{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T20:25:43Z","timestamp":1762028743194,"version":"build-2065373602"},"reference-count":5,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Lactose is a disaccharide composed of D-galactose and D-glucose. Lactose composes 2\u20138% of milk by weight. Lactose is digested in the small intestine by the enzyme lactase. Lactase deficiency can lead to lactose intolerance in adults.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n Enzytec\u2122 Liquid Combi Lactose\/D-Galactose is an enzymatic test kit for the determination of lactose and D-galactose in milk, milk products, infant formula, chocolate, sausage meat, almond milk, salad dressing, bread, and cookies. Excluded are lactose-free dairy products that have been enzymatically treated with lactase.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n The kit contains two sets of reagents in a ready-to-use format and is suitable for automation. Lactose is cleaved with \u00df-galactosidase. Resulting D-galactose is oxidized by galactose-dehydrogenase and NAD+. The amount of resulting NADH is equivalent to the converted amount of lactose and free D-galactose. For measurement of free D-galactose alone the second set of reagents is used.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Trueness of the lactose measurement was checked using certified reference materials with recoveries for milk, milk products, infant formula, and chocolate between 94 and 103%. Spiking experiments at low levels in almond milk, salad dressing, feta cheese, cookies, and bread revealed recoveries between 92 and 121%. Intermediate precision varied between 3 and 10.6% for lactose and between 6.5 and 7.8% for D-galactose. For automation, three applications with different test volumes were validated. Linearity is given from 12.5\u2009mg\/L to 12.5\u2009g\/L for lactose and 4.0\u2009mg\/L up to 6.25\u2009g\/L for galactose.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n The method is robust and accurate for manual and automated applications. The method was approved as AOAC Official Method of AnalysisSM.<\/jats:p>\n <\/jats:sec>\n \n Highlights<\/jats:title>\n The ready-to-use components of the test kit have a shelf life of at least 24\u2009months.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsaf070","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T11:54:12Z","timestamp":1753271652000},"page":"901-925","source":"Crossref","is-referenced-by-count":0,"title":["Validation of Enzytec\u2122\n Liquid<\/i>\n Combi Lactose\/D-Galactose for Enzymatic Determination of Lactose and D-Galactose in Selected Foods: Official Method 2024.10 First Action"],"prefix":"10.1093","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9455-1249","authenticated-orcid":false,"given":"Markus","family":"Lacorn","sequence":"first","affiliation":[{"name":"R&D Food&Feed, R-Biopharm AG , An der Neuen Bergstr. 17 , 64297 Darmstadt,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4545-4597","authenticated-orcid":false,"given":"Thomas","family":"Hektor","sequence":"additional","affiliation":[{"name":"R&D Food&Feed, R-Biopharm AG , An der Neuen Bergstr. 17 , 64297 Darmstadt,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"2025110116203974100_qsaf070-B1","doi-asserted-by":"publisher","first-page":"2553","DOI":"10.3390\/foods12132553","volume":"12","author":"Li","year":"2023","journal-title":"Foods"},{"volume-title":"Food Composition and Nutrition Tables","year":"2016","author":"Souci","key":"2025110116203974100_qsaf070-B2"},{"key":"2025110116203974100_qsaf070-B3","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.5740\/jaoacint.17-0466","volume":"101","author":"Lacorn","year":"2018","journal-title":"J. AOAC Int"},{"key":"2025110116203974100_qsaf070-B4","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/S0308-8146(03)00268-1","volume":"84","author":"Marconi","year":"2004","journal-title":"Food Chemistry"},{"key":"2025110116203974100_qsaf070-B5","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.1093\/jaoacint\/qsab032","volume":"104","author":"Ivory","year":"2021","journal-title":"J. AOAC Int"}],"container-title":["Journal of AOAC International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jaoac\/advance-article-pdf\/doi\/10.1093\/jaoacint\/qsaf070\/63841743\/qsaf070.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/108\/6\/901\/63841743\/qsaf070.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/108\/6\/901\/63841743\/qsaf070.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T20:20:50Z","timestamp":1762028450000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/108\/6\/901\/8211917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,24]]},"references-count":5,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,7,24]]},"published-print":{"date-parts":[[2025,11,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jaoacint\/qsaf070","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"type":"print","value":"1060-3271"},{"type":"electronic","value":"1944-7922"}],"subject":[],"published-other":{"date-parts":[[2025,11]]},"published":{"date-parts":[[2025,7,24]]}}}