecules26051309","article-title":"Biochemical properties of tyrosinase from Aspergillus terreus and Penicillium copticola; undecanoic acid from Aspergillus flavus, an endophyte of Moringa oleifera, is a novel potent tyrosinase inhibitor","volume":"26","author":"Maamoun","year":"2021","journal-title":"Molecules"},{"key":"2026102111491985700_bib56","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1007\/978-94-015-7358-0_2","article-title":"Diversity indices and species abundance models","volume-title":"Ecological Diversity and Its Measurement","author":"Magurran","year":"1988"},{"key":"2026102111491985700_bib57","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1007\/s00775-020-01802-2","article-title":"Zn(II) and Cd(II) thiosemicarbazones for stimulation\/inhibition of kojic acid biosynthesis from Aspergillus flavus and the fungal defense behavior against the metal complexes\u2019 excesses","volume":"25","author":"Mahmoud","year":"2020","journal-title":"J Biol Inorg Chem"},{"key":"2026102111491985700_bib58","doi-asserted-by":"publisher","first-page":"5263","DOI":"10.1038\/s41598-019-41704-1","article-title":"Morphologic, molecular and metabolic characterization of Aspergillus section Flavi in spices marketed in Lebanon","volume":"9","author":"Makhlouf","year":"2019","journal-title":"Sci Rep"},{"key":"2026102111491985700_bib59","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1007\/s00284-024-04050-8","article-title":"Antimicrobial and cytotoxic potential of endophytic Aspergillus versicolor isolate from the medicinal plant Plectranthus amboinicus","volume":"82","author":"Manaswini","year":"2025","journal-title":"Curr Microbiol"},{"key":"2026102111491985700_bib60","doi-asserted-by":"publisher","first-page":"1590","DOI":"10.3390\/microorganisms12081590","article-title":"Identification, characterization, and antibacterial evaluation of five endophytic fungi from Psychotria poeppigiana M\u00fcll. Arg., an Amazon plant","volume":"12","author":"Mendieta-Brito","year":"2024","journal-title":"Microorganisms"},{"key":"2026102111491985700_bib61","doi-asserted-by":"publisher","first-page":"6927219","DOI":"10.1155\/2020\/6927219","article-title":"Endophytes: colonization, behaviour, and their role in defense mechanism","volume":"2020","author":"Mengistu","year":"2020","journal-title":"Int J Microbiol"},{"key":"2026102111491985700_bib62","doi-asserted-by":"publisher","first-page":"14","DOI":"10.5380\/rsa.v19i1.55844","article-title":"Mycelial growth and sporulation of Fusarium spp. pathogenic to Ilex paraguariensis in different culture media and under exposure to different light levels","volume":"19","author":"Mezzomo","year":"2018","journal-title":"Sci Agrar"},{"key":"2026102111491985700_bib63","doi-asserted-by":"publisher","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol Biol Evol"},{"key":"2026102111491985700_bib64","doi-asserted-by":"publisher","first-page":"63","DOI":"10.3233\/AJW240009","article-title":"Morphological and molecular characterization of endophytic fungi isolated from Moringa oleifera leaves in Iraq and chemical analysis of leaves extracts using GC-MS","volume":"21","author":"Mohammed","year":"2024","journal-title":"Asian J Water Environ Pollut"},{"key":"2026102111491985700_bib65","doi-asserted-by":"publisher","first-page":"3514","DOI":"10.1002\/ece3.1155","article-title":"Choosing and using diversity indices: insights for ecological applications from the German Biodiversity Exploratories","volume":"4","author":"Morris","year":"2014","journal-title":"Ecol Evol"},{"key":"2026102111491985700_bib66","doi-asserted-by":"publisher","first-page":"28","DOI":"10.3390\/jof8010028","article-title":"Recent developments in metabolomics studies of endophytic fungi","volume":"8","author":"Nagarajan","year":"2022","journal-title":"J Fungi"},{"key":"2026102111491985700_bib67","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1007\/s00284-011-0031-1","article-title":"Comparison of bacterial and fungal communities between natural and planted pine forests in subtropical China","volume":"64","author":"Nie","year":"2012","journal-title":"Curr Microbiol"},{"key":"2026102111491985700_bib68","doi-asserted-by":"publisher","first-page":"2113","DOI":"10.3390\/microbiolres14040143","article-title":"Fungal endophytes of Moringa (Moringa oleifera L.), neem (Azadirachta indica) and lavender (Lavandula angustifolia) and their biological control of Fusarium wilt of banana","volume":"14","author":"Nthuku","year":"2023","journal-title":"Microbiol Res"},{"key":"2026102111491985700_bib69","doi-asserted-by":"publisher","first-page":"24","DOI":"10.2991\/978-94-6463-062-6_4","article-title":"Lipase production of Aspergillus aculeatus MS.11 using solid state fermentation on rubber seed press cake","volume-title":"Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)","author":"Nurjanah","year":"2023"},{"key":"2026102111491985700_bib70","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ani10081337","article-title":"A culture-based ID of micromycetes on the wing membranes of greater mouse-eared bats (Myotis myotis) from the \u201cNietoperek\u201d site (Poland)","volume":"10","author":"Og\u00f3rek","year":"2020","journal-title":"Animals"},{"key":"2026102111491985700_bib71","doi-asserted-by":"publisher","first-page":"167","DOI":"10.38150\/sajeb.6(5).p167-177","article-title":"Distribution dynamics of Fusarium spp. causing Fusarium head blight (FHB) in wheat at different geographical locations in India","volume":"6","author":"Panwar","year":"2017","journal-title":"South Asian J Exp Biol"},{"key":"2026102111491985700_bib72","first-page":"180","article-title":"Evaluation of the potency of endophytic fungi extracts associated with potentially medicinal plants from Mandalika-Lombok, West Nusa Tenggara","volume":"10","author":"Praptiwi","year":"2018","journal-title":"J Pharm Sci Res"},{"key":"2026102111491985700_bib73","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.3390\/microorganisms11082025","article-title":"Aspergillus nomiae and fumigatus ameliorating the hypoxic stress induced by waterlogging through ethylene metabolism in Zea mays L","volume-title":"Microorganisms","author":"Rahman","year":"2023"},{"key":"2026102111491985700_bib74","doi-asserted-by":"publisher","first-page":"37","DOI":"10.22159\/ijpps.2016.v8i9.11466","article-title":"Bioactive potential of endophytic fungi Aspergillus flavus (SS03) against clinical isolates","volume":"8","author":"Rajeswari","year":"2016","journal-title":"Int J Pharm Pharm Sci"},{"key":"2026102111491985700_bib75","doi-asserted-by":"publisher","first-page":"66","DOI":"10.14203\/ann.bogor.2020.v24.n2.66-73","article-title":"Study on endophytic fungi associated with Moringa oleifera Lam. collected from Lombok Island, West Nusa Tenggara","volume":"24","author":"Ramadhani","year":"2021","journal-title":"Ann Bogor"},{"key":"2026102111491985700_bib76","doi-asserted-by":"publisher","first-page":"501","DOI":"10.52568\/000751\/jcsp\/41.03.2019","article-title":"Colonization frequency, endophytic infection rate and bioactivities of microbes of desert medicinal plants","volume":"41","author":"Rehman","year":"2019","journal-title":"J Chem Soc Pak"},{"key":"2026102111491985700_bib77","doi-asserted-by":"publisher","first-page":"2461021","DOI":"10.1155\/2022\/2461021","article-title":"Potent bioactivity of endophytic fungi isolated from Moringa oleifera leaves","volume":"2022","author":"Rehman","year":"2022","journal-title":"Biomed Res Int"},{"key":"2026102111491985700_bib78","doi-asserted-by":"publisher","first-page":"29","DOI":"10.4014\/jmb.1208.04062","article-title":"An endophytic Nodulisporium sp. from Central America producing volatile organic compounds with both biological and fuel potential","volume":"23","author":"Riyaz-Ul-Hassan","year":"2013","journal-title":"J Microbiol Biotechnol"},{"key":"2026102111491985700_bib79","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1007\/s11306-018-1361-9","article-title":"Volatile metabolomic signature of bladder cancer cell lines based on gas chromatography\u2013mass spectrometry","volume":"14","author":"Rodrigues","year":"2018","journal-title":"Metabolomics"},{"key":"2026102111491985700_bib80","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-3-031-49112-2_1","article-title":"Endophytic fungi: symbiotic bioresource for production of plant secondary metabolites","author":"Saini","year":"2024"},{"key":"2026102111491985700_bib81","doi-asserted-by":"publisher","first-page":"147","DOI":"10.3114\/sim.2007.59.14","article-title":"Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya","volume":"59","author":"Samson","year":"2007","journal-title":"Stud Mycol"},{"key":"2026102111491985700_bib82","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1007\/s00248-015-0679-3","article-title":"Antifungal volatile organic compounds from the endophyte Nodulisporium sp. strain GS4d2II1a: a qualitative change in the intraspecific and interspecific interactions with Pythium aphanidermatum","volume":"71","author":"S\u00e1nchez-Fern\u00e1ndez","year":"2016","journal-title":"Microb Ecol"},{"key":"2026102111491985700_bib83","doi-asserted-by":"publisher","first-page":"2678","DOI":"10.5943\/Mycosphere\/11\/1\/20","article-title":"Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation","volume":"11","author":"Senanayake","year":"2020","journal-title":"Mycosphere"},{"key":"2026102111491985700_bib84","first-page":"981","article-title":"Histone deacetylase inhibitory activity and antiproliferative activity of the cultured medium of Aspergillus niger strain TS1","volume":"41","author":"Senawong","year":"2014","journal-title":"Chiang Mai J Sci"},{"key":"2026102111491985700_bib85","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/B978-0-323-99314-2.00018-8","article-title":"Secondary metabolites production by endophytic fungi","volume-title":"Endophytic Fungi: The Full Story of the Untapped Treasure","author":"Serag","year":"2024"},{"key":"2026102111491985700_bib86","doi-asserted-by":"publisher","first-page":"4141480","DOI":"10.1155\/2022\/4141480","article-title":"Fusarium oxysporum & Fusarium solani: identification, characterization, and differentiation of the fungal phenolic profiles by HPLC and the fungal lipid profiles by GC-MS","volume":"2022","author":"Shalapy","year":"2022","journal-title":"J Food Qual"},{"key":"2026102111491985700_bib87","doi-asserted-by":"publisher","first-page":"11976","DOI":"10.1038\/s41598-020-68706-8","article-title":"FKS1 mutation associated with decreased echinocandin susceptibility of Aspergillus fumigatus following anidulafungin exposure","volume":"10","author":"e\u00a0Silva","year":"2020","journal-title":"Sci Rep"},{"key":"2026102111491985700_bib88","doi-asserted-by":"publisher","first-page":"1","DOI":"10.9734\/bmrj\/2016\/24722","article-title":"Endophytic microorganisms in leaves of Moringa oleifera collected in three localities at Pernambuco State, northeastern Brazil","volume":"13","author":"Souza","year":"2016","journal-title":"Br Microbiol Res J"},{"key":"2026102111491985700_bib89","doi-asserted-by":"publisher","first-page":"351","DOI":"10.14199\/ppp-2020-039","article-title":"Chromelosporium fulvum (Link) McGinty, Hennebert & Korf\u2014a new danger for houseplants","volume":"60","author":"St\u0119pniewska-Jarosz","year":"2020","journal-title":"Prog Plant Prot"},{"key":"2026102111491985700_bib90","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1080\/00275514.2018.1432221","article-title":"Multilocus phylogenetic analysis of Talaromyces species isolated from cucurbit plants in China and description of two new species, T. cucurbitiradicus and T. endophyticus","volume":"110","author":"Su","year":"2018","journal-title":"Mycologia"},{"key":"2026102111491985700_bib91","doi-asserted-by":"publisher","first-page":"7899","DOI":"10.26538\/tjnpr\/v8i7.37","article-title":"The comparison of phytocompounds and antibacterial activity of Moringa oleifera leaves and its endophytic fungi on different environment conditions","volume":"8","author":"Sukmawaty","year":"2024","journal-title":"Trop J Nat Prod Res"},{"key":"2026102111491985700_bib92","doi-asserted-by":"publisher","first-page":"799","DOI":"10.21162\/pakjas\/17.5517","article-title":"Production and characterization of phytase from indigenous Aspergillus niger isolates","volume":"54","author":"Tariq","year":"2017","journal-title":"Pak J Agric Sci"},{"key":"2026102111491985700_bib93","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1127\/nova_hedwigia\/2019\/0528","article-title":"Utility of various molecular markers in fungal identification and phylogeny","volume":"109","author":"Tekpinar","year":"2019","journal-title":"Nova Hedwigia"},{"key":"2026102111491985700_bib94","doi-asserted-by":"publisher","first-page":"80","DOI":"10.3390\/agriculture7100080","article-title":"Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya","volume":"7","author":"Thathana","year":"2017","journal-title":"Agriculture"},{"key":"2026102111491985700_bib95","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00488-17","article-title":"Novel ITS1 fungal primers for characterization of the mycobiome","volume-title":"mSphere","author":"Usyk","year":"2017"},{"key":"2026102111491985700_bib96","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/j.ejbas.2016.08.007","article-title":"Diversity and extracellular enzyme activities of fungal endophytes isolated from medicinal plants of Western Ghats, Karnataka","volume":"3","author":"Uzma","year":"2016","journal-title":"Egypt J Basic Appl Sci"},{"key":"2026102111491985700_bib97","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1186\/s12866-018-1244-2","article-title":"Comparative genotyping and phenotyping of Aspergillus fumigatus isolates from humans, dogs and the environment","volume":"18","author":"Valdes","year":"2018","journal-title":"BMC Microbiol"},{"key":"2026102111491985700_bib98","doi-asserted-by":"publisher","first-page":"3297","DOI":"10.1039\/d0cs00162g","article-title":"Linking genomics and metabolomics to chart specialized metabolic diversity","volume":"49","author":"van\u00a0der\u00a0Hooft","year":"2020","journal-title":"Chem Soc Rev"},{"key":"2026102111491985700_bib99","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1039\/d3np00050h","article-title":"Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products","volume":"41","author":"Vitale","year":"2024","journal-title":"Nat Prod Rep"},{"key":"2026102111491985700_bib100","doi-asserted-by":"publisher","first-page":"45","DOI":"10.3390\/metabo7030045","article-title":"Recommendations for improving identification and quantification in non-targeted, GC-MS-based metabolomic profiling of human plasma","volume":"7","author":"Wang","year":"2017","journal-title":"Metabolites"},{"key":"2026102111491985700_bib101","doi-asserted-by":"publisher","first-page":"997","DOI":"10.3390\/jof9100997","article-title":"Health-promoting effects of bioactive compounds from plant endophytic fungi","volume":"9","author":"Wijesekara","year":"2023","journal-title":"J Fungi"},{"key":"2026102111491985700_bib102","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.jbiosc.2018.02.001","article-title":"Isolation and identification of Penicillium chrysogenum strain Y5 and its copper extraction characterization from waste printed circuit boards","volume":"126","author":"Xia","year":"2018","journal-title":"J Biosci Bioeng"},{"key":"2026102111491985700_bib103","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1111\/lam.12483","article-title":"Diversity and antimicrobial activity of endophytic fungi isolated from Cephalotaxus hainanensis Li, a well-known medicinal plant in China","volume":"61","author":"Yang","year":"2015","journal-title":"Lett Appl Microbiol"},{"key":"2026102111491985700_bib104","doi-asserted-by":"publisher","first-page":"2351","DOI":"10.1007\/s11274-011-0702-x","article-title":"The diversity of arbuscular mycorrhizal fungi in the subtropical forest of Huangshan (Yellow Mountain), East-Central China","volume":"27","author":"Yang","year":"2011","journal-title":"World J Microbiol Biotechnol"},{"key":"2026102111491985700_bib105","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.simyco.2014.08.001","article-title":"Polyphasic taxonomy of the genus Talaromyces","volume":"78","author":"Yilmaz","year":"2014","journal-title":"Stud Mycol"},{"key":"2026102111491985700_bib106","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1080\/12298093.2018.1454008","article-title":"Diversity and antifungal activity of endophytic fungi associated with Camellia oleifera","volume":"46","author":"Yu","year":"2018","journal-title":"Mycobiology"},{"key":"2026102111491985700_bib107","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/B978-012509551-8\/50008-8","article-title":"Fungal biodiversity patterns","volume-title":"Biodiversity of Fungi: Inventory and Monitoring Methods","author":"Zak","year":"2004"},{"key":"2026102111491985700_bib108","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1186\/s42269-024-01199-x","article-title":"Progress in endophytic fungi secondary metabolites: biosynthetic gene cluster reactivation and advances in metabolomics","volume":"48","author":"Zakariyah","year":"2024","journal-title":"Bull Natl Res Cent"},{"key":"2026102111491985700_bib109","doi-asserted-by":"publisher","first-page":"117068","DOI":"10.1016\/j.jpba.2025.117068","article-title":"Optimization of GC-MS run time for untargeted metabolomics: trade-offs between speed, coverage, and repeatability","volume":"266","author":"Zeki","year":"2025","journal-title":"J Pharm Biomed Anal"},{"key":"2026102111491985700_bib110","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1111\/eva.12694","article-title":"Metabarcoding using multiplexed markers increases species detection in complex zooplankton communities","volume":"11","author":"Zhang","year":"2018","journal-title":"Evol Appl"},{"key":"2026102111491985700_bib111","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fnut.2022.873657","article-title":"Production, biosynthesis, and commercial applications of fatty acids from oleaginous fungi","volume":"9","author":"Zhang","year":"2022","journal-title":"Front Nutr"},{"key":"2026102111491985700_bib112","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1111\/j.1472-765X.2010.02867.x","article-title":"A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains","volume":"51","author":"Zhang","year":"2010","journal-title":"Lett Appl Microbiol"},{"key":"2026102111491985700_bib113","doi-asserted-by":"publisher","first-page":"e0211613","DOI":"10.1371\/journal.pone.0211613","article-title":"Bioconversion of Fructus sophorae into 5,7,8,4\u2019-tetrahydroxyisoflavone with Aspergillus aculeatus","volume":"14","author":"Zhang","year":"2019","journal-title":"PLoS One"}],"container-title":["Journal of Applied Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jambio\/advance-article-pdf\/doi\/10.1093\/jambio\/lxag249\/71626535\/lxag249.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/10\/lxag249\/71626535\/lxag249.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jambio\/article-pdf\/137\/10\/lxag249\/71626535\/lxag249.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:49:28Z","timestamp":1792597768000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jambio\/article\/doi\/10.1093\/jambio\/lxag249\/8868917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":113,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jambio\/lxag249","relation":{},"ISSN":["1365-2672"],"issn-type":[{"value":"1365-2672","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,10]]},"published":{"date-parts":[[2026,10,1]]},"article-number":"lxag249"}}