{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:29:50Z","timestamp":1788571790378,"version":"build-2803163510"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T00:00:00Z","timestamp":1602806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Graduate School of Khon Kaen University","award":["611CH112"],"award-info":[{"award-number":["611CH112"]}]},{"name":"Graduate Development Scholarship 2020"},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"name":"International Research Network for Functional Food Discovery and Development"},{"name":"FFDD"},{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"crossref","award":["IRN61W0005"],"award-info":[{"award-number":["IRN61W0005"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Quassinoids and canthin-6-one alkaloids are bioactive markers of Eurycoma longifolia (EL) and E. harmandiana (EH) and have been commercially utilized to treat inflammation and male infertility.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study aims to reveal the contents of bioactive compounds and compare anti-inflammatory activities of these two species.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>HPLC methods coupled with UV-Vis detection were developed and validated for the simultaneous analysis of the chemical profiles and their contents in EL and EH. The anti-inflammatory activities of both species were investigated using RAW 264.7 cell line.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The HPLC methods provided a sensitivity (LOD) of 0.02\u20130.05\u2009\u00b5g\/mL for the eight bioactive compounds (canthin-6-one alkaloids, quassinoids, and scopoletin) with high precision (% relative standard deviation (RSD) \u22646.48) and recoveries between 80.0 and 120%. The chaparrinone: eurycomanone ratio was high in EH, whereas EL had a higher ratio of eurycomanone: chaparrinone than EH. The contents of total canthin-6-one alkaloids, quassinoids, and scopoletin were 0.01\u20130.75, 0.19\u20131.54, and 0.01\u20130.28\u2009mg\/g, respectively, in EL roots and 0.12\u20131.80, 7.05\u20139.26, and 0.02\u2009mg\/g, respectively, in EH roots. The anti-inflammatory effects of EL and EH extracts varied among the samples due to the variation in their chemical constituents.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In summary, our study indicated that chaparrinone was the major compound in EH. EH exhibited anti-inflammatory activity to the same extent as EL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:p>EH and EL extracts were analyzed using developed HPLC-UV methods, revealing a high concentration of chaparrinone in EH, and an anti-inflammatory assay indicated that EH had a potency comparable to that of EL.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsaa141","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T11:22:40Z","timestamp":1601983360000},"page":"802-810","source":"Crossref","is-referenced-by-count":16,"title":["HPLC-UV-Based Simultaneous Determination of Canthin-6-One Alkaloids, Quassinoids, and Scopoletin: The Active Ingredients in <i>Eurycoma Longifolia<\/i> Jack and <i>Eurycoma Harmandiana<\/i> Pierre, and Their Anti-Inflammatory Activities"],"prefix":"10.1093","volume":"104","author":[{"given":"Jiranan","family":"Chaingam","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaweesak","family":"Juengwatanatrakul","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorawit","family":"Yusakul","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Walailak University, 222 Thaiburi, Thasala, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tripetch","family":"Kanchanapoom","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waraporn","family":"Putalun","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,16]]},"reference":[{"key":"2021071316410852900_qsaa141-B1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1016\/j.jep.2012.11.013","volume":"145","author":"Low","year":"2013","journal-title":"J. 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