{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:41:37Z","timestamp":1792644097714,"version":"4.5.0"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n D-Glucose and D-fructose are present in honey, wine and beer, and in a range of other foodstuffs such as bread, pastries, or chocolate. Both sugars can occur as a monosaccharide or in di-, oligo-, and polysaccharides.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To validate the performance of the Enzytec\u2122 Liquid D-Glucose\/D-Fructose test kit for the determination of D-glucose and D-fructose in food and beverages such as fruit and vegetable juices, soft drinks, wines, and beer.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n The method is based on enzymes that are part of a prepackaged kit that contains three ready-to-use components. Both sugars are phosphorylated by a hexokinase. Glucose-6-phosphate (G-6-P) and nicotinamide adenine dinucleotide (NAD) are converted to gluconate-6-phosphate and reduced nicotinamide adenine dinucleotide (NADH). NADH is measured at 340\u2009nm. Phosphoglucose isomerase converts fructose-6-phosphate to G-6-P, which in turn is converted to gluconate-6-phosphate and NADH.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The test is specific to D-glucose and D-fructose and shows no side-activities or interferences with the exception of mannose and sulphite that interfere at 5.1 and 1.25\u2009g\/L or more, respectively. The measurement range is from 6.1 to 2000\u2009mg\/L for D-glucose and 5.6 to 1000\u2009mg\/L for D-fructose (100\u2009\u00b5L test volume). Trueness was evaluated using NIST SRM 3282 (cranberry juice) and one reference wine. The recoveries ranged from 101 to 102%. Spiking of wine, beer, soft drinks, and juices resulted in recoveries between 93 and 105%. Intermediate precision is below 6% for concentrations at 25\u2009mg\/L and below 4% for higher concentrations. For automation, three applications with different test volumes and different measurement ranges were validated. Linearity is given from 5.0 to 10\u200a000\u2009mg\/L for the sum of both sugars.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The method is robust and accurate for manual and automated applications and was approved as an AOAC Official Method of Analysis\u2120.<\/jats:p>\n <\/jats:sec>\n \n Highlights<\/jats:title>\n The ready-to-use components of the test kit have a shelf life of at least 29\u2009months.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsaf019","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T09:02:10Z","timestamp":1741251730000},"page":"572-594","source":"Crossref","is-referenced-by-count":5,"title":["Validation of Enzytec\u2122\n Liquid<\/i>\n D-Glucose\/D-Fructose for Enzymatic Determination of D-Glucose and D-Fructose in Selected Foods and Beverages: Official Method 2024.04 First Action"],"prefix":"10.1093","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9455-1249","authenticated-orcid":false,"given":"Markus","family":"Lacorn","sequence":"first","affiliation":[{"name":"R&D Food&Feed, R-Biopharm AG , An der Neuen Bergstr. 17 , 64297 Darmstadt,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4545-4597","authenticated-orcid":false,"given":"Thomas","family":"Hektor","sequence":"additional","affiliation":[{"name":"R&D Food&Feed, R-Biopharm AG , An der Neuen Bergstr. 17 , 64297 Darmstadt,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"2025070712465734500_qsaf019-B1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/B978-0-12-384947-2.00353-6","volume-title":"The Encyclopedia of Food and Health","author":"Shendurse","year":"2016"},{"key":"2025070712465734500_qsaf019-B2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-30429-6_2","volume-title":"Glycoscience","author":"Robyt","year":"2008"},{"key":"2025070712465734500_qsaf019-B3","first-page":"9","volume":"223","author":"Wilkes","year":"2016","journal-title":"AWRI Tech. Rev"},{"key":"2025070712465734500_qsaf019-B4","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.5740\/jaoacint.SMPR2018.001","volume":"101","author":"Szpylka","year":"2018","journal-title":"J. AOAC Int"},{"key":"2025070712465734500_qsaf019-B5","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.5740\/jaoacint.17-0466","volume":"101","author":"Lacorn","year":"2018","journal-title":"J. AOAC Int"},{"key":"2025070712465734500_qsaf019-B6","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1093\/jaoacint\/qsac145","volume":"106","author":"Lacorn","year":"2023","journal-title":"J. AOAC Int"},{"key":"2025070712465734500_qsaf019-B7","author":"Linsinger"}],"container-title":["Journal of AOAC International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jaoac\/advance-article-pdf\/doi\/10.1093\/jaoacint\/qsaf019\/62375483\/qsaf019.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/108\/4\/572\/62375483\/qsaf019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/108\/4\/572\/62375483\/qsaf019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T12:47:04Z","timestamp":1751892424000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/108\/4\/572\/8069458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,11]]},"references-count":7,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,3,11]]},"published-print":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jaoacint\/qsaf019","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"value":"1060-3271","type":"print"},{"value":"1944-7922","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,7]]},"published":{"date-parts":[[2025,3,11]]}}}