{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T22:46:13Z","timestamp":1790549173495,"version":"4.1.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Minnesota Agricultural Experiment Station Hatch","award":["MIN-16-112"],"award-info":[{"award-number":["MIN-16-112"]}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["MIN-63-118"],"award-info":[{"award-number":["MIN-63-118"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The enteric pathogen Lawsonia intracellularis is one of the main causes of diarrhea and compromised weight gain in pigs worldwide. Traditional cell-line cultures have been used to study L. intracellularis pathogenesis. However, these systems fail to reproduce the epithelial changes observed in the intestines of L. intracellularis-infected pigs, specifically, the changes in intestinal cell constitution and gene expression. A more physiologically accurate and state-of-the-art model is provided by swine enteroids derived from stem cell-containing crypts from healthy pigs. The objective of this study was to verify the feasibility of two-dimensional swine enteroids as in vitro models for L. intracellularis infection. We established both three- and two-dimensional swine enteroid cultures derived from intestinal crypts. The two-dimensional swine enteroids were infected by L. intracellularis in four independent experiments. Enteroid-infected samples were collected 3 and 7 d postinfection for analysis using real-time quantitative PCR and L. intracellularis immunohistochemistry. In this study, we show that L. intracellularis is capable of infecting and replicating intracellularly in two-dimensional swine enteroids derived from ileum.<\/jats:p>","DOI":"10.1093\/jas\/skaa011","type":"journal-article","created":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T09:13:05Z","timestamp":1579165985000},"source":"Crossref","is-referenced-by-count":29,"title":["Evaluation of swine enteroids as in vitro models for\n                    <i>Lawsonia intracellularis<\/i>\n                    infection1,2"],"prefix":"10.1093","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7705-714X","authenticated-orcid":false,"given":"Talita Pilar","family":"Resende","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramya Lekha","family":"Medida","sequence":"additional","affiliation":[{"name":"Department of Animal Science, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio A","family":"Vannucci","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5848-0092","authenticated-orcid":false,"given":"Milena","family":"Saqui-Salces","sequence":"additional","affiliation":[{"name":"Department of Animal Science, College of Food, Agricultural and Natural Resource Sciences, University of Minnesota, St. Paul, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie","family":"Gebhart","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN"},{"name":"Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,16]]},"reference":[{"key":"2021022523380560700_CIT0001","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.vetimm.2015.08.005","article-title":"Lawsonia intracellularis infection of intestinal crypt cells is associated with specific depletion of secreted MUC2 in goblet cells","volume":"168","author":"Bengtsson","year":"2015","journal-title":"Vet. 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