{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T20:13:37Z","timestamp":1788984817270,"version":"build-2803163510"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T00:00:00Z","timestamp":1772150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"AMBROSIA"},{"name":"Department of Agriculture Food and the Marine"},{"name":"European Joint Programming Initiative"},{"name":"A Healthy Diet for a Healthy Life"},{"name":"ERA-NET Co"},{"name":"ERA HDHL","award":["696295"],"award-info":[{"award-number":["696295"]}]},{"name":"EU Horizon 2020 Research and Innovation Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pesticide residue analysis is a critical component of food safety monitoring. While multiresidue methods based on LC\u2013 or GC\u2013MS are widely available, the analysis of highly polar or ionic pesticides remains challenging due to poor chromatographic retention, low recoveries, and repeatability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to develop a simple, rapid, and robust analytical method for the determination of highly polar or ionic pesticides and their metabolites in selected agricultural commodities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The method targets herbicides, including glyphosate, aminomethylphosphonic acid, glufosinate, 3-methylphosphinicopropionic acid, and N-acetyl-glufosinate, the plant growth regulator ethephon, and a fungicide, fosetyl-Al, and its metabolite phosphonic acid. Sample preparation involved acidified methanolic extraction without cleanup or derivatization. Analysis was performed using LC coupled with tandem mass spectrometry (MS\/MS). Chromatographic conditions were optimized to achieve baseline separation of all target analytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method demonstrated satisfactory performance across fruit matrixes including apple, orange, tomato, cucumber, watermelon, and strawberry. Recoveries ranged from 79 to 103%, with precision values below 13% at all levels tested. The method achieved low matrix effects and met the maximum residue levels established for the target analytes in the respective commodities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The developed method provides a reliable and efficient approach for the analysis of highly polar pesticides and their metabolites in agricultural commodities, addressing limitations associated with conventional multiresidue methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>An analytical method was developed for the determination of highly polar and ionic pesticides that are traditionally challenging to analyze. The method employs a simple sample preparation approach, enabling higher analytical throughput. Validation results demonstrated satisfactory performance as per the the analytical quality control requirements outlined in the SANTE\/11312\/2021 (V2) guidelines. The method complies with national (FSSAI) as well as international (EU) maximum residue limits.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsag016","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:53:27Z","timestamp":1773924807000},"page":"688-694","source":"Crossref","is-referenced-by-count":0,"title":["Analytical Method for Detecting Herbicides, Plant Growth Regulators, and Fungicides in Agricultural Commodities: Single-Laboratory Validation"],"prefix":"10.1093","volume":"109","author":[{"given":"Manisha","family":"Dhanshetty","sequence":"first","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin , Belfield , Dublin D04 V1W8,","place":["Ireland"]},{"name":"UG & PG Department of Microbiology, Yashwantrao Mohite College, Bharati Vidyapeeth (Deemed to be) University, Erandwane , Pune, Maharashtra 411 038,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Garcia-Vaquero","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Science, University College Dublin , Belfield , Dublin D04 V1W8,","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5928-4042","authenticated-orcid":false,"given":"Raviraj Chandrakant","family":"Shinde","sequence":"additional","affiliation":[{"name":"Department of Agrochemicals and Pest Management, Devchand College (Affiliated to Shivaji University, Kolhapur), Nippani, Maharashtra 400615,","place":["India"]},{"name":"Global R&D Laboratory, PerkinElmer , Ghodbunder Road, Kasaravadavali , Thane, Maharashtra 400615,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2437-3787","authenticated-orcid":false,"given":"Gajanan","family":"Mali","sequence":"additional","affiliation":[{"name":"UG & PG Department of Microbiology, Yashwantrao Mohite College, Bharati Vidyapeeth (Deemed to be) University, Erandwane , Pune, Maharashtra 411 038,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pandit","family":"Shiragave","sequence":"additional","affiliation":[{"name":"Department of Agrochemicals and Pest Management, Devchand College (Affiliated to Shivaji University, Kolhapur), Nippani, Maharashtra 400615,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sagar","family":"Atugade","sequence":"additional","affiliation":[{"name":"Global R&D Laboratory, PerkinElmer , Ghodbunder Road, Kasaravadavali , Thane, Maharashtra 400615,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,2,27]]},"reference":[{"key":"2026090915261870000_qsag016-B1","volume-title":"Plant Growth Regulators in Agriculture and Horticulture: Their Role and Commercial Uses","author":"Basra","year":"2000"},{"key":"2026090915261870000_qsag016-B2","volume-title":"The Pesticide Manual: A World Compendium","author":"Tomlin","year":"2009"},{"key":"2026090915261870000_qsag016-B3","doi-asserted-by":"publisher","first-page":"826","DOI":"10.3390\/ijerph14070826","volume":"14","author":"Li","year":"2017","journal-title":"IJERPH"},{"key":"2026090915261870000_qsag016-B4","doi-asserted-by":"publisher","DOI":"10.4060\/cb4769en","volume-title":"Scientific Review of the Impact of Climate Change on Plant Pests: A Global Challenge to Prevent and Mitigate Plant-Pest Risks in Agriculture, Forestry and Ecosystems","author":"Gullino","year":"2021"},{"key":"2026090915261870000_qsag016-B5","author":"EU-MRL Pesticides Database","year":"2025"},{"key":"2026090915261870000_qsag016-B6","author":"Food Safety and Standards Authority of India (FSSAI)","year":"2025"},{"key":"2026090915261870000_qsag016-B7","first-page":"527","volume":"3","author":"Ye","year":"2018","journal-title":"Food Beverage"},{"key":"2026090915261870000_qsag016-B8","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1093\/jaoac\/86.2.412","volume":"86","author":"Anastassiades","year":"2003","journal-title":"J. AOAC Int"},{"key":"2026090915261870000_qsag016-B9","doi-asserted-by":"publisher","first-page":"461416","DOI":"10.1016\/j.chroma.2020.461416","volume":"1627","author":"Kumar","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"2026090915261870000_qsag016-B10","doi-asserted-by":"publisher","first-page":"462346","DOI":"10.1016\/j.chroma.2021.462346","volume":"1652","author":"Shinde","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"2026090915261870000_qsag016-B11","doi-asserted-by":"publisher","first-page":"1528","DOI":"10.1093\/jaoacint\/qsaa066","volume":"103","author":"Shinde","year":"2020","journal-title":"J. AOAC Int"},{"key":"2026090915261870000_qsag016-B12","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.1093\/jaoacint\/qsac019","volume":"105","author":"Shinde","year":"2022","journal-title":"J. AOAC Int"},{"key":"2026090915261870000_qsag016-B13","doi-asserted-by":"publisher","first-page":"3073","DOI":"10.1007\/s00216-024-05257-1","volume":"416","author":"Liu","year":"2024","journal-title":"Anal. Bioanal. Chem"},{"key":"2026090915261870000_qsag016-B14","doi-asserted-by":"publisher","first-page":"38","DOI":"10.3390\/mps7030038","volume":"7","author":"Masci","year":"2024","journal-title":"MPs"},{"key":"2026090915261870000_qsag016-B15","doi-asserted-by":"publisher","first-page":"631","DOI":"10.5740\/jaoacint.17-0046","volume":"100","author":"Oulkar","year":"2017","journal-title":"J. AOAC Int"},{"key":"2026090915261870000_qsag016-B16","doi-asserted-by":"publisher","first-page":"461603","DOI":"10.1016\/j.chroma.2020.461603","volume":"1632","author":"Dovidauskas","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"2026090915261870000_qsag016-B17","author":"Anastassiades","year":"2024"},{"key":"2026090915261870000_qsag016-B18","author":"European Union Reference Laboratory for Single Residue Methods (EURL-SRM)","year":"2023"},{"key":"2026090915261870000_qsag016-B19","author":"European Commission","year":"2021"},{"key":"2026090915261870000_qsag016-B20","doi-asserted-by":"publisher","first-page":"462038","DOI":"10.1016\/j.chroma.2021.462038","volume":"1642","author":"Gormez","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"2026090915261870000_qsag016-B21","doi-asserted-by":"publisher","first-page":"101306","DOI":"10.1016\/j.mex.2021.101306","volume":"8","author":"Cutillas","year":"2021","journal-title":"MethodsX"},{"key":"2026090915261870000_qsag016-B22","doi-asserted-by":"publisher","first-page":"538","DOI":"10.38212\/2224-6614.3420","volume":"30","author":"Liao","year":"2022","journal-title":"J. Food Drug Anal"}],"container-title":["Journal of AOAC INTERNATIONAL"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jaoac\/advance-article-pdf\/doi\/10.1093\/jaoacint\/qsag016\/67408951\/qsag016.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/109\/5\/688\/67408951\/qsag016.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaoac\/article-pdf\/109\/5\/688\/67408951\/qsag016.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T19:26:24Z","timestamp":1788981984000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/109\/5\/688\/8528962"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,27]]},"references-count":22,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,2,27]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jaoacint\/qsag016","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"value":"1060-3271","type":"print"},{"value":"1944-7922","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,2,27]]}}}