{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:14:35Z","timestamp":1788380075291,"version":"build-2803163510"},"reference-count":24,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Department of Science and Technology"},{"name":"DST-INSPIRE","award":["IF210087"],"award-info":[{"award-number":["IF210087"]}]},{"DOI":"10.13039\/501100009975","name":"Charotar University of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009975","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Tofacitinib (TFC) is a Janus kinase inhibitor approved by the FDA, for the treatment of autoimmune disorders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The research aimed to develop, validate, and apply a simple, rugged, and environmentally sustainable reversed-phase (RP)-HPLC method for the quantitative estimation of TFC in in-house developed mesoporous silica nanoparticles (MCM-41 and MCM-48), intended for formulation-related analytical applications, while systematically evaluating environmental and analytical sustainability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>The chromatographic separation was achieved on an Inertsil\u00ae C18 column using an isocratic mobile phase of Milli-Q water\u2013acetonitrile (65\u2009+\u200935, v\/v) at a flow rate of 1\u2009mL\/min, with photodiode array (PDA) detection at 287\u2009nm and a total run time of 15\u2009min. The validated approach was applied to formulation-based analytical studies. Environmental and analytical sustainability was evaluated using the Analytical GREEnness (AGREE) and the Red-Green-Blue-12 (RGB-12) whiteness model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The method exhibited excellent specificity without interference from formulation excipients and showed linearity over 1\u201324\u2009\u00b5g\/mL (r\u00b2 = 0.9987). High accuracy (98\u2013102%), precision 0.06\u20131.31% RSD, and sensitivity ( 0.28\u2009\u00b5g\/mL LOD and 0.848\u2009\u00b5g\/mL LOQ) in compliance with International Conference on Harmonisation (ICH) guidelines, enabled reliable estimation of TFC. The method demonstrated good ruggedness (0.012\u20131.33% RSD), indicating reliability under small deliberate changes. High AGREE and RGB-12 whiteness (0.79 and 87.0) values confirmed the enhanced sustainability and practical feasibility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The developed RP-HPLC method integrates reliable analytical performance with green and white chemistry principles, rendering it suitable for routine QC and formulation-oriented research involving mesoporous silica-based drug delivery systems.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>A novel sustainable RP-HPLC method was developed and validated for the analysis of TFC-loaded MCM-41 and MCM-48 MSNs. The method demonstrated excellent performance in accordance with ICH Q2 (R2) guidelines. It was successfully applied for drug content determination and in vitro diffusion studies. Sustainability assessment using AGREE and RGB-12 models confirmed favorable greenness and whiteness, with reduced analysis time and resource consumption compared to reported methods.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsag073","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:47:12Z","timestamp":1785930432000},"source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of a sustainable RP-HPLC method for tofacitinib quantification in MCM-41 and MCM-48 silica nanoparticles and its in vitro application"],"prefix":"10.1093","author":[{"given":"Vidhi S","family":"Pandya","sequence":"first","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi R","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish N","family":"Nandpal","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6361-4865","authenticated-orcid":false,"given":"Meghana N","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9700-4942","authenticated-orcid":false,"given":"Bhupendra G","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Parul Institute of Pharmacy, Faculty of Pharmacy, Parul University, Department of Pharmaceutics, Waghodia-391760, Vadodara, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit A","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4481-8296","authenticated-orcid":false,"given":"Samir G","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8442-0349","authenticated-orcid":false,"given":"Ravish J","family":"Patel","sequence":"additional","affiliation":[{"name":"Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, CHARUSAT Campus, Changa-388421, Anand, Gujarat,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"2026090215214977500_qsag073-B1","doi-asserted-by":"publisher","first-page":"106480","DOI":"10.1016\/j.intimp.2020.106480","volume":"83","author":"Zhao","year":"2020","journal-title":"Int. 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