{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:20Z","timestamp":1787009060786,"version":"build-2736575974"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Academy of National Food and Strategic Reserves Administration of People\u2019s Republic of China"},{"name":"Grain Industry Standard of China","award":["HB1804"],"award-info":[{"award-number":["HB1804"]}]},{"name":"Special Survey of Basic Scientific and Technological Resources","award":["2022FY101202"],"award-info":[{"award-number":["2022FY101202"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFF1101103"],"award-info":[{"award-number":["2022YFF1101103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>As the unique ingredient of cottonseed oil, gossypol is toxic and there are differences between the enantiomers. Although the determination of (-)- and (+)-gossypol in cotton plants or cottonseed has been performed, the detection of the gossypol enantiomers in vegetable oil has rarely been reported.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To develop a specific inspection method for (-)- and (+)-gossypol in vegetable oil by ultra-performance liquid chromatography (UPLC)\u2013tandem mass spectrometry with chiral pre-column derivatization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>(-)- and (+)-Gossypol were separated on an ACQUITY C18 reverse-phase column (100\u2009mm \u00d7 2.1\u2009mm, 1.7\u2009\u03bcm) maintained at 40\u00b0C on an UPLC system with gradient elution. The mobile phases were composed of methanol (solvent A) and water containing 0.1% formic acid (solvent B). Analytes were detected using an electrospray ionization (ESI) source in positive mode. The contents of (-)- and (+)-gossypol standard derivatives were quantified by multiple reaction monitoring (MRM) with the transitions of m\/z 633\/483 for quantitative ion pairs and m\/z 633\/558 for qualitative ion pairs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The results indicated that the quantitative analysis could be accomplished within 12.5\u2009min and the LODs of (-)- and (+)-gossypol were 25.55 and 14.67\u2009\u03bcg\/kg, respectively. When this method was applied to olive oil spiked with gossypol standards, good recoveries [95.37\u2013105.84% for (-)-gossypol and 98.96\u2013105.14% for (+)-gossypol] and RSDs [3.41\u20136.02% for (-)-gossypol and 4.50\u20136.94% for (+)-gossypol)] were achieved.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study confirms the feasibility of determining gossypol enantiomers and achieves trace determination of (-)- and (+)-gossypol in vegetable oil. These results make this method more suitable for the qualitative identification of whether the vegetable oil has been mixed with cottonseed oil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>The trace determination of (-)- and (+)-gossypol in vegetable oils has been achieved. This method provides technical support for further identification of whether commercially available vegetable oil has been mixed with cottonseed oil.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsae076","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T23:22:31Z","timestamp":1728343351000},"page":"144-150","source":"Crossref","is-referenced-by-count":3,"title":["Specific Determination of (-)- and (+)-Gossypol in Vegetable Oil by Ultra-Performance Liquid Chromatography\u2013Tandem Mass Spectrometry with Chiral Pre-Column Derivatization"],"prefix":"10.1093","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4749-9332","authenticated-orcid":false,"given":"Mimi","family":"Guo","sequence":"first","affiliation":[{"name":"Institute of Cereal & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration , Beijing 102209,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1064-9060","authenticated-orcid":false,"given":"Yameng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Bejing Pharmaceutical University of Staff and Workers , Beijing 100079,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5235-9318","authenticated-orcid":false,"given":"Yalin","family":"Xue","sequence":"additional","affiliation":[{"name":"Institute of Cereal & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration , Beijing 102209,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-2798","authenticated-orcid":false,"given":"Zhangqun","family":"Duan","sequence":"additional","affiliation":[{"name":"Institute of Cereal & Oil Science and Technology, Academy of National Food and Strategic Reserves Administration , Beijing 102209,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6995-8386","authenticated-orcid":false,"given":"Qiaoying","family":"Chang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Inspection and Quarantine , Beijing 100176,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"2025031309272507200_qsae076-B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/07352689.2022.2156061","volume":"42","author":"Viot","year":"2023","journal-title":"Crit. 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