{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T20:05:38Z","timestamp":1784145938814,"version":"3.55.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Scientific Research Project of Traditional Chinese Medicine Administration of Shanxi Provincial Health Commission","award":["2024ZYY2C119"],"award-info":[{"award-number":["2024ZYY2C119"]}]},{"name":"Scientific Research Project of Shanxi Health Vocational College","award":["2024XYZYLY006-00"],"award-info":[{"award-number":["2024XYZYLY006-00"]}]},{"name":"Scientific Research Project of Shanxi Health Vocational College","award":["2024XYZYLY003-00"],"award-info":[{"award-number":["2024XYZYLY003-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Speranskia tuberculata is a perennial herbaceous plant that is used in traditional Chinese medicine (TCM) to dispel wind and dampness, promote blood circulation, and relieve pain. However, QC markers, mechanisms of action, and systematic screening of quality indicators are still lacking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This work sought to establish the HPLC fingerprint of S. tuberculata and to predict its Q-Markers by applying network pharmacology combined with molecular docking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, HPLC was used to establish fingerprints and conduct similarity evaluation. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis were used to suggest the differences among these fingerprints. The \u201ccomponent\u2013target\u2013pathway\u201d network relationships of characteristic components of S. tuberculata were constructed by network pharmacology. Molecular docking technology was used to analyze the binding affinity between Q-Markers and core targets, and the potential Q-Markers were predicted.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>HPLC characteristic fingerprints identified 19 common peaks, among which four were tentatively identified as chlorogenic acid, kaempferol, diosmetin, and amentoflavone. Through network pharmacological and molecular docking analysis, it was predicted that kaempferol and diosmetin could serve as the Q-Markers of S. tuberculata.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The method established in this study is accurate, reliable, simple, and practical, and can be used as a reference method for S. tuberculata quality detection. Two Q-Markers selected by network pharmacology and molecular docking analysis can provide support and references for S. tuberculata QC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>This study selected S. tuberculata as the research object and, using the quality assessment of the authentic herbal source as the logical starting point, implemented the \u2018medicinal material characteristics\u2014chemical quality\u2014activity prediction' framework to fill the existing research gap in Q-Marker identification. The methodologies and strategies presented in this study can be extended to other traditional Chinese medicines, offering novel insights into the quantitative evaluation and identification of Q-Markers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsag043","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:40:51Z","timestamp":1779190851000},"source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive analysis of quality markers in\n                    <i>Speranskia tuberculata<\/i>\n                    based on HPLC fingerprinting, chemometrics, and network pharmacology"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9981-4313","authenticated-orcid":false,"given":"Huifang","family":"Li","sequence":"first","affiliation":[{"name":"Shanxi Health Vocational College, School of Pharmacy , Jinzhong, Shanxi Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2388-2300","authenticated-orcid":false,"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanxi Health Vocational College, School of Pharmacy , Jinzhong, Shanxi Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2557-2068","authenticated-orcid":false,"given":"Chun","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanxi Health Vocational College, School of Pharmacy , Jinzhong, Shanxi Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8934-3358","authenticated-orcid":false,"given":"Xinshu","family":"Wang","sequence":"additional","affiliation":[{"name":"Changzhi Medical College, The First Clinical Medical College , Changzhi, Shanxi Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7767-8049","authenticated-orcid":false,"given":"Naiyi","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanxi Health Vocational College, School of Pharmacy , Jinzhong, Shanxi Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"2026071515412675000_qsag043-B1","first-page":"1960","volume":"28","author":"Zhang","year":"2010","journal-title":"Chin. 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