{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T23:10:04Z","timestamp":1746227404475,"version":"3.40.4"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Hormones regulate growth, development, and reproductive functions. While deficiencies can lead to various health problems, excessive intake may cause metabolic disorders and side effects. Therefore, there is a need to develop new, reliable, and economical methods for QC and accurate analysis of hormones in tablets used for hormone replacement therapy (HRT).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To develop a novel, reliable, and cost-effective, ultra-performance liquid chromatography (UPLC) method for accurately quantifying estradiol valerate (EV) and medroxyprogesterone acetate (MPG) in HRT tablets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Chromatographic separation of EV and MPG was achieved using an ACQUITY UPLC BEH C18 (1.7\u2009\u03bcm, 2.1\u2009\u00d7\u2009100\u2009mm) column and a mixture containing water\u2013acetonitrile\u2013methanol\u20130.10 M phosphate buffer pH 2.14 (6:44:42:8, v\/v). Chromatographic detection was accomplished at 280.0\u2009nm for EV and 240.0\u2009nm for MPG, with a flow rate of 0.34\u2009mL\/min at a column temperature of 40\u00b0C. The method's validity was assessed using individual and spiked sample analyses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The validated UPLC method offered an alternative procedure to quantify EV and MPG in commercial hormone tablets. This method demonstrated highly accurate and precise analytic results with a short retention time and low cost. The measured concentrations of EV and MPG in the tablet samples were found within the expected ranges, confirming the method's suitability for reliable hormone quantification in commercial tablets.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This validated UPLC method is a cost-effective and efficient tool for routine quality control of EV and MPG in HRT tablets, ensuring reliable pharmaceutical analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:p>This study introduces the first ultra-performance liquid chromatography with photodiode array detection (UPLC\u2013PDA) method for analyzing EV and MPG in a commercial hormone formulation. The method showed excellent assay results in the analysis of real samples.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsaf016","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T21:50:17Z","timestamp":1741125017000},"page":"313-319","source":"Crossref","is-referenced-by-count":0,"title":["Co-Estimating Estradiol Valerate and Medroxyprogesterone Acetate in a Combined Hormonal Product: A Novel Short Runtime UPLC Method"],"prefix":"10.1093","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6326-1441","authenticated-orcid":false,"given":"Erdal","family":"Din\u00e7","sequence":"first","affiliation":[{"name":"Ankara University, Department of Analytical Chemistry, Faculty of Pharmacy , 06560 Yenimahalle, Ankara,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8289-2238","authenticated-orcid":false,"given":"Adem","family":"Mert","sequence":"additional","affiliation":[{"name":"Ankara University, Department of Analytical Chemistry, Faculty of Pharmacy , 06560 Yenimahalle, Ankara,","place":["Turkey"]},{"name":"Ankara University, Department of Analytical Chemistry, Graduate School of Health Sciences , 06110 D\u0131\u015fkap\u0131, Ankara,","place":["Turkey"]},{"name":"Gaziantep University, Department of Veterinary, Araban Vocational School , 27650 Araban, Gaziantep,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"2025050218521604100_qsaf016-B1","doi-asserted-by":"publisher","first-page":"124159","DOI":"10.1016\/j.jchromb.2024.124159","volume":"1240","author":"\u00dcst\u00fcnda\u011f","year":"2024","journal-title":"J. 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