{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T23:29:43Z","timestamp":1794526183923,"version":"4.9.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Veterinary drug residues, pesticides, and chemical contaminants in animal-derived foods pose public health risks, including antimicrobial resistance development and consumer exposure to banned or withdrawn substances. Conventional targeted methods are inherently limited to predefined compounds and may overlook emerging or unexpected contaminants. Many developing countries still lack comprehensive multi-residue surveillance across diverse food matrices, highlighting the need for analytical frameworks adaptable to regulatory laboratories.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate an LC\u2013quadrupole-Orbitrap high-resolution mass spectrometry (LC\u2013Q-Orbitrap HRMS) framework combining reference-standard-assisted confirmation with non-targeted screening for the detection and identification of veterinary drug residues, pesticides, and chemical contaminants across multiple animal-derived food matrices.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A streamlined extraction protocol coupled with LC\u2013Q-Orbitrap HRMS was applied to 450 retail samples collected in Egypt, covering nine matrices (bovine liver, kidney, meat, heart, raw bovine milk, chicken breast, chicken eggs, honey, and Nile tilapia [Oreochromis niloticus]). Confirmation used 36 certified reference standards; non-targeted screening used Compound Discoverer\u2122 with spectral database matching and in silico fragmentation prediction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Nine compounds were confirmed at Level 1 (confirmed structure via reference standard match) according to the Schymanski identification confidence framework, accounting for 74 detection events, most frequently oxytetracycline (18 detections) and trimethoprim (17 detections). Non-targeted screening revealed 45 additional compounds identified at Level 2a (probable structure based on library spectrum match), including antimicrobials, \u03b2-agonists, pesticides, and pharmaceuticals. Phenacetin and tapentadol were each detected in 48 samples, and fenobucarb in 30% of milk samples. Liver and honey showed the greatest diversity of detected compounds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HRMS-based non-targeted screening extends analytical coverage beyond predefined reference-standard panels in multi-matrix food surveillance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>LC\u2013Q-Orbitrap HRMS detected 54 compounds across nine animal-derived food matrices\u2014nine Level 1 confirmations and 45 Level 2a probable structures\u2014extending chemical surveillance beyond reference-standard panels.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jaoacint\/qsag095","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:51:25Z","timestamp":1792065085000},"source":"Crossref","is-referenced-by-count":0,"title":["Non-targeted LC\u2013Q-Orbitrap HRMS screening of veterinary drugs, pesticides, and emerging contaminants in animal-derived foods and honey"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8928-5999","authenticated-orcid":false,"given":"Omar","family":"Khaled","sequence":"first","affiliation":[{"name":"Ministry of Agriculture, Agriculture Research Centre, Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Foods , Giza 12311,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3272-7688","authenticated-orcid":false,"given":"Lamia","family":"Ryad","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture, Agriculture Research Centre, Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Foods , Giza 12311,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3611-4854","authenticated-orcid":false,"given":"Fawzy","family":"Eissa","sequence":"additional","affiliation":[{"name":"Al-Azhar University, Environment and Bio-Agriculture Department, Faculty of Agriculture , Nasr City, Cairo 11884,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2112-2085","authenticated-orcid":false,"given":"Vinoth Kumar","family":"Ponnusamy","sequence":"additional","affiliation":[{"name":"Kaohsiung Medical University, Department of Medicinal and Applied Chemistry , Kaohsiung city 80708,","place":["Taiwan"]},{"name":"Kaohsiung Medical University, Research Center for Precision Environmental Medicine , Kaohsiung city 80708,","place":["Taiwan"]},{"name":"Kaohsiung Medical University Hospital, Kaohsiung Medical University, Department of Medical Research , Kaohsiung city 80708,","place":["Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-4666","authenticated-orcid":false,"given":"Mohamed","family":"Medhat","sequence":"additional","affiliation":[{"name":"MESLO Egypt and North Africa for Scientific Equipment, Chromatography and Mass Spectrometry Department , Heliopolis, Cairo 11341,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"2026111218124478200_qsag095-B1","doi-asserted-by":"publisher","first-page":"105851","DOI":"10.1016\/j.microc.2020.105851","volume":"162","author":"Castillo-Aguirre","year":"2021","journal-title":"Microchem. 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